Q9XTC6 (MOM4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase kinase kinase mom-4
EC=2.7.11.25

Gene names

Name:	mom-4
ORF Names:	F52F12.3

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the Wnt signaling pathway essential for the specification of the mesodermal cell fate in early embryos. Stimulates the wrm-1/lit-1-dependent phosphorylation of pop-1 and plays a role in the initial nuclear accumulation of wrm-1. Ref.1 Ref.2 Ref.4 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB O43318
Cofactor	Magnesium By similarity. UniProtKB O43318
Subunit structure	Interacts with, and is activated by, tap-1. Ref.2 Ref.5
Developmental stage	Expressed in the embryo. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. UniProtKB O43318 Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	MAPKKK cascade Inferred from direct assay Ref.2. Source: WormBase Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW asymmetric cell division Inferred from mutant phenotype Ref.2. Source: WormBase asymmetric protein localization involved in cell fate determination Inferred from mutant phenotype Ref.2. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase endodermal cell fate specification Inferred from mutant phenotype Ref.2. Source: WormBase muscle cell fate specification Inferred from mutant phenotype Ref.2. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from direct assay Ref.2. Source: WormBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.2. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Mitogen-activated protein kinase kinase kinase mom-4		PRO_0000351145
Regions
	Domain	51 – 305	255	Protein kinase
	Nucleotide binding	57 – 65	9	ATP By similarity UniProtKB O43318
	Compositional bias	10 – 19	10	Poly-Ser
	Compositional bias	424 – 427	4	Poly-Pro
Sites
	Active site	176	1	Proton acceptor By similarity UniProtKB O43318
	Binding site	84	1	ATP By similarity UniProtKB O43318

Sequences

	Sequence		Length	Mass (Da)
	Q9XTC6 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 686DD6C3A91F7EB9	FASTA	536	61,543
	10 20 30 40 50 60 MDNSSQSKPS SSSSSHSPSP AAITPTQRTT RDSGLCSTID IPEIQAQCID NLNSHYLGKG 70 80 90 100 110 120 TYGLVEKTRY RKTRQDDFRP AAIKYSSQLH MATLIREAKV MWDLRNHPNI IKIYGLYKSP 130 140 150 160 170 180 RNGQGVVMEY MDCGSMADLL YDRTHINYTI DHVASWMFQL SSAVDFFHSN SQVHRDLKLQ 190 200 210 220 230 240 NMLLSDRYRT MKLCDFGTFT SMHQSMTSNR GTPITMAPEV FRCEQYNMKS DIYSIGIIMW 250 260 270 280 290 300 QIIARNHPYR RDLSVPGLLY NVATANLRPQ ELECNPILSE FYKKCWNDNA DIRPTSSECV 310 320 330 340 350 360 EYFTLLKDEY PNGSVPLSDS STNGPAETPP PHAHRPTMLG TSSGSGIGSN NRTPTASKLL 370 380 390 400 410 420 NPQQPGQGHR RNRSETFVVQ PDLPYPTVPG EAGASRIPKS QSEAKNFRDR AKSEQRQPHR 430 440 450 460 470 480 DARPPPPFEH RRDSNDEEKH AVFMNICSNE ETRPIDPDTR DEKSLEIFHQ HCDNNKQYAD 490 500 510 520 530 AWVIKKEVMR AKHELIAQWP QHDHTVELLE RKYYLEQEIA RYRDIQDDNY FSTERM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MOM-4, a MAP kinase kinase kinase-related protein, activates WRM-1/LIT-1 kinase to transduce anterior/posterior polarity signals in C. elegans." Shin T.H., Yasuda J., Rocheleau C.E., Lin R., Soto M., Bei Y., Davis R.J., Mello C.C. Mol. Cell 4:275-280(1999) [PubMed: 10488343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE. Strain: Bristol N2.
[2]	"MAP kinase and Wnt pathways converge to downregulate an HMG-domain repressor in Caenorhabditis elegans." Meneghini M.D., Ishitani T., Carter J.C., Hisamoto N., Ninomiya-Tsuji J., Thorpe C.J., Hamill D.R., Matsumoto K., Bowerman B. Nature 399:793-797(1999) [PubMed: 10391246] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TAP-1. Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[4]	"Wnt signaling polarizes an early C. elegans blastomere to distinguish endoderm from mesoderm." Thorpe C.J., Schlesinger A., Carter J.C., Bowerman B. Cell 90:695-705(1997) [PubMed: 9288749] [Abstract] Cited for: FUNCTION.
[5]	"An evolutionarily conserved motif in the TAB1 C-terminal region is necessary for interaction with and activation of TAK1 MAPKKK." Ono K., Ohtomo T., Sato S., Sugamata Y., Suzuki M., Hisamoto N., Ninomiya-Tsuji J., Tsuchiya M., Matsumoto K. J. Biol. Chem. 276:24396-24400(2001) [PubMed: 11323434] [Abstract] Cited for: INTERACTION WITH TAP-1.
[6]	"Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos." Nakamura K., Kim S., Ishidate T., Bei Y., Pang K., Shirayama M., Trzepacz C., Brownell D.R., Mello C.C. Genes Dev. 19:1749-1754(2005) [PubMed: 16077004] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF143242 mRNA. Translation: AAD37359.1. AF145377 mRNA. Translation: AAD39816.1. Z83228 Genomic DNA. Translation: CAB60998.1.
RefSeq	NP_492620.1. NM_060219.6.
UniGene	Cel.18370.
3D structure databases
HSSP	HSSP built from PDB template 2EVA based on UniProtKB O43318.
ProteinModelPortal	Q9XTC6.
SMR	Q9XTC6. Positions 54-313.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26637N.
IntAct	Q9XTC6. 1 interaction.
MINT	MINT-1092643.
STRING	Q9XTC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F52F12.3.1; F52F12.3.1; F52F12.3. F52F12.3.2; F52F12.3.2; F52F12.3.
GeneID	172842.
KEGG	cel:F52F12.3.
NMPDR	fig|6239.3.peg.2653.
UCSC	F52F12.3. c. elegans.
Organism-specific databases
CTD	172842.
WormBase	F52F12.3; CE24994; WBGene00003396; mom-4.
Phylogenomic databases
eggNOG	meNOG06392.
GeneTree	EMGT00050000001111.
HOGENOM	HBG379074.
InParanoid	Q9XTC6.
OMA	IDHVASW.
PhylomeDB	Q9XTC6.
Gene expression databases
ArrayExpress	Q9XTC6.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR001245. Ser-Thr/Tyr_kinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	877235.

Entry information

Entry name

MOM4_CAEEL

Accession

Primary (citable) accession number: Q9XTC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	November 1, 1999
Last modified:	October 19, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project