Reviewed, UniProtKB/Swiss-Prot Q9XTC6 (MOM4_CAEEL)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase mom-4
    EC=2.7.11.25

Gene names

Name:	mom-4
ORF Names:	F52F12.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the Wnt signaling pathway essential for the specification of the mesodermal cell fate in early embryos. Stimulates the wrm-1/lit-1-dependent phosphorylation of pop-1 and plays a role in the initial nuclear accumulation of wrm-1. Ref.1 Ref.2 Ref.4 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB O43318
Cofactor	Magnesium By similarity. UniProtKB O43318
Subunit structure	Interacts with, and is activated by, tap-1. Ref.2 Ref.5
Developmental stage	Expressed in the embryo. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nhr-6	P41829	1	EBI-322902,EBI-314469

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Mitogen-activated protein kinase kinase kinase mom-4		PRO_0000351145
Regions
	Domain	51 – 305	255	Protein kinase
	Nucleotide binding	57 – 65	9	ATP By similarity UniProtKB O43318
	Compositional bias	10 – 19	10	Poly-Ser
	Compositional bias	424 – 427	4	Poly-Pro
Sites
	Active site	176	1	Proton acceptor By similarity UniProtKB O43318
	Binding site	84	1	ATP By similarity UniProtKB O43318

Sequences

	Sequence		Length	Mass (Da)
	Q9XTC6-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 686DD6C3A91F7EB9	FASTA	536	61,543
	10 20 30 40 50 60 MDNSSQSKPS SSSSSHSPSP AAITPTQRTT RDSGLCSTID IPEIQAQCID NLNSHYLGKG 70 80 90 100 110 120 TYGLVEKTRY RKTRQDDFRP AAIKYSSQLH MATLIREAKV MWDLRNHPNI IKIYGLYKSP 130 140 150 160 170 180 RNGQGVVMEY MDCGSMADLL YDRTHINYTI DHVASWMFQL SSAVDFFHSN SQVHRDLKLQ 190 200 210 220 230 240 NMLLSDRYRT MKLCDFGTFT SMHQSMTSNR GTPITMAPEV FRCEQYNMKS DIYSIGIIMW 250 260 270 280 290 300 QIIARNHPYR RDLSVPGLLY NVATANLRPQ ELECNPILSE FYKKCWNDNA DIRPTSSECV 310 320 330 340 350 360 EYFTLLKDEY PNGSVPLSDS STNGPAETPP PHAHRPTMLG TSSGSGIGSN NRTPTASKLL 370 380 390 400 410 420 NPQQPGQGHR RNRSETFVVQ PDLPYPTVPG EAGASRIPKS QSEAKNFRDR AKSEQRQPHR 430 440 450 460 470 480 DARPPPPFEH RRDSNDEEKH AVFMNICSNE ETRPIDPDTR DEKSLEIFHQ HCDNNKQYAD 490 500 510 520 530 AWVIKKEVMR AKHELIAQWP QHDHTVELLE RKYYLEQEIA RYRDIQDDNY FSTERM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MOM-4, a MAP kinase kinase kinase-related protein, activates WRM-1/LIT-1 kinase to transduce anterior/posterior polarity signals in C. elegans." Shin T.H., Yasuda J., Rocheleau C.E., Lin R., Soto M., Bei Y., Davis R.J., Mello C.C. Mol. Cell 4:275-280(1999) [PubMed: 10488343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE. Strain: Bristol N2.
[2]	"MAP kinase and Wnt pathways converge to downregulate an HMG-domain repressor in Caenorhabditis elegans." Meneghini M.D., Ishitani T., Carter J.C., Hisamoto N., Ninomiya-Tsuji J., Thorpe C.J., Hamill D.R., Matsumoto K., Bowerman B. Nature 399:793-797(1999) [PubMed: 10391246] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TAP-1. Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[4]	"Wnt signaling polarizes an early C. elegans blastomere to distinguish endoderm from mesoderm." Thorpe C.J., Schlesinger A., Carter J.C., Bowerman B. Cell 90:695-705(1997) [PubMed: 9288749] [Abstract] Cited for: FUNCTION.
[5]	"An evolutionarily conserved motif in the TAB1 C-terminal region is necessary for interaction with and activation of TAK1 MAPKKK." Ono K., Ohtomo T., Sato S., Sugamata Y., Suzuki M., Hisamoto N., Ninomiya-Tsuji J., Tsuchiya M., Matsumoto K. J. Biol. Chem. 276:24396-24400(2001) [PubMed: 11323434] [Abstract] Cited for: INTERACTION WITH TAP-1.
[6]	"Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos." Nakamura K., Kim S., Ishidate T., Bei Y., Pang K., Shirayama M., Trzepacz C., Brownell D.R., Mello C.C. Genes Dev. 19:1749-1754(2005) [PubMed: 16077004] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF143242 mRNA. Translation: AAD37359.1. AF145377 mRNA. Translation: AAD39816.1. Z83228 Genomic DNA. Translation: CAB60998.1.
RefSeq	NP_492620.1.
UniGene	Cel.18370
3D structure databases
HSSP	HSSP built from PDB template 2EVA based on UniProtKB O43318.
SMR	Q9XTC6. Positions 24-298, 55-303.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26637N.
IntAct	Q9XTC6. 5 interactions.
STRING	Q9XTC6.
Genome annotation databases
Ensembl	F52F12.3; F52F12.3; F52F12.3; Caenorhabditis elegans. [Genome view]
GeneID	172842.
KEGG	cel:F52F12.3.
NMPDR	fig|6239.3.peg.2653.
UCSC	F52F12.3. c. elegans.
Organism-specific databases
CTD	172842.
WormBase	WBGene00003396. mom-4.
WormPep	F52F12.3. CE24994. [WorfDB]
Phylogenomic databases
eggNOG	meNOG06392.
HOGENOM	HBG379074.
InParanoid	Q9XTC6.
OMA	IREAKVM.
PhylomeDB	Q9XTC6.
Gene expression databases
ArrayExpress	Q9XTC6.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	877235.

Entry information

Entry name

MOM4_CAEEL

Accession

Primary (citable) accession number: Q9XTC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project