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Protein

Prolyl endopeptidase

Gene

PREP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei554 – 5541Charge relay systemPROSITE-ProRule annotation
Active sitei641 – 6411Charge relay systemPROSITE-ProRule annotation
Active sitei680 – 6801Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type exopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS09.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl endopeptidase (EC:3.4.21.26)
Short name:
PE
Alternative name(s):
Post-proline cleaving enzyme
Gene namesi
Name:PREP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 710710Prolyl endopeptidasePRO_0000122400Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei157 – 1571N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ9XTA2.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ9XTA2.
SMRiQ9XTA2. Positions 1-709.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.Curated

Phylogenomic databases

eggNOGiCOG1505.
HOGENOMiHOG000238967.
HOVERGENiHBG007251.
InParanoidiQ9XTA2.
KOiK01322.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XTA2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSFQYPDVY RDETAVQDYH GHKICDPYAW LEDPDSEQTK AFVEAQNKIT
60 70 80 90 100
VPFLEQCPIR GLYKERMTEL YDYPKYSCNF KKGKRYFYFY NTGLQNQRVL
110 120 130 140 150
YVQDSLEGEA RVCLDPNTLS DDGTVALRGY AFSEDGEYVA YGLSASGSDW
160 170 180 190 200
VTIKFMKVDG AKELADVLER VKFSCMAWTH DGKGMFYNAY PQQDGKSDGT
210 220 230 240 250
ETSTNLHQKL CYHVLGTDQS EDILCAEFPD EPKWMGGAEL SDDGRYVLLS
260 270 280 290 300
IREGCDPVNR LWYCDLHQEP NGITGILKWV KLIDNFEGEY DYVTNEGTVF
310 320 330 340 350
TFKTNRHSPN YRLINIDFTD PEESRWKVLV PEHEKDVLEW VACVRSNFLV
360 370 380 390 400
LCYLHDVKNT LQLHDMATGA LLKTFPLEVG SVVGYSGQKK DTEIFYQFTS
410 420 430 440 450
FLSPGIIYHC DLTKEELEPR VFREVTVKGI DASDYQTVQI FYPSKDGTKI
460 470 480 490 500
PMFIVHKKGI KLDGSHPAFL YGYGGFNISI TPNYSVCRLI FVRHMGGVLA
510 520 530 540 550
VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE GYTSPKRLTI
560 570 580 590 600
NGGSNGGLLV ATCANQRPDL FGCVIAQVGV MDMLKFHKYT IGHAWTTDYG
610 620 630 640 650
CSDNKQHFEW LIKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSP
660 670 680 690 700
KFIATLQHLV GRSRKQNNPL LIHVDTKAGH GAGKPTAKVI EEVSDMFAFI
710
ARCLNIDWIQ
Length:710
Mass (Da):80,641
Last modified:November 1, 1999 - v1
Checksum:iCB2E7F0DAE2CFE9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028866 mRNA. Translation: BAA78907.1.
PIRiJC5877.
RefSeqiNP_777197.1. NM_174772.1.
UniGeneiBt.4796.

Genome annotation databases

GeneIDi286818.
KEGGibta:286818.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028866 mRNA. Translation: BAA78907.1.
PIRiJC5877.
RefSeqiNP_777197.1. NM_174772.1.
UniGeneiBt.4796.

3D structure databases

ProteinModelPortaliQ9XTA2.
SMRiQ9XTA2. Positions 1-709.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ9XTA2.
ChEMBLiCHEMBL2460.

Protein family/group databases

MEROPSiS09.001.

Proteomic databases

PRIDEiQ9XTA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi286818.
KEGGibta:286818.

Organism-specific databases

CTDi5550.

Phylogenomic databases

eggNOGiCOG1505.
HOGENOMiHOG000238967.
HOVERGENiHBG007251.
InParanoidiQ9XTA2.
KOiK01322.

Miscellaneous databases

NextBioi20806472.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the cDNA encoding prolyl oligopeptidase (prolyl endopeptidase) from bovine brain."
    Yoshimoto T., Miyazaki K., Haraguchi N., Kitazono A., Kabashima T., Ito K.
    Biol. Pharm. Bull. 20:1047-1050(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiPPCE_BOVIN
AccessioniPrimary (citable) accession number: Q9XTA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.