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Reviewed, UniProtKB/Swiss-Prot Q9XTA0 (DOPO_HORSE)

Last modified June 16, 2009. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Dopamine beta-hydroxylase
    EC=1.14.17.1
Cleaved into the following chain:
    1- Recommended name:
            Soluble dopamine beta-hydroxylase
Gene names
Name: DBH
OrganismEquus caballus (Horse)
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

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Protein attributes

Sequence length610 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Conversion of dopamine to noradrenaline By similarity.

Catalytic activity

3,4-dihydroxyphenethylamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O.

Cofactor

Binds 1 PQQ per subunit By similarity.

Binds 2 copper ions per subunit By similarity.

Pathway

Catecholamine biosynthesis; norepinephrine biosynthesis; norepinephrine from dopamine: step 1/1.

Subunit structure

Homotetramer composed of two non-covalently bound disulfide-linked dimers By similarity.

Subcellular location

Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle › secretory vesicle lumen By similarity. Cytoplasmic vesicle › secretory vesicle › chromaffin granule lumen By similarity.

Cytoplasmic vesicle › secretory vesicle membrane; Single-pass type II membrane protein By similarity. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the copper type II ascorbate-dependent monooxygenase family.

Contains 1 DOMON domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 610610Dopamine beta-hydroxylase
PRO_0000305213
Chain33 – 610578Soluble dopamine beta-hydroxylase Potential
PRO_0000308208

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 3021Signal-anchor for type II membrane Potential
Topological domain31 – 610580Intragranular Potential
Domain50 – 166117DOMON

Sites

Active site2231 Potential
Active site4051 Potential
Metal binding2551Copper A By similarity
Metal binding2561Copper A By similarity
Metal binding3261Copper A By similarity
Metal binding4051Copper B By similarity
Metal binding4071Copper B By similarity
Metal binding4801Copper B By similarity

Amino acid modifications

Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation1941N-linked (GlcNAc...) Potential
Disulfide bond147 ↔ 589 By similarity
Disulfide bond225 ↔ 276 By similarity
Disulfide bond262 ↔ 288 By similarity
Disulfide bond383 ↔ 496 By similarity
Disulfide bond387 ↔ 558 By similarity
Disulfide bond459 ↔ 481 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9XTA0-1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 09B6095DD0551D8D

FASTA61068,229
        10         20         30         40         50         60 
MKVPSPSVRE AASMYGTAVA IFLVILVAAL QGSEPPESPF PYRIPLDPEG TLELSWNVSY 

        70         80         90        100        110        120 
VQETIHFQLL VRELKAGVLF GMSDRGELEN ADLVVLWTDG DSAYFGDAWS DQKGWIHLDA 

       130        140        150        160        170        180 
QQDYQLLRAQ RTPEGLSLLF KRPFGTCDPK DYLIEDGTVH LVYGILEEPF WSLEAINTSA 

       190        200        210        220        230        240 
LHTGLQRVQL LKPNVSVPAL PADMRTMEVR APDVLVPGQE TTYWCYITEL PDGFPRHHIV 

       250        260        270        280        290        300 
MYEPIVTEGN EALVHHMEVF QCAAEFESFP QFNGPCDSKM KPSRLNYCRN VLAAWALGAK 

       310        320        330        340        350        360 
AFYYPEEAGL AFGGAGSSRF LRLEVHYHNP LKIEGRRDSS GIRLYYTATL RRFDAGIMEL 

       370        380        390        400        410        420 
GLVYTPVMAI PPQETAFVLT GYCTDKCTQL ALPPSGIHIF ASQLHTHLTG RKVVTVLARD 

       430        440        450        460        470        480 
GREREVVNRD DHYSPHFQEI RMLKKVVSVH PGDVLITSCT YNTEDRKLAT VGGFGILEEM 

       490        500        510        520        530        540 
CVNYVHYYPQ TQLELCKSAV DPGFLQKYFH FVNRFNGEEV CTCPQASVPE QFATVPWNSF 

       550        560        570        580        590        600 
NRQVLSALYG FAPISMHCNR SSAVRFQGDW NLQPLPEIIS KLEEPTPRCP ASRGRSPAGP 

       610 
TVVDIGGGKG

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References

[1]"Molecular cloning, nucleotide sequence and presence of multiple functional polyadenylation signals in the 3'-untranslated region of equine dopamine beta-hydroxylase cDNA."
Sato F., Hasegawa T., Katayama Y., Ishida N.
DNA Seq. 13:257-262(2002) [PubMed: 12592705] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

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Cross-references

Sequence databases

AB029430 mRNA. Translation: BAA82274.1.
RefSeqNP_001075239.1.
UniGeneEca.13026

3D structure databases

HSSPHSSP built from PDB template 1PHM based on UniProtKB P14925.
ModBaseSearch...

Genome annotation databases

EnsemblENSECAG00000019582. Equus caballus. [Contig view]
GeneID791247.
KEGGecb:791247.

Phylogenomic databases

HOVERGENQ9XTA0.

Enzyme and pathway databases

BRENDA1.14.17.1. 1464.

Family and domain databases

InterProIPR014784. Cu2_ascorb_mOase-like_C.
IPR014783. Cu2_ascorb_mOase_C.
IPR000323. Cu2_ascorb_mOase_N.
IPR013050. DOMON.
IPR005018. DOMON_rel.
IPR000945. Dopamine_b_mOase.
[Graphical view]
Gene3DG3DSA:2.60.120.230. Cu2_ascorb_mOase_core. 1 hit.
G3DSA:2.60.120.310. Cu2_ascorb_mOase_core. 1 hit.
PANTHERPTHR10157. Dopamine_b_mOase. 1 hit.
PfamPF03712. Cu2_monoox_C. 1 hit.
PF01082. Cu2_monooxygen. 1 hit.
PF03351. DOMON. 1 hit.
[Graphical view]
PRINTSPR00767. DBMONOXGNASE.
SMARTSM00664. DoH. 1 hit.
[Graphical view]
PROSITEPS00084. CU2_MONOOXYGENASE_1. 1 hit.
PS00085. CU2_MONOOXYGENASE_2. 1 hit.
PS50836. DOMON. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDOPO_HORSE
AccessionPrimary (citable) accession number: Q9XTA0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: November 1, 1999
Last modified: June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents