Reviewed,
UniProtKB/Swiss-Prot Q9XTA0 (DOPO_HORSE)
Last modified
June 16, 2009.
Version 38.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dopamine beta-hydroxylase EC=1.14.17.1 Cleaved into the following chain: 1- Recommended name: Soluble dopamine beta-hydroxylase | ||
| Gene names |
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| Organism | Equus caballus (Horse) | ||
| Taxonomic identifier | 9796 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus |
Protein attributes
| Sequence length | 610 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Conversion of dopamine to noradrenaline By similarity. |
| Catalytic activity | 3,4-dihydroxyphenethylamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O. |
| Cofactor | Binds 1 PQQ per subunit By similarity. Binds 2 copper ions per subunit By similarity. |
| Pathway | Catecholamine biosynthesis; norepinephrine biosynthesis; norepinephrine from dopamine: step 1/1. |
| Subunit structure | Homotetramer composed of two non-covalently bound disulfide-linked dimers By similarity. |
| Subcellular location | Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle › secretory vesicle lumen By similarity. Cytoplasmic vesicle › secretory vesicle › chromaffin granule lumen By similarity. Cytoplasmic vesicle › secretory vesicle membrane; Single-pass type II membrane protein By similarity. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass type II membrane protein By similarity. |
| Sequence similarities | Belongs to the copper type II ascorbate-dependent monooxygenase family. Contains 1 DOMON domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 610 | 610 | Dopamine beta-hydroxylase | PRO_0000305213 | |||||||
| Chain | 33 – 610 | 578 | Soluble dopamine beta-hydroxylase Potential | PRO_0000308208 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 9 | 9 | Cytoplasmic Potential | ||||||||
| Transmembrane | 10 – 30 | 21 | Signal-anchor for type II membrane Potential | ||||||||
| Topological domain | 31 – 610 | 580 | Intragranular Potential | ||||||||
| Domain | 50 – 166 | 117 | DOMON | ||||||||
Sites | |||||||||||
| Active site | 223 | 1 | Potential | ||||||||
| Active site | 405 | 1 | Potential | ||||||||
| Metal binding | 255 | 1 | Copper A By similarity | ||||||||
| Metal binding | 256 | 1 | Copper A By similarity | ||||||||
| Metal binding | 326 | 1 | Copper A By similarity | ||||||||
| Metal binding | 405 | 1 | Copper B By similarity | ||||||||
| Metal binding | 407 | 1 | Copper B By similarity | ||||||||
| Metal binding | 480 | 1 | Copper B By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 57 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 177 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 194 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 147 ↔ 589 | By similarity | |||||||||
| Disulfide bond | 225 ↔ 276 | By similarity | |||||||||
| Disulfide bond | 262 ↔ 288 | By similarity | |||||||||
| Disulfide bond | 383 ↔ 496 | By similarity | |||||||||
| Disulfide bond | 387 ↔ 558 | By similarity | |||||||||
| Disulfide bond | 459 ↔ 481 | By similarity | |||||||||
Sequences
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References
| [1] | "Molecular cloning, nucleotide sequence and presence of multiple functional polyadenylation signals in the 3'-untranslated region of equine dopamine beta-hydroxylase cDNA." Sato F., Hasegawa T., Katayama Y., Ishida N. DNA Seq. 13:257-262(2002) [PubMed: 12592705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| AB029430 mRNA. Translation: BAA82274.1. | |
| RefSeq | NP_001075239.1. |
| UniGene | Eca.13026 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1PHM based on UniProtKB P14925. |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | ENSECAG00000019582. Equus caballus. [Contig view] |
| GeneID | 791247. |
| KEGG | ecb:791247. |
Phylogenomic databases | |
| HOVERGEN | Q9XTA0. |
Enzyme and pathway databases | |
| BRENDA | 1.14.17.1. 1464. |
Family and domain databases | |
| InterPro | IPR014784. Cu2_ascorb_mOase-like_C. IPR014783. Cu2_ascorb_mOase_C. IPR000323. Cu2_ascorb_mOase_N. IPR013050. DOMON. IPR005018. DOMON_rel. IPR000945. Dopamine_b_mOase. [Graphical view] |
| Gene3D | G3DSA:2.60.120.230. Cu2_ascorb_mOase_core. 1 hit. G3DSA:2.60.120.310. Cu2_ascorb_mOase_core. 1 hit. |
| PANTHER | PTHR10157. Dopamine_b_mOase. 1 hit. |
| Pfam | PF03712. Cu2_monoox_C. 1 hit. PF01082. Cu2_monooxygen. 1 hit. PF03351. DOMON. 1 hit. [Graphical view] |
| PRINTS | PR00767. DBMONOXGNASE. |
| SMART | SM00664. DoH. 1 hit. [Graphical view] |
| PROSITE | PS00084. CU2_MONOOXYGENASE_1. 1 hit. PS00085. CU2_MONOOXYGENASE_2. 1 hit. PS50836. DOMON. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DOPO_HORSE | ||||||||
| Accession | Primary (citable) accession number: Q9XTA0 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


