Q9XT87 (LDHA_MONDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase A chain
Short name=LDH-A
EC=1.1.1.27

Alternative name(s):
LDH muscle subunit
Short name=LDH-M

Gene names

Name:

LDHA

Organism

Monodelphis domestica (Gray short-tailed opossum)

Taxonomic identifier

13616 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Metatheria › Didelphimorphia › Didelphidae › Monodelphis

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	ISGylated By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 332	331	L-lactate dehydrogenase A chain		PRO_0000168413
Regions
	Nucleotide binding	29 – 57	29	NAD By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	99	1	NAD By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	138	1	NAD or substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
Amino acid modifications
	Modified residue	5	1	N6-acetyllysine By similarity
	Modified residue	14	1	N6-acetyllysine By similarity
	Modified residue	57	1	N6-acetyllysine By similarity
	Modified residue	81	1	N6-acetyllysine By similarity
	Modified residue	118	1	N6-acetyllysine By similarity
	Modified residue	126	1	N6-acetyllysine By similarity
	Modified residue	239	1	Phosphotyrosine By similarity
	Modified residue	278	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XT87 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C9D11DA82504AA9D	FASTA	332	36,358
	10 20 30 40 50 60 MGTVKDQLIL NVLKEEQTPH NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG 70 80 90 100 110 120 EMMDLQHGSL FLKTPKIVSS KDYAVTANSK LVVITAGARQ QEGESRLNLV QRNVNIFKFI 130 140 150 160 170 180 IPNIVKYSPN CKLLVVSNPV DILTYVAWKL SGFPKNRVIG SGCNLDSARF RYLMGEKLGI 190 200 210 220 230 240 HSSSCHGWIL GEHGDSSVPV WSGVNVAGVS LKSLHPALGT DSDSEQWKDV HKQVVESAYE 250 260 270 280 290 300 VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI 310 320 330 SDVVKVTLTT EEESRLKQSA DTLWGIQKEL QF

References

[1]	"Molecular evolution of vertebrate lactate dehydrogenase isozymes by gene duplication." Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF070996 mRNA. Translation: AAD40733.1.
RefSeq	NP_001028147.1. NM_001032975.1. XP_003339625.1. XM_003339577.1.
UniGene	Mdm.239.
3D structure databases
ProteinModelPortal	Q9XT87.
SMR	Q9XT87. Positions 2-332.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9XT87.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMODT00000008256; ENSMODP00000008092; ENSMODG00000006522. ENSMODT00000024886; ENSMODP00000024452; ENSMODG00000019596.
GeneID	100012604. 503513.
KEGG	mdo:100012604. mdo:503513.
Organism-specific databases
CTD	3939.
Phylogenomic databases
eggNOG	maNOG08436.
GeneTree	ENSGT00550000074541.
HOVERGEN	HBG000462.
InParanoid	Q9XT87.
OMA	IHPLSCH.
OrthoDB	EOG4RR6HR.
PhylomeDB	Q9XT87.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00016.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHA_MONDO

Accession

Primary (citable) accession number: Q9XT87

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program