Reviewed, UniProtKB/Swiss-Prot Q9XT87 (LDHA_MONDO)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27
Alternative name(s):
    LDH muscle subunit
      Short name=LDH-M

Gene names

Name:

LDHA

Organism

Monodelphis domestica (Short-tailed gray opossum)

Taxonomic identifier

13616 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Metatheria › Didelphimorphia › Didelphidae › Monodelphis

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 332	331	L-lactate dehydrogenase A chain		PRO_0000168413
Regions
	Nucleotide binding	29 – 57	29	NAD By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	99	1	NAD By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	138	1	NAD or substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
Amino acid modifications
	Modified residue	239	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XT87-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C9D11DA82504AA9D	FASTA	332	36,358
	10 20 30 40 50 60 MGTVKDQLIL NVLKEEQTPH NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG 70 80 90 100 110 120 EMMDLQHGSL FLKTPKIVSS KDYAVTANSK LVVITAGARQ QEGESRLNLV QRNVNIFKFI 130 140 150 160 170 180 IPNIVKYSPN CKLLVVSNPV DILTYVAWKL SGFPKNRVIG SGCNLDSARF RYLMGEKLGI 190 200 210 220 230 240 HSSSCHGWIL GEHGDSSVPV WSGVNVAGVS LKSLHPALGT DSDSEQWKDV HKQVVESAYE 250 260 270 280 290 300 VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI 310 320 330 SDVVKVTLTT EEESRLKQSA DTLWGIQKEL QF

References

[1]	"Molecular evolution of vertebrate lactate dehydrogenase isozymes by gene duplication." Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF070996 mRNA. Translation: AAD40733.1.
RefSeq	NP_001028147.1. XP_001364556.1.
UniGene	Mdm.239
3D structure databases
HSSP	HSSP built from PDB template 1I10 based on UniProtKB P00338.
SMR	Q9XT87. Positions 2-332.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMODG00000006522. Monodelphis domestica. [Contig view] ENSMODG00000019596. Monodelphis domestica. [Contig view]
GeneID	100012604. 503513.
KEGG	mdo:100012604. mdo:503513.
Phylogenomic databases
HOVERGEN	Q9XT87.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

LDHA_MONDO

Accession

Primary (citable) accession number: Q9XT87

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 54 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)