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Protein

Voltage-gated potassium channel subunit beta-1

Gene

KCNAB1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (By similarity). Modulates action potentials via its effect on the pore-forming alpha subunits (By similarity). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Mediates closure of delayed rectifier potassium channels by physically obstructing the pore via its N-terminal domain and increases the speed of channel closure for other family members (By similarity). Promotes the closure of KCNA1, KCNA2 and KCNA5 channels (By similarity). Accelerates KCNA4 channel closure (By similarity). Accelerates the closure of heteromeric channels formed by KCNA1 and KCNA4 (By similarity). Accelerates the closure of heteromeric channels formed by KCNA2, KCNA5 and KCNA6 (By similarity). Enhances KCNB1 and KCNB2 channel activity (By similarity). Binds NADPH; this is required for efficient down-regulation of potassium channel activity (By similarity). Has NADPH-dependent aldoketoreductase activity (By similarity). Oxidation of the bound NADPH strongly decreases N-type inactivation of potassium channel activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115NADPBy similarity1
Binding sitei137NADPBy similarity1
Active sitei142Proton donor/acceptorBy similarity1
Binding sitei142NADPBy similarity1
Binding sitei266NADPBy similarity1
Binding sitei306NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi108 – 109NADPBy similarity2
Nucleotide bindingi240 – 241NADPBy similarity2
Nucleotide bindingi295 – 300NADPBy similarity6
Nucleotide bindingi375 – 381NADPBy similarity7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Oxidoreductase, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

NADP, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-gated potassium channel subunit beta-1 (EC:1.1.1.-By similarity)
Alternative name(s):
K(+) channel subunit beta-1
Kv-beta-1
Gene namesi
Name:KCNAB1
Synonyms:KVBETA1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Recruited to the cytoplasmic side of the cell membrane via its interaction with pore-forming potassium channel alpha subunits.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001487421 – 419Voltage-gated potassium channel subunit beta-1Add BLAST419

Expressioni

Tissue specificityi

Detected in portal vein myocytes (at protein level) (PubMed:11717160).

Interactioni

Subunit structurei

Homotetramer (By similarity). Interaction with tetrameric potassium channel alpha subunits gives rise to a heterooctamer (Probable). Identified in potassium channel complexes containing KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNAB1 and KCNAB2 (By similarity). Interacts with KCNA1 (PubMed:11717160). Interacts with the dimer formed by GNB1 and GNG2; this enhances KCNA1 binding (By similarity). Interacts with KCNA4 (By similarity). Interacts with KCNA5 (PubMed:11717160). Interacts with KCNB2 (By similarity). Interacts with SQSTM1 (By similarity). Part of a complex containing KCNA1, KCNA4 and LGI1; interaction with LGI1 inhibits down-regulation of KCNA1 channel activity (By similarity).By similarityCurated1 Publication

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006814.

Structurei

3D structure databases

ProteinModelPortaliQ9XT31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal domain of the beta subunit mediates closure of delayed rectifier potassium channels by physically obstructing the pore.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1575. Eukaryota.
COG0667. LUCA.
HOGENOMiHOG000250283.
HOVERGENiHBG052216.
InParanoidiQ9XT31.
KOiK04882.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR005983. K_chnl_volt-dep_bsu_KCNAB.
IPR005399. K_chnl_volt-dep_bsu_KCNAB-rel.
IPR005400. K_chnl_volt-dep_bsu_KCNAB1.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSiPR01578. KCNAB1CHANEL.
PR01577. KCNABCHANNEL.
SUPFAMiSSF51430. SSF51430. 1 hit.
TIGRFAMsiTIGR01293. Kv_beta. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform KvB1.3 (identifier: Q9XT31-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLAARTGAAG SQIAEESSKL RKQAAFSGGS KDRSPKKASE NVKDSSLSPS
60 70 80 90 100
GQSQVRARQL ALLREVEMNW YLKLCELSSE HTTAYTTGMP HRNLGKSGLR
110 120 130 140 150
VSCLGLGTWV TFGGQISDEV AERLMTIAYE SGVNLFDTAE VYAAGKAEVI
160 170 180 190 200
LGSIIKKKGW RRSSLVITTK LYWGGKAETE RGLSRKHIIE GLKGSLQRLQ
210 220 230 240 250
LEYVDVVFAN RPDSNTPMEE IVRAMTHVIN QGMAMYWGTS RWSAMEIMEA
260 270 280 290 300
YSVARQFNMI PPVCEQAEYH LFQREKVEVQ LPELYHKIGV GAMTWSPLAC
310 320 330 340 350
GIISGKYGNG VPESSRASLK CYQWLKERIV SEEGRKQQNK LKDLSPIAER
360 370 380 390 400
LGCTLPQLAV AWCLRNEGVS SVLLGSSTPE QLIENLGAIQ VLPKMTSHVV
410
NEIDNILRNK PYSKKDYRS
Length:419
Mass (Da):46,537
Last modified:November 1, 1999 - v1
Checksum:i721A8DA5087EB93C
GO
Isoform KvB1.1 (identifier: Q9XT31-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: MLAARTGAAG...TTAYTTGMPH → MQVSIACTEH...STAKQTGMKY

Show »
Length:401
Mass (Da):44,723
Checksum:i09F7860DCD3728B6
GO
Isoform KvB1.2 (identifier: Q9XT31-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: MLAARTGAAG...TTAYTTGMPH → MHLYKPACAD...TTKAETGMAY

Show »
Length:408
Mass (Da):45,585
Checksum:i0E4E9651D2E23000
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0010531 – 91MLAAR…TGMPH → MQVSIACTEHNLKSRNGEDR LLSKQSSNAPNVVNAARAKF RTVAIIARSLGTFTPQHHIS LKESTAKQTGMKY in isoform KvB1.1. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_0010521 – 91MLAAR…TGMPH → MHLYKPACADIPSPKLGLPK SSESALKCRRHLMVTKTQPQ AACWPVRPSGPTERKHLERF LCVHGVSLQETTKAETGMAY in isoform KvB1.2. 1 PublicationAdd BLAST91

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131934 mRNA. Translation: AAD37853.1.
AF131935 mRNA. Translation: AAD37854.1.
AF131936 mRNA. Translation: AAD37855.1.
RefSeqiNP_001164427.1. NM_001170956.1. [Q9XT31-1]
XP_008264420.1. XM_008266198.2. [Q9XT31-3]
UniGeneiOcu.2430.

Genome annotation databases

GeneIDi100328569.
KEGGiocu:100328569.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131934 mRNA. Translation: AAD37853.1.
AF131935 mRNA. Translation: AAD37854.1.
AF131936 mRNA. Translation: AAD37855.1.
RefSeqiNP_001164427.1. NM_001170956.1. [Q9XT31-1]
XP_008264420.1. XM_008266198.2. [Q9XT31-3]
UniGeneiOcu.2430.

3D structure databases

ProteinModelPortaliQ9XT31.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100328569.
KEGGiocu:100328569.

Organism-specific databases

CTDi7881.

Phylogenomic databases

eggNOGiKOG1575. Eukaryota.
COG0667. LUCA.
HOGENOMiHOG000250283.
HOVERGENiHBG052216.
InParanoidiQ9XT31.
KOiK04882.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR005983. K_chnl_volt-dep_bsu_KCNAB.
IPR005399. K_chnl_volt-dep_bsu_KCNAB-rel.
IPR005400. K_chnl_volt-dep_bsu_KCNAB1.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSiPR01578. KCNAB1CHANEL.
PR01577. KCNABCHANNEL.
SUPFAMiSSF51430. SSF51430. 1 hit.
TIGRFAMsiTIGR01293. Kv_beta. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCAB1_RABIT
AccessioniPrimary (citable) accession number: Q9XT31
Secondary accession number(s): Q9XT32, Q9XT33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1999
Last modified: October 5, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.