Reviewed, UniProtKB/Swiss-Prot Q9XT09 (MP2K1_PANTR)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 1
      Short name=MAP kinase kinase 1
      Short name=MAPKK 1
    EC=2.7.12.2
Alternative name(s):
    ERK activator kinase 1
    MAPK/ERK kinase 1
      Short name=MEK1

Gene names

Name:	MAP2K1
Synonyms:	MEK1, PRKMK1

Organism

Pan troglodytes (Chimpanzee)

Taxonomic identifier

9598 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pan

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with MORG1 By similarity.
Post-translational modification	MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 393	392	Dual specificity mitogen-activated protein kinase kinase 1		PRO_0000086367
Regions
	Domain	68 – 361	294	Protein kinase
	Nucleotide binding	74 – 82	9	ATP By similarity
	Compositional bias	262 – 307	46	Pro-rich
Sites
	Active site	190	1	Proton acceptor By similarity
	Binding site	97	1	ATP
	Site	8 – 9	2	Cleavage; by anthrax lethal factor By similarity
Amino acid modifications
	Modified residue	218	1	Phosphoserine; by RAF By similarity
	Modified residue	222	1	Phosphoserine; by RAF By similarity
	Modified residue	231	1	Phosphoserine By similarity
	Modified residue	286	1	Phosphothreonine By similarity
	Modified residue	386	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XT09-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7EE1B3208551C431	FASTA	393	43,447
	10 20 30 40 50 60 MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV 70 80 90 100 110 120 GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE 130 140 150 160 170 180 CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL 190 200 210 220 230 240 REKHKIMHRD VQPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERFQGTHY 250 260 270 280 290 300 SVQSDIWSMG LSPVEMAVGR YPIPSPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY 310 320 330 340 350 360 GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF 370 380 390 IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA AGV

References

[1]	"P. troglodytes mitogen-activated protein kinase kinase." Stockand J.D., Al-Khalili O., Spier B.J., Eaton D.C. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF143201 mRNA. Translation: AAD33901.1.
RefSeq	NP_001009071.1.
3D structure databases
SMR	Q9XT09. Positions 62-382.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9XT09.
Genome annotation databases
Ensembl	ENSPTRT00000013275; ENSPTRP00000012302; ENSPTRG00000007193; Pan troglodytes. [Genome view]
GeneID	450188.
KEGG	ptr:450188.
Organism-specific databases
CTD	450188.
Phylogenomic databases
HOVERGEN	Q9XT09.
Enzyme and pathway databases
BRENDA	2.7.12.2. 264977.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MP2K1_PANTR

Accession

Primary (citable) accession number: Q9XT09

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	January 23, 2007
Last modified:	September 1, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)