Reviewed, UniProtKB/Swiss-Prot Q9XT00 (DHB8_PIG)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 8
    EC=1.1.1.62
Alternative name(s):
    Testosterone 17-beta-dehydrogenase 8
    EC=1.1.1.63
    17-beta-hydroxysteroid dehydrogenase 8
      Short name=17-beta-HSD 8
    Protein Ke6
      Short name=Ke-6

Gene names

Name:	HSD17B8
Synonyms:	FABGL, HKE6

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Uses estradiol as its preferred substrate By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC testosterone 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Estradiol 17-beta-dehydrogenase 8		PRO_0000054600
Regions
	Nucleotide binding	13 – 37	25	NAD By similarity
Sites
	Active site	167	1	Proton acceptor By similarity
	Binding site	154	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XT00-1 [UniParc]. Last modified March 1, 2003. Version 2. Checksum: E8A357BCCD26DD1C	FASTA	259	26,622
	10 20 30 40 50 60 MASQLRLRSA LALVTGAGSG IGRAVSVRLA AEGAAVAACD LDGAAAQETV QLLGGPGSEK 70 80 90 100 110 120 GAPSGPMAAF QADVSEAETA RRLLEQVQAY FFRPPSVVVS CAGITRDEFL LRMSEDDWDK 130 140 150 160 170 180 VIAVNLKGIF LVTQAAAQAL VSSGCPGSII NISSIIGKVG NMGQTNYAAS KAGVIGLTQA 190 200 210 220 230 240 VARELGRYRI RCNSVLPGFI KTPMAQKVPQ KVLDKVVGMI PMGHLGGPPD VADVVAFLAS 250 EDSGYITGAS VEVTGGLFM

References

[1]	"Characterization of the porcine FABGL gene." Jacobs K., Mattheeuws M., Van Poucke M., Van Zeveren A., Peelman L.J. Anim. Genet. 33:220-223(2002) [PubMed: 12030927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Landrace X Pietrain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF146398 Genomic DNA. Translation: AAD44802.2.
3D structure databases
HSSP	HSSP built from PDB template 1GZ6 based on UniProtKB P97852.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9XT00.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHB8_PIG

Accession

Primary (citable) accession number: Q9XT00

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	March 1, 2003
Last modified:	November 25, 2008
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)