Reviewed, UniProtKB/Swiss-Prot Q9XSM2 (TRYT_SHEEP)

Names and origin

Protein names	Recommended name:     Tryptase-2     EC=3.4.21.59
Organism	Ovis aries (Sheep)
Taxonomic identifier	9940 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.
Subunit structure	Homotetramer By similarity.
Subcellular location	Secreted. Note: Released from the secretory granules upon mast cell activation.
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 28	10	Activation peptide By similarity		PRO_0000027488
	Chain	29 – 273	245	Tryptase-2		PRO_0000027489
Regions
	Domain	29 – 270	242	Peptidase S1
Sites
	Active site	72	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	222	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Disulfide bond	57 ↔ 73		By similarity
	Disulfide bond	153 ↔ 228		By similarity
	Disulfide bond	186 ↔ 209		By similarity
	Disulfide bond	218 ↔ 246		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XSM2-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: DE9BA79218C3E67D	FASTA	273	30,288
	10 20 30 40 50 60 MLHLLALALL LSLVSAAPAP GQALQRSGII GGKEAPGSRW PWQVSLRVRD QYWRHQCGGS 70 80 90 100 110 120 LIHPQWVLTA AHCIGPELQE PSDFRVQLRE QHLYYQDRLL PISRVIPHPH YYMVENGADI 130 140 150 160 170 180 ALLQLEEPVS ISRHVQPVTL PPASETFPPE SQCWVTGWGD VDNGRPLPPP YPLKQVKVPI 190 200 210 220 230 240 VENSVCDWKY HSGLSTDYSV PIVQEDNLCA GDGGRDSCQG DSGGPLVCKV NGTWLQAGVV 250 260 270 SWGDGCAKPN RPGIYTRITS YLDWIHQYVP QEP

References

[1]

"cDNA sequence of two sheep mast cell tryptases and the differential expression of tryptase and sheep mast cell proteinase-1 in lung, dermis and gastrointestinal tract." Pemberton A.D., McAleese S.M., Huntley J.F., Collie D.D.S., Scudamore C.L., McEuen A.R., Walls A.F., Miller H.R.P. Clin. Exp. Allergy 30:818-832(2000) [PubMed: 10848900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Abomasum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y18224 mRNA. Translation: CAB41989.1.
RefSeq	NP_001116478.1.
UniGene	Oar.751
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
SMR	Q9XSM2. Positions 29-271.
ModBase	Search...
Protein family/group databases
MEROPS	S01.118.
Genome annotation databases
GeneID	100144429.
Phylogenomic databases
HOVERGEN	Q9XSM2.
Enzyme and pathway databases
BRENDA	3.4.21.59. 271.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYT_SHEEP

Accession

Primary (citable) accession number: Q9XSM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)