ID UCHL5_BOVIN Reviewed; 328 AA. AC Q9XSJ0; Q3MHN9; DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1999, sequence version 1. DT 24-JAN-2024, entry version 141. DE RecName: Full=Ubiquitin carboxyl-terminal hydrolase isozyme L5; DE Short=UCH-L5; DE EC=3.4.19.12; DE AltName: Full=Ubiquitin C-terminal hydrolase UCH37; DE AltName: Full=Ubiquitin thioesterase L5; GN Name=UCHL5; Synonyms=UCH37; OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; OC Bovinae; Bos. OX NCBI_TaxID=9913; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=16906146; DOI=10.1038/ncb1460; RA Yao T., Song L., Xu W., DeMartino G.N., Florens L., Swanson S.K., RA Washburn M.P., Conaway R.C., Conaway J.W., Cohen R.E.; RT "Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme RT by Adrm1."; RL Nat. Cell Biol. 8:994-1002(2006). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=Crossbred X Angus; TISSUE=Liver; RG NIH - Mammalian Gene Collection (MGC) project; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Protease that specifically cleaves 'Lys-48'-linked CC polyubiquitin chains. Deubiquitinating enzyme associated with the 19S CC regulatory subunit of the 26S proteasome. Putative regulatory component CC of the INO80 complex; however is inactive in the INO80 complex and is CC activated by a transient interaction of the INO80 complex with the CC proteasome via ADRM1 (By similarity). {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- CC residue protein attached to proteins as an intracellular targeting CC signal).; EC=3.4.19.12; CC -!- ACTIVITY REGULATION: Activated by ADRM1. Inhibited by interaction with CC NFRKB (By similarity). {ECO:0000250}. CC -!- SUBUNIT: Component of the 19S (PA700) regulatory complex of the 26S CC proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 CC complex; specifically part of a complex module associated with N- CC terminus of INO80 (By similarity). {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. CC Note=Associates with the proteasome 19S subunit in the cytoplasm. CC Associates with the INO80 complex in the nucleus (By similarity). CC {ECO:0000250}. CC -!- SIMILARITY: Belongs to the peptidase C12 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF148446; AAD31533.1; -; mRNA. DR EMBL; BC105166; AAI05167.1; -; mRNA. DR RefSeq; NP_776906.2; NM_174481.3. DR AlphaFoldDB; Q9XSJ0; -. DR SMR; Q9XSJ0; -. DR STRING; 9913.ENSBTAP00000018104; -. DR MEROPS; C12.005; -. DR PaxDb; 9913-ENSBTAP00000018104; -. DR Ensembl; ENSBTAT00000018104.5; ENSBTAP00000018104.4; ENSBTAG00000013620.5. DR GeneID; 282110; -. DR KEGG; bta:282110; -. DR CTD; 51377; -. DR VEuPathDB; HostDB:ENSBTAG00000013620; -. DR VGNC; VGNC:36633; UCHL5. DR eggNOG; KOG2778; Eukaryota. DR GeneTree; ENSGT00940000155195; -. DR HOGENOM; CLU_018316_0_0_1; -. DR InParanoid; Q9XSJ0; -. DR OMA; YIQYEIQ; -. DR TreeFam; TF313976; -. DR BRENDA; 3.4.19.12; 908. DR Reactome; R-BTA-5689603; UCH proteinases. DR Proteomes; UP000009136; Chromosome 16. DR Bgee; ENSBTAG00000013620; Expressed in saliva-secreting gland and 106 other cell types or tissues. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0005829; C:cytosol; ISS:UniProtKB. DR GO; GO:0031011; C:Ino80 complex; IEA:Ensembl. DR GO; GO:0005730; C:nucleolus; IEA:Ensembl. DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl. DR GO; GO:0005634; C:nucleus; ISS:UniProtKB. DR GO; GO:0000502; C:proteasome complex; IEA:UniProtKB-KW. DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IDA:UniProtKB. DR GO; GO:0004866; F:endopeptidase inhibitor activity; IEA:Ensembl. DR GO; GO:0070628; F:proteasome binding; IDA:UniProtKB. DR GO; GO:0006338; P:chromatin remodeling; IEA:Ensembl. DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW. DR GO; GO:0048853; P:forebrain morphogenesis; IEA:Ensembl. DR GO; GO:0021670; P:lateral ventricle development; IEA:Ensembl. DR GO; GO:0030901; P:midbrain development; IEA:Ensembl. DR GO; GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Ensembl. DR GO; GO:0045739; P:positive regulation of DNA repair; IEA:Ensembl. DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IEA:Ensembl. DR GO; GO:0045880; P:positive regulation of smoothened signaling pathway; IEA:Ensembl. DR GO; GO:1904507; P:positive regulation of telomere maintenance in response to DNA damage; IEA:Ensembl. DR GO; GO:0016579; P:protein deubiquitination; ISS:UniProtKB. DR GO; GO:0051726; P:regulation of cell cycle; IEA:Ensembl. DR GO; GO:0006275; P:regulation of DNA replication; IEA:Ensembl. DR GO; GO:0060382; P:regulation of DNA strand elongation; IEA:Ensembl. DR GO; GO:0045995; P:regulation of embryonic development; IEA:Ensembl. DR GO; GO:0000723; P:telomere maintenance; IEA:Ensembl. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. DR CDD; cd02255; Peptidase_C12; 1. DR Gene3D; 1.20.58.860; -; 1. DR Gene3D; 3.40.532.10; Peptidase C12, ubiquitin carboxyl-terminal hydrolase; 1. DR InterPro; IPR038765; Papain-like_cys_pep_sf. DR InterPro; IPR001578; Peptidase_C12_UCH. DR InterPro; IPR036959; Peptidase_C12_UCH_sf. DR InterPro; IPR017390; Ubiquitinyl_hydrolase_UCH37. DR InterPro; IPR033837; UCH37. DR InterPro; IPR041507; UCH_C. DR PANTHER; PTHR10589; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1. DR PANTHER; PTHR10589:SF16; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5; 1. DR Pfam; PF01088; Peptidase_C12; 1. DR Pfam; PF18031; UCH_C; 1. DR PIRSF; PIRSF038120; Ubiquitinyl_hydrolase_UCH37; 1. DR PRINTS; PR00707; UBCTHYDRLASE. DR SUPFAM; SSF54001; Cysteine proteinases; 1. PE 2: Evidence at transcript level; KW Acetylation; Cytoplasm; DNA damage; DNA recombination; DNA repair; KW Hydrolase; Nucleus; Protease; Proteasome; Reference proteome; KW Thiol protease; Transcription; Transcription regulation; KW Ubl conjugation pathway. FT CHAIN 1..328 FT /note="Ubiquitin carboxyl-terminal hydrolase isozyme L5" FT /id="PRO_0000211065" FT REGION 312..328 FT /note="Interaction with ADRM1" FT /evidence="ECO:0000250" FT ACT_SITE 88 FT /note="Nucleophile" FT /evidence="ECO:0000250" FT ACT_SITE 164 FT /note="Proton donor" FT /evidence="ECO:0000250" FT SITE 179 FT /note="Important for enzyme activity" FT /evidence="ECO:0000250" FT MOD_RES 47 FT /note="N6-succinyllysine" FT /evidence="ECO:0000250|UniProtKB:Q9WUP7" FT MOD_RES 158 FT /note="N6-acetyllysine" FT /evidence="ECO:0000250|UniProtKB:Q9Y5K5" FT MOD_RES 288 FT /note="N6-succinyllysine" FT /evidence="ECO:0000250|UniProtKB:Q9WUP7" FT CONFLICT 306 FT /note="Q -> K (in Ref. 2; AAI05167)" FT /evidence="ECO:0000305" SQ SEQUENCE 328 AA; 37432 MW; D6EFE9C71900CBB0 CRC64; MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDAKTAAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEPMDTD QGSNMLSAIQ SEVAKNQMLI EEEVQKLKRY KIENIRRKHN YLPFIMELLK TLAEHQQLIP LVEKAKEKQN AKKAQETK //