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Q9XSJ0 (UCHL5_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5

Short name=UCH-L5
EC=3.4.19.12
Alternative name(s):
Ubiquitin C-terminal hydrolase UCH37
Ubiquitin thioesterase L5
Gene names
Name:UCHL5
Synonyms:UCH37
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation

Activated by ADRM1. Inhibited by interaction with NFRKB By similarity.

Subunit structure

Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus By similarity.

Sequence similarities

Belongs to the peptidase C12 family.

Ontologies

Keywords
   Biological processDNA damage
DNA recombination
DNA repair
Transcription
Transcription regulation
Ubl conjugation pathway
   Cellular componentCytoplasm
Nucleus
Proteasome
   Molecular functionHydrolase
Protease
Thiol protease
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA recombination

Inferred from electronic annotation. Source: UniProtKB-KW

DNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

forebrain morphogenesis

Inferred from electronic annotation. Source: Ensembl

lateral ventricle development

Inferred from electronic annotation. Source: Ensembl

midbrain development

Inferred from electronic annotation. Source: Ensembl

protein deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of proteasomal protein catabolic process

Inferred from electronic annotation. Source: Ensembl

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentIno80 complex

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

proteasome complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionendopeptidase inhibitor activity

Inferred from electronic annotation. Source: Ensembl

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

proteasome binding

Inferred from direct assay PubMed 18162577. Source: UniProtKB

ubiquitin thiolesterase activity

Inferred from direct assay PubMed 18162577. Source: UniProtKB

ubiquitin-specific protease activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Ubiquitin carboxyl-terminal hydrolase isozyme L5
PRO_0000211065

Regions

Region312 – 32817Interaction with ADRM1 By similarity

Sites

Active site881Nucleophile By similarity
Active site1641Proton donor By similarity
Site1791Important for enzyme activity By similarity

Amino acid modifications

Modified residue471N6-succinyllysine By similarity
Modified residue1581N6-acetyllysine By similarity
Modified residue2881N6-succinyllysine By similarity

Experimental info

Sequence conflict3061Q → K in AAI05167. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9XSJ0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: D6EFE9C71900CBB0

FASTA32837,432
        10         20         30         40         50         60 
MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP 

        70         80         90        100        110        120 
GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS 

       130        140        150        160        170        180 
FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDAKTAAKEE DAFHFVSYVP VNGRLYELDG 

       190        200        210        220        230        240 
LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR 

       250        260        270        280        290        300 
QLAEEPMDTD QGSNMLSAIQ SEVAKNQMLI EEEVQKLKRY KIENIRRKHN YLPFIMELLK 

       310        320 
TLAEHQQLIP LVEKAKEKQN AKKAQETK 

« Hide

References

« Hide 'large scale' references
[1]"Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1."
Yao T., Song L., Xu W., DeMartino G.N., Florens L., Swanson S.K., Washburn M.P., Conaway R.C., Conaway J.W., Cohen R.E.
Nat. Cell Biol. 8:994-1002(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF148446 mRNA. Translation: AAD31533.1.
BC105166 mRNA. Translation: AAI05167.1.
RefSeqNP_776906.2. NM_174481.3.
UniGeneBt.4689.

3D structure databases

ProteinModelPortalQ9XSJ0.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC12.005.

Proteomic databases

PRIDEQ9XSJ0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000018104; ENSBTAP00000018104; ENSBTAG00000013620.
GeneID282110.
KEGGbta:282110.

Organism-specific databases

CTD51377.

Phylogenomic databases

eggNOGNOG321645.
GeneTreeENSGT00510000046560.
HOGENOMHOG000203918.
HOVERGENHBG056021.
InParanoidQ9XSJ0.
KOK05610.
OMADEDAFHF.
OrthoDBEOG7M3J11.
TreeFamTF313976.

Enzyme and pathway databases

BRENDA3.4.19.12. 908.

Family and domain databases

Gene3D3.40.532.10. 1 hit.
InterProIPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view]
PANTHERPTHR10589. PTHR10589. 1 hit.
PfamPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PIRSFPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSPR00707. UBCTHYDRLASE.
ProtoNetSearch...

Other

NextBio20805949.

Entry information

Entry nameUCHL5_BOVIN
AccessionPrimary (citable) accession number: Q9XSJ0
Secondary accession number(s): Q3MHN9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1999
Last modified: March 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries