Q9XSJ0 (UCHL5_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5
Short name=UCH-L5
EC=3.4.19.12

Alternative name(s):
Ubiquitin C-terminal hydrolase UCH37
Ubiquitin thioesterase L5

Gene names

Name:	UCHL5
Synonyms:	UCH37

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Enzyme regulation	Activated by ADRM1. Inhibited by interaction with NFRKB By similarity.
Subunit structure	Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus By similarity.
Sequence similarities	Belongs to the peptidase C12 family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Thiol protease
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW forebrain morphogenesis Inferred from electronic annotation. Source: Compara lateral ventricle development Inferred from electronic annotation. Source: Compara midbrain development Inferred from electronic annotation. Source: Compara protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB regulation of proteasomal protein catabolic process Inferred from electronic annotation. Source: Compara regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	Ino80 complex Inferred from electronic annotation. Source: Compara cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB proteasome complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	endopeptidase inhibitor activity Inferred from electronic annotation. Source: Compara omega peptidase activity Inferred from electronic annotation. Source: InterPro proteasome binding Inferred from direct assay PubMed 18162577. Source: UniProtKB ubiquitin thiolesterase activity Inferred from direct assay PubMed 18162577. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Ubiquitin carboxyl-terminal hydrolase isozyme L5		PRO_0000211065
Regions
	Region	312 – 328	17	Interaction with ADRM1 By similarity
Sites
	Active site	88	1	Nucleophile By similarity
	Active site	164	1	Proton donor By similarity
	Site	179	1	Important for enzyme activity By similarity
Amino acid modifications
	Modified residue	158	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	306	1	Q → K in AAI05167. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9XSJ0 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: D6EFE9C71900CBB0	FASTA	328	37,432
	10 20 30 40 50 60 MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP 70 80 90 100 110 120 GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS 130 140 150 160 170 180 FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDAKTAAKEE DAFHFVSYVP VNGRLYELDG 190 200 210 220 230 240 LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR 250 260 270 280 290 300 QLAEEPMDTD QGSNMLSAIQ SEVAKNQMLI EEEVQKLKRY KIENIRRKHN YLPFIMELLK 310 320 TLAEHQQLIP LVEKAKEKQN AKKAQETK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1." Yao T., Song L., Xu W., DeMartino G.N., Florens L., Swanson S.K., Washburn M.P., Conaway R.C., Conaway J.W., Cohen R.E. Nat. Cell Biol. 8:994-1002(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF148446 mRNA. Translation: AAD31533.1. BC105166 mRNA. Translation: AAI05167.1.
IPI	IPI00694182.
RefSeq	NP_776906.2. NM_174481.3.
UniGene	Bt.4689.
3D structure databases
ProteinModelPortal	Q9XSJ0.
ModBase	Search...
Protein family/group databases
MEROPS	C12.005.
Proteomic databases
PRIDE	Q9XSJ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000018104; ENSBTAP00000018104; ENSBTAG00000013620.
GeneID	282110.
KEGG	bta:282110.
Organism-specific databases
CTD	51377.
Phylogenomic databases
eggNOG	NOG321645.
GeneTree	ENSGT00510000046560.
HOGENOM	HOG000203918.
HOVERGEN	HBG056021.
InParanoid	Q9XSJ0.
KO	K05610.
OMA	QMFEFDT.
OrthoDB	EOG4RBQK0.
Enzyme and pathway databases
BRENDA	3.4.19.12. 908.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. IPR017390. Ubiquitinyl_hydrolase_UCH37. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PIRSF	PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTS	PR00707. UBCTHYDRLASE.
PROSITE	PS00140. UCH_1. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805949.

Entry information

Entry name

UCHL5_BOVIN

Accession

Primary (citable) accession number: Q9XSJ0
Secondary accession number(s): Q3MHN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program