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Q9XSJ0

- UCHL5_BOVIN

UniProt

Q9XSJ0 - UCHL5_BOVIN

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Protein
Ubiquitin carboxyl-terminal hydrolase isozyme L5
Gene
UCHL5, UCH37
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Activated by ADRM1. Inhibited by interaction with NFRKB By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Nucleophile By similarity
Active sitei164 – 1641Proton donor By similarity
Sitei179 – 1791Important for enzyme activity By similarity

GO - Molecular functioni

  1. endopeptidase inhibitor activity Source: Ensembl
  2. omega peptidase activity Source: InterPro
  3. proteasome binding Source: UniProtKB
  4. ubiquitin thiolesterase activity Source: UniProtKB
  5. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
  3. forebrain morphogenesis Source: Ensembl
  4. lateral ventricle development Source: Ensembl
  5. midbrain development Source: Ensembl
  6. protein deubiquitination Source: UniProtKB
  7. regulation of proteasomal protein catabolic process Source: Ensembl
  8. regulation of transcription, DNA-templated Source: UniProtKB-KW
  9. transcription, DNA-templated Source: UniProtKB-KW
  10. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

BRENDAi3.4.19.12. 908.
ReactomeiREACT_225632. Downregulation of TGF-beta receptor signaling.

Protein family/group databases

MEROPSiC12.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5 (EC:3.4.19.12)
Short name:
UCH-L5
Alternative name(s):
Ubiquitin C-terminal hydrolase UCH37
Ubiquitin thioesterase L5
Gene namesi
Name:UCHL5
Synonyms:UCH37
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 16

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus By similarity.

GO - Cellular componenti

  1. Ino80 complex Source: Ensembl
  2. cytosol Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. proteasome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Ubiquitin carboxyl-terminal hydrolase isozyme L5
PRO_0000211065Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 471N6-succinyllysine By similarity
Modified residuei158 – 1581N6-acetyllysine By similarity
Modified residuei288 – 2881N6-succinyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ9XSJ0.

Interactioni

Subunit structurei

Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9XSJ0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni312 – 32817Interaction with ADRM1 By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C12 family.

Phylogenomic databases

eggNOGiNOG321645.
GeneTreeiENSGT00510000046560.
HOGENOMiHOG000203918.
HOVERGENiHBG056021.
InParanoidiQ9XSJ0.
KOiK05610.
OMAiDEDAFHF.
OrthoDBiEOG7M3J11.
TreeFamiTF313976.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.

Sequencei

Sequence statusi: Complete.

Q9XSJ0-1 [UniParc]FASTAAdd to Basket

« Hide

MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH    50
GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC 100
THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE 150
FDAKTAAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV 200
RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEPMDTD 250
QGSNMLSAIQ SEVAKNQMLI EEEVQKLKRY KIENIRRKHN YLPFIMELLK 300
TLAEHQQLIP LVEKAKEKQN AKKAQETK 328
Length:328
Mass (Da):37,432
Last modified:November 1, 1999 - v1
Checksum:iD6EFE9C71900CBB0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti306 – 3061Q → K in AAI05167. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148446 mRNA. Translation: AAD31533.1.
BC105166 mRNA. Translation: AAI05167.1.
RefSeqiNP_776906.2. NM_174481.3.
UniGeneiBt.4689.

Genome annotation databases

EnsembliENSBTAT00000018104; ENSBTAP00000018104; ENSBTAG00000013620.
GeneIDi282110.
KEGGibta:282110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148446 mRNA. Translation: AAD31533.1 .
BC105166 mRNA. Translation: AAI05167.1 .
RefSeqi NP_776906.2. NM_174481.3.
UniGenei Bt.4689.

3D structure databases

ProteinModelPortali Q9XSJ0.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C12.005.

Proteomic databases

PRIDEi Q9XSJ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000018104 ; ENSBTAP00000018104 ; ENSBTAG00000013620 .
GeneIDi 282110.
KEGGi bta:282110.

Organism-specific databases

CTDi 51377.

Phylogenomic databases

eggNOGi NOG321645.
GeneTreei ENSGT00510000046560.
HOGENOMi HOG000203918.
HOVERGENi HBG056021.
InParanoidi Q9XSJ0.
KOi K05610.
OMAi DEDAFHF.
OrthoDBi EOG7M3J11.
TreeFami TF313976.

Enzyme and pathway databases

BRENDAi 3.4.19.12. 908.
Reactomei REACT_225632. Downregulation of TGF-beta receptor signaling.

Miscellaneous databases

NextBioi 20805949.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PIRSFi PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSi PR00707. UBCTHYDRLASE.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1."
    Yao T., Song L., Xu W., DeMartino G.N., Florens L., Swanson S.K., Washburn M.P., Conaway R.C., Conaway J.W., Cohen R.E.
    Nat. Cell Biol. 8:994-1002(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiUCHL5_BOVIN
AccessioniPrimary (citable) accession number: Q9XSJ0
Secondary accession number(s): Q3MHN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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