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Q9XSJ0

- UCHL5_BOVIN

UniProt

Q9XSJ0 - UCHL5_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Gene

UCHL5

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    Activated by ADRM1. Inhibited by interaction with NFRKB By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881NucleophileBy similarity
    Active sitei164 – 1641Proton donorBy similarity
    Sitei179 – 1791Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. endopeptidase inhibitor activity Source: Ensembl
    2. omega peptidase activity Source: InterPro
    3. proteasome binding Source: UniProtKB
    4. ubiquitin-specific protease activity Source: UniProtKB
    5. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW
    3. forebrain morphogenesis Source: Ensembl
    4. lateral ventricle development Source: Ensembl
    5. midbrain development Source: Ensembl
    6. protein deubiquitination Source: UniProtKB
    7. regulation of proteasomal protein catabolic process Source: Ensembl
    8. regulation of transcription, DNA-templated Source: UniProtKB-KW
    9. transcription, DNA-templated Source: UniProtKB-KW
    10. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation, Ubl conjugation pathway

    Enzyme and pathway databases

    BRENDAi3.4.19.12. 908.
    ReactomeiREACT_225632. Downregulation of TGF-beta receptor signaling.

    Protein family/group databases

    MEROPSiC12.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L5 (EC:3.4.19.12)
    Short name:
    UCH-L5
    Alternative name(s):
    Ubiquitin C-terminal hydrolase UCH37
    Ubiquitin thioesterase L5
    Gene namesi
    Name:UCHL5
    Synonyms:UCH37
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 16

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. Ino80 complex Source: Ensembl
    3. nucleus Source: UniProtKB
    4. proteasome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Ubiquitin carboxyl-terminal hydrolase isozyme L5PRO_0000211065Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei47 – 471N6-succinyllysineBy similarity
    Modified residuei158 – 1581N6-acetyllysineBy similarity
    Modified residuei288 – 2881N6-succinyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ9XSJ0.

    Interactioni

    Subunit structurei

    Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XSJ0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni312 – 32817Interaction with ADRM1By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG321645.
    GeneTreeiENSGT00510000046560.
    HOGENOMiHOG000203918.
    HOVERGENiHBG056021.
    InParanoidiQ9XSJ0.
    KOiK05610.
    OMAiDEDAFHF.
    OrthoDBiEOG7M3J11.
    TreeFamiTF313976.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    IPR017390. Ubiquitinyl_hydrolase_UCH37.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
    PRINTSiPR00707. UBCTHYDRLASE.

    Sequencei

    Sequence statusi: Complete.

    Q9XSJ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH    50
    GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC 100
    THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE 150
    FDAKTAAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV 200
    RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEPMDTD 250
    QGSNMLSAIQ SEVAKNQMLI EEEVQKLKRY KIENIRRKHN YLPFIMELLK 300
    TLAEHQQLIP LVEKAKEKQN AKKAQETK 328
    Length:328
    Mass (Da):37,432
    Last modified:November 1, 1999 - v1
    Checksum:iD6EFE9C71900CBB0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti306 – 3061Q → K in AAI05167. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148446 mRNA. Translation: AAD31533.1.
    BC105166 mRNA. Translation: AAI05167.1.
    RefSeqiNP_776906.2. NM_174481.3.
    UniGeneiBt.4689.

    Genome annotation databases

    EnsembliENSBTAT00000018104; ENSBTAP00000018104; ENSBTAG00000013620.
    GeneIDi282110.
    KEGGibta:282110.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148446 mRNA. Translation: AAD31533.1 .
    BC105166 mRNA. Translation: AAI05167.1 .
    RefSeqi NP_776906.2. NM_174481.3.
    UniGenei Bt.4689.

    3D structure databases

    ProteinModelPortali Q9XSJ0.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C12.005.

    Proteomic databases

    PRIDEi Q9XSJ0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000018104 ; ENSBTAP00000018104 ; ENSBTAG00000013620 .
    GeneIDi 282110.
    KEGGi bta:282110.

    Organism-specific databases

    CTDi 51377.

    Phylogenomic databases

    eggNOGi NOG321645.
    GeneTreei ENSGT00510000046560.
    HOGENOMi HOG000203918.
    HOVERGENi HBG056021.
    InParanoidi Q9XSJ0.
    KOi K05610.
    OMAi DEDAFHF.
    OrthoDBi EOG7M3J11.
    TreeFami TF313976.

    Enzyme and pathway databases

    BRENDAi 3.4.19.12. 908.
    Reactomei REACT_225632. Downregulation of TGF-beta receptor signaling.

    Miscellaneous databases

    NextBioi 20805949.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    IPR017390. Ubiquitinyl_hydrolase_UCH37.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
    PRINTSi PR00707. UBCTHYDRLASE.
    ProtoNeti Search...

    Publicationsi

    1. "Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1."
      Yao T., Song L., Xu W., DeMartino G.N., Florens L., Swanson S.K., Washburn M.P., Conaway R.C., Conaway J.W., Cohen R.E.
      Nat. Cell Biol. 8:994-1002(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiUCHL5_BOVIN
    AccessioniPrimary (citable) accession number: Q9XSJ0
    Secondary accession number(s): Q3MHN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3