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Q9XSG3

- IDHC_BOVIN

UniProt

Q9XSG3 - IDHC_BOVIN

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Protein

Isocitrate dehydrogenase [NADP] cytoplasmic

Gene

IDH1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.1 Publication

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateBy similarity
Binding sitei82 – 821NADPBy similarity
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei132 – 1321SubstrateBy similarity
Sitei139 – 1391Critical for catalysisBy similarity
Sitei212 – 2121Critical for catalysisBy similarity
Metal bindingi252 – 2521Magnesium or manganeseBy similarity
Binding sitei260 – 2601NADPBy similarity
Metal bindingi275 – 2751Magnesium or manganeseBy similarity
Binding sitei328 – 3281NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 773NADPBy similarity
Nucleotide bindingi310 – 3156NADPBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. NAD binding Source: InterPro

GO - Biological processi

  1. 2-oxoglutarate metabolic process Source: UniProtKB
  2. glutathione metabolic process Source: AgBase
  3. glyoxylate cycle Source: UniProtKB-KW
  4. isocitrate metabolic process Source: UniProtKB
  5. response to oxidative stress Source: AgBase
  6. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_210137. NADPH regeneration.
REACT_212129. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:IDH1
Synonyms:ICDH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 2

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: AgBase
  2. cytosol Source: AgBase
  3. extracellular vesicular exosome Source: Ensembl
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 414413Isocitrate dehydrogenase [NADP] cytoplasmicPRO_0000236188Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei81 – 811N6-acetyllysineBy similarity
Modified residuei126 – 1261N6-succinyllysineBy similarity
Modified residuei224 – 2241N6-acetyllysineBy similarity
Modified residuei233 – 2331N6-acetyllysineBy similarity
Modified residuei321 – 3211N6-acetyllysineBy similarity
Modified residuei400 – 4001N6-succinyllysineBy similarity

Post-translational modificationi

Acetylation at Lys-374 dramatically reduces catalytic activity.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9XSG3.
PRIDEiQ9XSG3.

Expressioni

Tissue specificityi

Expressed preferentially in corneal epithelium. Constitute approximately 13% of the total soluble bovine corneal epithelial proteins.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9XSG3.
SMRiQ9XSG3. Positions 1-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 1007Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0538.
GeneTreeiENSGT00390000012547.
HOGENOMiHOG000019858.
HOVERGENiHBG006119.
InParanoidiQ9XSG3.
KOiK00031.
OrthoDBiEOG7QNVKS.
TreeFamiTF300428.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XSG3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQKIQGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD
60 70 80 90 100
ATNDQVTKDA AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG
160 170 180 190 200
KVEISYTPSD GSPKTVYLVH NFTESGGVAM GMYNQDKSIE DFAHSSFQMA
210 220 230 240 250
LSKNWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSEFE AQNIWYEHRL
260 270 280 290 300
IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG
310 320 330 340 350
KTVEAEAAHG TVTRHYRMYQ KGQETLTNPI ASIFAWTRGL AHRAKLDNNK
360 370 380 390 400
ELSFFAKALE EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK
410
LGENLQLKLA QAKL
Length:414
Mass (Da):46,785
Last modified:November 1, 1999 - v1
Checksum:iA4E8B08679D32476
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti326 – 3261L → S in AAI03369. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136009 mRNA. Translation: AAD34457.1.
BC103368 mRNA. Translation: AAI03369.1.
RefSeqiNP_851355.2. NM_181012.3.
XP_005202754.1. XM_005202697.1.
UniGeneiBt.13324.

Genome annotation databases

EnsembliENSBTAT00000027348; ENSBTAP00000027348; ENSBTAG00000020527.
GeneIDi281235.
KEGGibta:281235.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136009 mRNA. Translation: AAD34457.1 .
BC103368 mRNA. Translation: AAI03369.1 .
RefSeqi NP_851355.2. NM_181012.3.
XP_005202754.1. XM_005202697.1.
UniGenei Bt.13324.

3D structure databases

ProteinModelPortali Q9XSG3.
SMRi Q9XSG3. Positions 1-414.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9XSG3.
PRIDEi Q9XSG3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000027348 ; ENSBTAP00000027348 ; ENSBTAG00000020527 .
GeneIDi 281235.
KEGGi bta:281235.

Organism-specific databases

CTDi 3417.

Phylogenomic databases

eggNOGi COG0538.
GeneTreei ENSGT00390000012547.
HOGENOMi HOG000019858.
HOVERGENi HBG006119.
InParanoidi Q9XSG3.
KOi K00031.
OrthoDBi EOG7QNVKS.
TreeFami TF300428.

Enzyme and pathway databases

Reactomei REACT_210137. NADPH regeneration.
REACT_212129. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.

Miscellaneous databases

NextBioi 20805281.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a cytosolic NADP+-dependent isocitrate dehydrogenase that is preferentially expressed in bovine corneal epithelium. A corneal epithelial crystallin."
    Sun L., Sun T.T., Lavker R.M.
    J. Biol. Chem. 274:17334-17341(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiIDHC_BOVIN
AccessioniPrimary (citable) accession number: Q9XSG3
Secondary accession number(s): Q3SYV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3