Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9XSG3 (IDHC_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic

Short name=IDH
EC=1.1.1.42
Alternative name(s):
Cytosolic NADP-isocitrate dehydrogenase
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene names
Name:IDH1
Synonyms:ICDH
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency. Ref.1

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Expressed preferentially in corneal epithelium. Constitute approximately 13% of the total soluble bovine corneal epithelial proteins. Ref.1

Post-translational modification

Acetylation at Lys-374 dramatically reduces catalytic activity By similarity.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 414413Isocitrate dehydrogenase [NADP] cytoplasmic
PRO_0000236188

Regions

Nucleotide binding75 – 773NADP By similarity
Nucleotide binding310 – 3156NADP By similarity
Region94 – 1007Substrate binding By similarity

Sites

Metal binding2521Magnesium or manganese By similarity
Metal binding2751Magnesium or manganese By similarity
Binding site771Substrate By similarity
Binding site821NADP By similarity
Binding site1091Substrate By similarity
Binding site1321Substrate By similarity
Binding site2601NADP By similarity
Binding site3281NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1391Critical for catalysis By similarity
Site2121Critical for catalysis By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue811N6-acetyllysine By similarity
Modified residue1261N6-succinyllysine By similarity
Modified residue2241N6-acetyllysine By similarity
Modified residue2331N6-acetyllysine By similarity
Modified residue3211N6-acetyllysine By similarity
Modified residue4001N6-succinyllysine By similarity

Experimental info

Sequence conflict3261L → S in AAI03369. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9XSG3 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: A4E8B08679D32476

FASTA41446,785
        10         20         30         40         50         60 
MSQKIQGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA 

        70         80         90        100        110        120 
AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL 

       130        140        150        160        170        180 
VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEISYTPSD GSPKTVYLVH NFTESGGVAM 

       190        200        210        220        230        240 
GMYNQDKSIE DFAHSSFQMA LSKNWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSEFE 

       250        260        270        280        290        300 
AQNIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG 

       310        320        330        340        350        360 
KTVEAEAAHG TVTRHYRMYQ KGQETLTNPI ASIFAWTRGL AHRAKLDNNK ELSFFAKALE 

       370        380        390        400        410 
EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLQLKLA QAKL 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a cytosolic NADP+-dependent isocitrate dehydrogenase that is preferentially expressed in bovine corneal epithelium. A corneal epithelial crystallin."
Sun L., Sun T.T., Lavker R.M.
J. Biol. Chem. 274:17334-17341(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Ileum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF136009 mRNA. Translation: AAD34457.1.
BC103368 mRNA. Translation: AAI03369.1.
RefSeqNP_851355.2. NM_181012.3.
XP_005202754.1. XM_005202697.1.
UniGeneBt.13324.

3D structure databases

ProteinModelPortalQ9XSG3.
SMRQ9XSG3. Positions 1-414.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9XSG3.
PRIDEQ9XSG3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000027348; ENSBTAP00000027348; ENSBTAG00000020527.
GeneID281235.
KEGGbta:281235.

Organism-specific databases

CTD3417.

Phylogenomic databases

eggNOGCOG0538.
GeneTreeENSGT00390000012547.
HOGENOMHOG000019858.
HOVERGENHBG006119.
InParanoidQ9XSG3.
KOK00031.
OrthoDBEOG7QNVKS.
TreeFamTF300428.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805281.

Entry information

Entry nameIDHC_BOVIN
AccessionPrimary (citable) accession number: Q9XSG3
Secondary accession number(s): Q3SYV2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families