Isocitrate dehydrogenase [NADP] cytoplasmic

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Reviewed, UniProtKB/Swiss-Prot Q9XSG3 (IDHC_BOVIN)

Last modified February 9, 2010. Version 52. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP] cytoplasmic
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Cytosolic NADP-isocitrate dehydrogenase
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:	IDH1
Synonyms:	ICDH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency. Ref.1
Catalytic activity	Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH. Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed preferentially in corneal epithelium. Constitute approximately 13% of the total soluble bovine corneal epithelial proteins. Ref.1
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

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Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	glutathione metabolic process Inferred from sequence or structural similarity. Source: AgBase glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from sequence or structural similarity. Source: AgBase tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from sequence or structural similarity. Source: AgBase
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from sequence or structural similarity. Source: AgBase magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 414

414

Isocitrate dehydrogenase [NADP] cytoplasmic

PRO_0000236188

Regions

Nucleotide binding

75 – 77

NADP By similarity

Nucleotide binding

310 – 315

NADP By similarity

Region

94 – 100

Substrate binding By similarity

Sites

Metal binding

252

Magnesium or manganese By similarity

Metal binding

275

Magnesium or manganese By similarity

Binding site

Substrate By similarity

Binding site

NADP By similarity

Binding site

109

Substrate By similarity

Binding site

132

Substrate By similarity

Binding site

260

NADP By similarity

Binding site

328

NADP; via amide nitrogen and carbonyl oxygen By similarity

Site

139

Critical for catalysis By similarity

Site

212

Critical for catalysis By similarity

Amino acid modifications

Modified residue

N6-acetyllysine By similarity

Modified residue

224

N6-acetyllysine By similarity

Modified residue

321

N6-acetyllysine By similarity

Experimental info

Sequence conflict

326

L → S in AAI03369. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9XSG3-1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: A4E8B08679D32476
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FASTA

414

46,785

        10         20         30         40         50         60 
MSQKIQGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA 

        70         80         90        100        110        120 
AEAIKKYNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL 

       130        140        150        160        170        180 
VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEISYTPSD GSPKTVYLVH NFTESGGVAM 

       190        200        210        220        230        240 
GMYNQDKSIE DFAHSSFQMA LSKNWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSEFE 

       250        260        270        280        290        300 
AQNIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG 

       310        320        330        340        350        360 
KTVEAEAAHG TVTRHYRMYQ KGQETLTNPI ASIFAWTRGL AHRAKLDNNK ELSFFAKALE 

       370        380        390        400        410 
EVCIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLQLKLA QAKL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a cytosolic NADP+-dependent isocitrate dehydrogenase that is preferentially expressed in bovine corneal epithelium. A corneal epithelial crystallin." Sun L., Sun T.T., Lavker R.M. J. Biol. Chem. 274:17334-17341(1999) [PubMed: 10358094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AF136009 mRNA. Translation: AAD34457.1. BC103368 mRNA. Translation: AAI03369.1.
IPI	IPI00702781.
RefSeq	NP_851355.2.
UniGene	Bt.13324
3D structure databases
SMR	Q9XSG3. Positions 1-414.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9XSG3.
Genome annotation databases
Ensembl	ENSBTAT00000027348; ENSBTAP00000027348; ENSBTAG00000020527; Bos taurus. [Genome view]
GeneID	281235.
KEGG	bta:281235.
Organism-specific databases
CTD	281235.
Phylogenomic databases
HOVERGEN	Q9XSG3.
InParanoid	Q9XSG3.
PhylomeDB	Q9XSG3.
Enzyme and pathway databases
BRENDA	1.1.1.42. 251.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

IDHC_BOVIN

Accession

Primary (citable) accession number: Q9XSG3
Secondary accession number(s): Q3SYV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents