Reviewed, UniProtKB/Swiss-Prot Q9XS79 (HEM1_DELLE)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase, nonspecific, mitochondrial
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthetase
      Short name=ALAS-H

Gene names

Name:	ALAS1
Synonyms:	ALS1

Organism

Delphinapterus leucas (Beluga whale)

Taxonomic identifier

9749 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Cetacea › Odontoceti › Monodontidae › Delphinapterus

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Miscellaneous	There are two delta-ALA synthetase in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 56	56	Mitochondrion By similarity
	Chain	57 – 640	584	5-aminolevulinate synthase, nonspecific, mitochondrial		PRO_0000001229
Amino acid modifications
	Modified residue	445	1	N6-(pyridoxal phosphate)lysine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q9XS79-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 357BC41CE8EDBA91	FASTA	640	70,620
	10 20 30 40 50 60 METVVRRCPF LSRVPQAFLQ KAGKSLLFYA QNCPKMMEVG AKPAPRALSA SAVLCQQVKE 70 80 90 100 110 120 TPPANEKDKT AKAKVQQAPD GSQQTPDGTQ LPSGHPSLAA SQGTASKCPF LAAQMSQGGS 130 140 150 160 170 180 SVFCKASLEL QEDVQEMHAV REEVAQTSVN PSVISVKTDG GELSGLLKNF QDIMRKQRPE 190 200 210 220 230 240 GVSHLLQDNL PKSVSTFQYD HFFEKKIDEK KNDHTYRVFK TVNRRAQFFP MADDYSDSLV 250 260 270 280 290 300 TKKQVSVWCS NDYLGMSCHP RVCGAVMDTL KQHGTGAGGT RNISGTSKFH VDLEQELADL 310 320 330 340 350 360 HGKDAALLFS SCFVANDSTL FTLAKMMPGC EIYSDSGNHA SMIQGIWNSR VPKYIYRHND 370 380 390 400 410 420 VDHLRELLQR SDPAVPKIVA FETVHSMDGA VCPLEELCDV AHEFGAITFV DEVHAVGLYG 430 440 450 460 470 480 AQGGGIGDRD GVMPKMDIIS GTLGKAFGCV GGYIASTSSL IDTIRSYAAG FIFTTSLPPM 490 500 510 520 530 540 LLAGALESVR ILKSTEGRVL RRQHQRNVKL MRQMLMDASL PVVHCPSHII PVRVADAAKN 550 560 570 580 590 600 TEVCNELMSR HNIYVQAINY PTVRRGEELL RIAPTPHHTP QMMNYFVENL LATWKRVGLE 610 620 630 640 LKPHSSAECN FCRRPLHFEV MSEREKSYFF GMSKLVSAQD

References

[1]

"Comparison of the beluga whale (Delphinapterus leucas) expressed genes for 5-aminolevulinate synthase with those in other vertebrates." Kreiling J.A., Duncan R., Faggart M.A., Cornell N.W. Comp. Biochem. Physiol. 123B:163-174(1999) [PubMed: 10425720] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF073337 mRNA. Translation: AAD20806.1.
3D structure databases
HSSP	HSSP built from PDB template 1BS0 based on UniProtKB P12998.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9XS79.
Enzyme and pathway databases
BRENDA	2.3.1.37. 191613.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_DELLE

Accession

Primary (citable) accession number: Q9XS79

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	November 1, 1999
Last modified:	September 22, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)