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Q9XS65

- PTGDS_CANFA

UniProt

Q9XS65 - PTGDS_CANFA

Protein

Prostaglandin-H2 D-isomerase

Gene

PTGDS

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system By similarity.By similarity

    Catalytic activityi

    (5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651NucleophileBy similarity

    GO - Molecular functioni

    1. prostaglandin-D synthase activity Source: UniProtKB
    2. retinoid binding Source: UniProtKB
    3. small molecule binding Source: InterPro
    4. transporter activity Source: UniProtKB

    GO - Biological processi

    1. prostaglandin biosynthetic process Source: UniProtKB
    2. regulation of circadian sleep/wake cycle, sleep Source: UniProtKB
    3. transport Source: UniProtKB

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism, Transport

    Enzyme and pathway databases

    ReactomeiREACT_174506. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prostaglandin-H2 D-isomerase (EC:5.3.99.2)
    Alternative name(s):
    Glutathione-independent PGD synthase
    Lipocalin-type prostaglandin-D synthase
    Prostaglandin-D2 synthase
    Short name:
    PGD2 synthase
    Short name:
    PGDS
    Short name:
    PGDS2
    Gene namesi
    Name:PTGDS
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Chromosome 9

    Subcellular locationi

    Rough endoplasmic reticulum By similarity. Nucleus membrane By similarity. Golgi apparatus By similarity. Cytoplasmperinuclear region By similarity. Secreted By similarity
    Note: Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted By similarity.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular space Source: UniProtKB
    3. Golgi apparatus Source: UniProtKB
    4. nuclear membrane Source: UniProtKB-SubCell
    5. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    6. rough endoplasmic reticulum Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424By similarityAdd
    BLAST
    Chaini25 – 191167Prostaglandin-H2 D-isomerasePRO_0000017941Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei25 – 251Pyrrolidone carboxylic acidBy similarity
    Glycosylationi51 – 511N-linked (GlcNAc...)By similarity
    Glycosylationi78 – 781N-linked (GlcNAc...)By similarity
    Disulfide bondi89 ↔ 186By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000028880.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XS65.
    SMRiQ9XS65. Positions 24-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.By similarity

    Sequence similaritiesi

    Belongs to the calycin superfamily. Lipocalin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG45731.
    GeneTreeiENSGT00620000088005.
    HOGENOMiHOG000231660.
    HOVERGENiHBG106490.
    InParanoidiQ9XS65.
    KOiK01830.
    OrthoDBiEOG78PVBH.

    Family and domain databases

    Gene3Di2.40.128.20. 1 hit.
    InterProiIPR012674. Calycin.
    IPR011038. Calycin-like.
    IPR002345. Lipocalin.
    IPR000566. Lipocln_cytosolic_FA-bd_dom.
    IPR002972. PstgldnD_synth.
    [Graphical view]
    PfamiPF00061. Lipocalin. 1 hit.
    [Graphical view]
    PRINTSiPR00179. LIPOCALIN.
    PR01254. PGNDSYNTHASE.
    SUPFAMiSSF50814. SSF50814. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9XS65-1 [UniParc]FASTAAdd to Basket

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    MGALCTLWLG LVLLGVLGAL QTSAQAQVSL QPNFQQDKFL GRWFTSGLAS    50
    NSSWFREKKN VLSMCMSVVA PTADGGLNLT STFLRKDQCE TRTLLLRPAG 100
    TPGCYSYTSP HWGSTHDVWV VATNYEEYAL LYTAGSKGLG QDFHMATLYS 150
    RTQTPKAEIK EKFSTFAKTQ GFTEDAIVFL PQTDKCMEEN K 191
    Length:191
    Mass (Da):21,142
    Last modified:November 1, 1999 - v1
    Checksum:i05DD17A63001C256
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti9 – 91L → R.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026988 mRNA. Translation: BAA77690.1.
    RefSeqiNP_001003131.1. NM_001003131.1.
    UniGeneiCfa.39564.

    Genome annotation databases

    EnsembliENSCAFT00000031047; ENSCAFP00000028880; ENSCAFG00000019533.
    GeneIDi403740.
    KEGGicfa:403740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026988 mRNA. Translation: BAA77690.1 .
    RefSeqi NP_001003131.1. NM_001003131.1.
    UniGenei Cfa.39564.

    3D structure databases

    ProteinModelPortali Q9XS65.
    SMRi Q9XS65. Positions 24-189.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000028880.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCAFT00000031047 ; ENSCAFP00000028880 ; ENSCAFG00000019533 .
    GeneIDi 403740.
    KEGGi cfa:403740.

    Organism-specific databases

    CTDi 5730.

    Phylogenomic databases

    eggNOGi NOG45731.
    GeneTreei ENSGT00620000088005.
    HOGENOMi HOG000231660.
    HOVERGENi HBG106490.
    InParanoidi Q9XS65.
    KOi K01830.
    OrthoDBi EOG78PVBH.

    Enzyme and pathway databases

    Reactomei REACT_174506. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

    Miscellaneous databases

    NextBioi 20817242.

    Family and domain databases

    Gene3Di 2.40.128.20. 1 hit.
    InterProi IPR012674. Calycin.
    IPR011038. Calycin-like.
    IPR002345. Lipocalin.
    IPR000566. Lipocln_cytosolic_FA-bd_dom.
    IPR002972. PstgldnD_synth.
    [Graphical view ]
    Pfami PF00061. Lipocalin. 1 hit.
    [Graphical view ]
    PRINTSi PR00179. LIPOCALIN.
    PR01254. PGNDSYNTHASE.
    SUPFAMi SSF50814. SSF50814. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Dog lipocalin-type prostaglandin D synthase cDNA."
      Nakau H., Nakajima H., Urade Y., Fujimori K., Oda H., Seiki K.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-9.
      Tissue: Brain.

    Entry informationi

    Entry nameiPTGDS_CANFA
    AccessioniPrimary (citable) accession number: Q9XS65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3