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Q9XQ94

- GLNA2_MEDSA

UniProt

Q9XQ94 - GLNA2_MEDSA

Protein

Glutamine synthetase leaf isozyme, chloroplastic

Gene

GS2

Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.By similarity

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase leaf isozyme, chloroplastic (EC:6.3.1.2)
    Alternative name(s):
    GS2
    Glutamate--ammonia ligase
    Gene namesi
    Name:GS2
    Synonyms:GLN
    OrganismiMedicago sativa (Alfalfa)
    Taxonomic identifieri3879 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4949ChloroplastBy similarityAdd
    BLAST
    Chaini50 – 428379Glutamine synthetase leaf isozyme, chloroplasticPRO_0000011180Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XQ94.
    SMRiQ9XQ94. Positions 62-411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9XQ94-1 [UniParc]FASTAAdd to Basket

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    MAQILAPSIQ CQTRITKTSP LATPISSKMW SSLVMKQNKK VARSAKFRVM    50
    AINSGTINRV EDLLNLDITP FTDSIIAEYI WIGGTGIDVR SKSRTISKPV 100
    EHPSELPKWN YDGSSTGQAP GEDSEVILYP QAIFKDPFRG GNNILVICDA 150
    YTPQGEPIPT NKRHKAAEIF SNPKVEAEIP WYGIEQEYTL LQTDVKWPLG 200
    WPVGGYPGPQ GPYYCAAGAD KSFGRDISDA HYKACLYAGI NISGTNGEVM 250
    PGQWEYQVGP SVGIEAGDHI WASRYILERI TEQAGVVLTL DPKPIEGDWN 300
    GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHKVHIEAY GEGNERRLTG 350
    KHETASINTF SWGVANRGCS IRVGRDTEKN GKGYLEDRRP ASNMDPYVVT 400
    ALLAESTLLW EPTLEAEALA AQKIALKV 428
    Length:428
    Mass (Da):47,115
    Last modified:November 1, 1999 - v1
    Checksum:i2E37FB1957EA54C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF124244 mRNA. Translation: AAD28443.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF124244 mRNA. Translation: AAD28443.1 .

    3D structure databases

    ProteinModelPortali Q9XQ94.
    SMRi Q9XQ94. Positions 62-411.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Glutamine synthetase gene isolation from an alfalfa leaf cDNA library."
      Zozaya-Garza M., Sengupta-Gopalan C.
      Plant Gene Register PGR99-054
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Saranac.
      Tissue: Leaf.

    Entry informationi

    Entry nameiGLNA2_MEDSA
    AccessioniPrimary (citable) accession number: Q9XQ94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).By similarity

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3