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Protein

Glutamine synthetase leaf isozyme, chloroplastic

Gene

GS2

Organism
Medicago sativa (Alfalfa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase leaf isozyme, chloroplastic (EC:6.3.1.2)
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene namesi
Name:GS2
Synonyms:GLN
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949ChloroplastBy similarityAdd
BLAST
Chaini50 – 428379Glutamine synthetase leaf isozyme, chloroplasticPRO_0000011180Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9XQ94.
SMRiQ9XQ94. Positions 62-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XQ94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQILAPSIQ CQTRITKTSP LATPISSKMW SSLVMKQNKK VARSAKFRVM
60 70 80 90 100
AINSGTINRV EDLLNLDITP FTDSIIAEYI WIGGTGIDVR SKSRTISKPV
110 120 130 140 150
EHPSELPKWN YDGSSTGQAP GEDSEVILYP QAIFKDPFRG GNNILVICDA
160 170 180 190 200
YTPQGEPIPT NKRHKAAEIF SNPKVEAEIP WYGIEQEYTL LQTDVKWPLG
210 220 230 240 250
WPVGGYPGPQ GPYYCAAGAD KSFGRDISDA HYKACLYAGI NISGTNGEVM
260 270 280 290 300
PGQWEYQVGP SVGIEAGDHI WASRYILERI TEQAGVVLTL DPKPIEGDWN
310 320 330 340 350
GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHKVHIEAY GEGNERRLTG
360 370 380 390 400
KHETASINTF SWGVANRGCS IRVGRDTEKN GKGYLEDRRP ASNMDPYVVT
410 420
ALLAESTLLW EPTLEAEALA AQKIALKV
Length:428
Mass (Da):47,115
Last modified:November 1, 1999 - v1
Checksum:i2E37FB1957EA54C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124244 mRNA. Translation: AAD28443.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124244 mRNA. Translation: AAD28443.1.

3D structure databases

ProteinModelPortaliQ9XQ94.
SMRiQ9XQ94. Positions 62-411.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Glutamine synthetase gene isolation from an alfalfa leaf cDNA library."
    Zozaya-Garza M., Sengupta-Gopalan C.
    Plant Gene Register PGR99-054
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Saranac.
    Tissue: Leaf.

Entry informationi

Entry nameiGLNA2_MEDSA
AccessioniPrimary (citable) accession number: Q9XQ94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: November 1, 1999
Last modified: January 7, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).By similarity

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.