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Q9XQ94 (GLNA2_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase leaf isozyme, chloroplastic

EC=6.3.1.2
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene names
Name:GS2
Synonyms:GLN
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration By similarity.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Plastidchloroplast.

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT) By similarity.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast By similarity
Chain50 – 428379Glutamine synthetase leaf isozyme, chloroplastic
PRO_0000011180

Sequences

Sequence LengthMass (Da)Tools
Q9XQ94 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 2E37FB1957EA54C3

FASTA42847,115
        10         20         30         40         50         60 
MAQILAPSIQ CQTRITKTSP LATPISSKMW SSLVMKQNKK VARSAKFRVM AINSGTINRV 

        70         80         90        100        110        120 
EDLLNLDITP FTDSIIAEYI WIGGTGIDVR SKSRTISKPV EHPSELPKWN YDGSSTGQAP 

       130        140        150        160        170        180 
GEDSEVILYP QAIFKDPFRG GNNILVICDA YTPQGEPIPT NKRHKAAEIF SNPKVEAEIP 

       190        200        210        220        230        240 
WYGIEQEYTL LQTDVKWPLG WPVGGYPGPQ GPYYCAAGAD KSFGRDISDA HYKACLYAGI 

       250        260        270        280        290        300 
NISGTNGEVM PGQWEYQVGP SVGIEAGDHI WASRYILERI TEQAGVVLTL DPKPIEGDWN 

       310        320        330        340        350        360 
GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHKVHIEAY GEGNERRLTG KHETASINTF 

       370        380        390        400        410        420 
SWGVANRGCS IRVGRDTEKN GKGYLEDRRP ASNMDPYVVT ALLAESTLLW EPTLEAEALA 


AQKIALKV 

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References

[1]"Glutamine synthetase gene isolation from an alfalfa leaf cDNA library."
Zozaya-Garza M., Sengupta-Gopalan C.
Plant Gene Register PGR99-054
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Saranac.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF124244 mRNA. Translation: AAD28443.1.

3D structure databases

ProteinModelPortalQ9XQ94.
SMRQ9XQ94. Positions 62-411.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA2_MEDSA
AccessionPrimary (citable) accession number: Q9XQ94
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: November 1, 1999
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families