Reviewed, UniProtKB/Swiss-Prot Q9XJ38 (CAO_DUNSA)

Names and origin

Protein names

Recommended name:
    Chlorophyllide a oxygenase, chloroplastic
      Short name=Chlorophyll a oxygenase
    EC=1.13.12.14
Alternative name(s):
    Chlorophyll b synthase

Gene names

Name:

CAO

Organism

Dunaliella salina

Taxonomic identifier

3046 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Dunaliellaceae › Dunaliella

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes a two-step oxygenase reaction involved in the synthesis of chlorophyll b. Acts specifically on the non-esterified chlorophyllide a and not on chlorophyll a.
Catalytic activity	Chlorophyllide a + O(2) + NADPH = 7-hydroxychlorophyllide a + H(2)O + NADP(+). 7-hydroxychlorophyllide a + O(2) + NADPH = chlorophyllide b + 2 H(2)O + NADP(+).
Subcellular location	Plastid › chloroplast inner membrane; Peripheral membrane protein. Plastid › chloroplast thylakoid membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis
Cellular component	Chloroplast Membrane Plastid Plastid inner membrane
Domain	Coiled coil Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW plastid inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chlorophyllide a oxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 463		Chlorophyllide a oxygenase, chloroplastic		PRO_0000250165
Regions
	Domain	134 – 235	102	Rieske
	Coiled coil	1 – 51	51	Potential
Sites
	Metal binding	175	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	177	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	194	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	197	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	275	1	Iron By similarity
	Metal binding	279	1	Iron By similarity
	Metal binding	282	1	Iron By similarity
	Metal binding	287	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XJ38-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: FD37B01A3667DAAE	FASTA	463	51,532
	10 20 30 40 50 60 MQSKLLGLQD EISEARDKLR TSEARVAQNL KRVDELKAEA ASLERMRLAS SSSTDSTVSI 70 80 90 100 110 120 ASRGGAAVAA TTSVPDHVER EGIQSRVRGS GMASTSYPSH VPQPSQAVRR GPKPKDSRRL 130 140 150 160 170 180 RSSLELEDGL RNFWYPTEFA KKLEPGMMVP FDLFGVPWVL FRDEHSAPTC IKDSCAHRAC 190 200 210 220 230 240 PLSLGKVING HVQCPYHGWE FDGSGACTKM PSTRMCHGVG VAALPCVEKD GFVWVWPGDG 250 260 270 280 290 300 PPPDLPPDFT APPAGYDVHA EIMVDVPVEH GLLMENLLDL AHAPFTHTTT FARGWPIPEA 310 320 330 340 350 360 VRFHATKMLA GDWDPYPISM SFNPPCIALS TIGLSQPGKI MRGYKAEECK RHLHQLHVCM 370 380 390 400 410 420 PSKEGHTRLL YRMSLDFWGW AKHVPFVDVL WKKIAGQVLG EDLVLVLGQQ ARMIGGDDTW 430 440 450 460 CTPMPYDKLA VRYRRWRNMV ADGEYEEGSR NRCTSQYDSW PDV

References

[1]	"Chlorophyll b and phycobilins in the common ancestor of cyanobacteria and chloroplasts." Tomitani A., Okada K., Miyashita H., Matthijs H.C.P., Ohno T., Tanaka A. Nature 400:159-162(1999) [PubMed: 10408441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB021312 mRNA. Translation: BAA82481.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013626. PaO. IPR005806. Rieske_reg. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
Pfam	PF08417. PaO. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

CAO_DUNSA

Accession

Primary (citable) accession number: Q9XJ38

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	November 1, 1999
Last modified:	November 4, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)