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Q9XIW1 (XTH5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 5

Short name=At-XTH5
Short name=XTH-5
EC=2.4.1.207
Gene names
Name:XTH5
Synonyms:EXGT-A4, XTR12
Ordered Locus Names:At5g13870
ORF Names:MAC12.33
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Root specific. Ref.1 Ref.5

Induction

By brassinolide. Strongly down-regulated by abscisic acid. Ref.5

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 293272Probable xyloglucan endotransglucosylase/hydrolase protein 5
PRO_0000011805

Sites

Active site1061Nucleophile By similarity
Active site1101Proton donor By similarity

Amino acid modifications

Glycosylation1141N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9XIW1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 1025767DDA2BA9F4

FASTA29333,949
        10         20         30         40         50         60 
MGRLSSTLCL TFLILATVAF GVPPKKSINV PFGRNYFPTW AFDHIKYLNG GSEVHLVLDK 

        70         80         90        100        110        120 
YTGTGFQSKG SYLFGHFSMH IKMVAGDSAG TVTAFYLSSQ NSEHDEIDFE FLGNRTGQPY 

       130        140        150        160        170        180 
ILQTNVFTGG AGNREQRINL WFDPSKDYHS YSVLWNMYQI VFFVDDVPIR VFKNSKDVGV 

       190        200        210        220        230        240 
KFPFNQPMKI YSSLWNADDW ATRGGLEKTN WEKAPFVASY RGFHVDGCEA SVNAKFCETQ 

       250        260        270        280        290 
GKRWWDQKEF QDLDANQYKR LKWVRKRYTI YNYCTDRVRF PVPPPECRRD RDI 

« Hide

References

« Hide 'large scale' references
[1]"Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Endoxyloglucan transferase homologue from Arabidopsis thaliana."
Okamoto S., Hayashida N., Shinozaki K., Nishitani K.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
Yokoyama R., Nishitani K.
Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[6]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF163822 Genomic DNA. Translation: AAD45126.1.
AB026486 mRNA. Translation: BAA81669.1.
AB005230 Genomic DNA. Translation: BAB11115.1.
CP002688 Genomic DNA. Translation: AED91952.1.
RefSeqNP_196891.1. NM_121390.4.
UniGeneAt.364.

3D structure databases

ProteinModelPortalQ9XIW1.
SMRQ9XIW1. Positions 30-293.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbQ9XIW1.
PRIDEQ9XIW1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G13870.1; AT5G13870.1; AT5G13870.
GeneID831233.
KEGGath:AT5G13870.

Organism-specific databases

GeneFarm2636. 265.
TAIRAT5G13870.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidQ9XIW1.
KOK08235.
OMAPPECRRD.
PhylomeDBQ9XIW1.
ProtClustDBCLSN2683385.

Enzyme and pathway databases

BioCycARA:AT5G13870-MONOMER.

Gene expression databases

GenevestigatorQ9XIW1.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH5_ARATH
AccessionPrimary (citable) accession number: Q9XIW1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names