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Q9XIW1

- XTH5_ARATH

UniProt

Q9XIW1 - XTH5_ARATH

Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 5

Gene

XTH5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.By similarity

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei106 – 1061NucleophilePROSITE-ProRule annotation
    Active sitei110 – 1101Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT5G13870-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable xyloglucan endotransglucosylase/hydrolase protein 5 (EC:2.4.1.207)
    Short name:
    At-XTH5
    Short name:
    XTH-5
    Gene namesi
    Name:XTH5
    Synonyms:EXGT-A4, XTR12
    Ordered Locus Names:At5g13870
    ORF Names:MAC12.33
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G13870.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 293272Probable xyloglucan endotransglucosylase/hydrolase protein 5PRO_0000011805Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9XIW1.
    PRIDEiQ9XIW1.

    Expressioni

    Tissue specificityi

    Root specific.2 Publications

    Inductioni

    By brassinolide. Strongly down-regulated by abscisic acid.1 Publication

    Gene expression databases

    GenevestigatoriQ9XIW1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XIW1.
    SMRiQ9XIW1. Positions 30-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000236368.
    InParanoidiQ9XIW1.
    KOiK08235.
    OMAiNAKFCET.
    PhylomeDBiQ9XIW1.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9XIW1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRLSSTLCL TFLILATVAF GVPPKKSINV PFGRNYFPTW AFDHIKYLNG    50
    GSEVHLVLDK YTGTGFQSKG SYLFGHFSMH IKMVAGDSAG TVTAFYLSSQ 100
    NSEHDEIDFE FLGNRTGQPY ILQTNVFTGG AGNREQRINL WFDPSKDYHS 150
    YSVLWNMYQI VFFVDDVPIR VFKNSKDVGV KFPFNQPMKI YSSLWNADDW 200
    ATRGGLEKTN WEKAPFVASY RGFHVDGCEA SVNAKFCETQ GKRWWDQKEF 250
    QDLDANQYKR LKWVRKRYTI YNYCTDRVRF PVPPPECRRD RDI 293
    Length:293
    Mass (Da):33,949
    Last modified:November 1, 1999 - v1
    Checksum:i1025767DDA2BA9F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF163822 Genomic DNA. Translation: AAD45126.1.
    AB026486 mRNA. Translation: BAA81669.1.
    AB005230 Genomic DNA. Translation: BAB11115.1.
    CP002688 Genomic DNA. Translation: AED91952.1.
    RefSeqiNP_196891.1. NM_121390.4.
    UniGeneiAt.364.

    Genome annotation databases

    EnsemblPlantsiAT5G13870.1; AT5G13870.1; AT5G13870.
    GeneIDi831233.
    KEGGiath:AT5G13870.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF163822 Genomic DNA. Translation: AAD45126.1 .
    AB026486 mRNA. Translation: BAA81669.1 .
    AB005230 Genomic DNA. Translation: BAB11115.1 .
    CP002688 Genomic DNA. Translation: AED91952.1 .
    RefSeqi NP_196891.1. NM_121390.4.
    UniGenei At.364.

    3D structure databases

    ProteinModelPortali Q9XIW1.
    SMRi Q9XIW1. Positions 30-293.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PaxDbi Q9XIW1.
    PRIDEi Q9XIW1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G13870.1 ; AT5G13870.1 ; AT5G13870 .
    GeneIDi 831233.
    KEGGi ath:AT5G13870.

    Organism-specific databases

    GeneFarmi 2636. 265.
    TAIRi AT5G13870.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000236368.
    InParanoidi Q9XIW1.
    KOi K08235.
    OMAi NAKFCET.
    PhylomeDBi Q9XIW1.

    Enzyme and pathway databases

    BioCyci ARA:AT5G13870-MONOMER.

    Gene expression databases

    Genevestigatori Q9XIW1.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
      Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
      Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Endoxyloglucan transferase homologue from Arabidopsis thaliana."
      Okamoto S., Hayashida N., Shinozaki K., Nishitani K.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
      Yokoyama R., Nishitani K.
      Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    6. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiXTH5_ARATH
    AccessioniPrimary (citable) accession number: Q9XIW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3