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Protein

CBL-interacting serine/threonine-protein kinase 7

Gene

CIPK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity). Phosphorylates the rice sucrose synthase (SuSy) in vitro in an allosteric manner. Involved in cold response.By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei149Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • protein phosphorylation Source: GO_Central
  • response to cold Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 7 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.10
SOS2-like protein kinase PKS7
Serine/threonine-protein kinase SR2
Short name:
AtSR2
Short name:
AtSRPK1
Gene namesi
Name:CIPK7
Synonyms:PKS7, SnRK3.10, SR2, SRPK1
Ordered Locus Names:At3g23000
ORF Names:MXC7.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G23000
TAIRilocus:2094553 AT3G23000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003372101 – 429CBL-interacting serine/threonine-protein kinase 7Add BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171PhosphoserineBy similarity1
Modified residuei184PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9XIW0

Expressioni

Tissue specificityi

Strongly expressed in leaves, but barely expressed in roots, stems or flowers.2 Publications

Inductioni

By sucrose, glucose and fructose. Repressed in roots by salt stress. Up-regulated by cold stress.3 Publications

Gene expression databases

ExpressionAtlasiQ9XIW0 baseline and differential
GenevisibleiQ9XIW0 AT

Interactioni

Subunit structurei

Interacts with CBL1, CBL2 and CBL3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CBL1O814453EBI-1765255,EBI-974530

Protein-protein interaction databases

BioGridi7206, 7 interactors
IntActiQ9XIW0, 6 interactors
STRINGi3702.AT3G23000.1

Structurei

3D structure databases

ProteinModelPortaliQ9XIW0
SMRiQ9XIW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 280Protein kinasePROSITE-ProRule annotationAdd BLAST256
Domaini302 – 326NAFPROSITE-ProRule annotationAdd BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 195Activation loopBy similarityAdd BLAST29
Regioni330 – 363PPIBy similarityAdd BLAST34

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
HOGENOMiHOG000233016
InParanoidiQ9XIW0
OMAiANIADMC
OrthoDBiEOG09360G05
PhylomeDBiQ9XIW0

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR018451 NAF/FISL_domain
IPR004041 NAF_dom
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF03822 NAF, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50816 NAF, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9XIW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLPQPQNQ SSPATTPAKI LLGKYELGRR LGSGSFAKVH LARSIESDEL
60 70 80 90 100
VAVKIIEKKK TIESGMEPRI IREIDAMRRL RHHPNILKIH EVMATKSKIY
110 120 130 140 150
LVMELASGGE LFSKVLRRGR LPESTARRYF QQLASALRFS HQDGVAHRDV
160 170 180 190 200
KPQNLLLDEQ GNLKVSDFGL SALPEHLQNG LLHTACGTPA YTAPEVISRR
210 220 230 240 250
GYDGAKADAW SCGVILFVLL VGDVPFDDSN IAAMYRKIHR RDYRFPSWIS
260 270 280 290 300
KQAKSIIYQM LDPNPVTRMS IETVMKTNWF KKSLETSEFH RNVFDSEVEM
310 320 330 340 350
KSSVNSITAF DLISLSSGLD LSGLFEAKKK KERRFTAKVS GVEVEEKAKM
360 370 380 390 400
IGEKLGYVVK KKMMKKEGEV KVVGLGRGRT VIVVEAVELT VDVVVVEVKV
410 420
VEGEEDDSRW SDLITELEDI VLSWHNDIM
Length:429
Mass (Da):48,266
Last modified:November 1, 1999 - v1
Checksum:i5D6703058ACF4A75
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25Y → H in BAB11738 (PubMed:11212922).Curated1
Sequence conflicti224V → A in AAK96877 (PubMed:14593172).Curated1
Sequence conflicti336T → P in AAK96877 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290192 mRNA Translation: AAK16682.1
AB035148 mRNA Translation: BAB11738.1
AB027153 mRNA Translation: BAA77716.2
AF339148 mRNA Translation: AAK26846.1
AB026655 Genomic DNA Translation: BAB02091.1
CP002686 Genomic DNA Translation: AEE76704.1
AY054686 mRNA Translation: AAK96877.1
BT020492 mRNA Translation: AAW38993.1
BT028967 mRNA Translation: ABI54342.1
RefSeqiNP_188940.1, NM_113200.3
UniGeneiAt.389
At.71615

Genome annotation databases

EnsemblPlantsiAT3G23000.1; AT3G23000.1; AT3G23000
GeneIDi821874
GrameneiAT3G23000.1; AT3G23000.1; AT3G23000
KEGGiath:AT3G23000

Similar proteinsi

Entry informationi

Entry nameiCIPK7_ARATH
AccessioniPrimary (citable) accession number: Q9XIW0
Secondary accession number(s): Q93Y18, Q9FZN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 1, 1999
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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