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Protein

U-box domain-containing protein 18

Gene

PUB18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an E3 ubiquitin ligase (By similarity). Mediates EXO70B1 ubiquitination. Involved in the regulation of abscisic acid (ABA)-mediated stomatal movements (PubMed:27956469).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: UniProtKB
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • protein ubiquitination Source: TAIR
  • regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  • regulation of seed germination Source: TAIR
  • regulation of stomatal movement Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processAbscisic acid signaling pathway, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
U-box domain-containing protein 181 Publication (EC:2.3.2.271 Publication)
Alternative name(s):
Plant U-box protein 181 Publication
RING-type E3 ubiquitin transferase PUB181 Publication
Gene namesi
Name:PUB181 Publication
Ordered Locus Names:At1g10560Imported
ORF Names:T10O24.19Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G10560.
TAIRilocus:2194564. AT1G10560.

Subcellular locationi

  • Endomembrane system 1 Publication

  • Note: Displays a punctate pattern. Colocalizes with EXO70B1.1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23 – 210Missing : Impaired EXO70B1 binding. Altered ubiquitination of EXO70B1, but unusual binding and ubiquitination of EXO70B2. Reduced abscisic acid (ABA)-mediated stomatal movements. 1 PublicationAdd BLAST188
Mutagenesisi305V → I: Abrogated E3 ligase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003221621 – 697U-box domain-containing protein 18Add BLAST697

Proteomic databases

PaxDbiQ9XIJ5.
PRIDEiQ9XIJ5.

PTM databases

iPTMnetiQ9XIJ5.

Expressioni

Gene expression databases

GenevisibleiQ9XIJ5. AT.

Interactioni

Subunit structurei

Interacts with EXO70B1 via its U-box N-terminal domain (UND).1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G10560.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XIJ5.
SMRiQ9XIJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini287 – 361U-boxPROSITE-ProRule annotationAdd BLAST75
Repeati420 – 459ARM 1Sequence analysisAdd BLAST40
Repeati461 – 500ARM 2Sequence analysisAdd BLAST40
Repeati502 – 544ARM 3Sequence analysisAdd BLAST43
Repeati546 – 587ARM 4Sequence analysisAdd BLAST42
Repeati589 – 631ARM 5Sequence analysisAdd BLAST43
Repeati657 – 696ARM 6Sequence analysisAdd BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 210U-box N-terminal domain (UND) required for EXO70B1 binding and crucial for the negative regulation of ABA-dependent stomatal movement1 PublicationAdd BLAST188

Domaini

The U-box N-terminal domain (UND) confers binding specificity and subsequent ubiquitination.1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITM9. Eukaryota.
ENOG410YCUS. LUCA.
HOGENOMiHOG000238412.
InParanoidiQ9XIJ5.
OMAiKWFRAGN.
OrthoDBiEOG093604QN.
PhylomeDBiQ9XIJ5.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF00514. Arm. 1 hit.
PF04564. U-box. 1 hit.
SMARTiView protein in SMART
SM00185. ARM. 4 hits.
SM00504. Ubox. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiView protein in PROSITE
PS50176. ARM_REPEAT. 1 hit.
PS51698. U_BOX. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9XIJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHTKTGSGR RILTFPTVEP SESISIVTLL DSLIQLAGDI LTFKSKHFST
60 70 80 90 100
NKQSFRETLR RIQNLLVVFE EIRIRIRNSR RYFHDSAAAS SLKEIHVGFQ
110 120 130 140 150
KLKFLLEDCT RDGARLCMMM NSDQVSDHLR VLTRSISTSL SAFPVASVDL
160 170 180 190 200
TTEVNELIDL VVRQARKYGV QPETNDKRAV SSINRILALF VNRVVPDPDE
210 220 230 240 250
INRILDHVGI RKWGDCVKEI NFLGEEIDAE RLDEKKKKSS DQVELLSSLM
260 270 280 290 300
GFICYCRCII LGRIERDDHH NHHEDGIKKD HDLIRGLKVE DLLCPISLEI
310 320 330 340 350
MTDPVVIETG HTYDRSSITK WFGSGNITCP ITGKILTSTE LVDNVSVRQV
360 370 380 390 400
IRKHCKTNGI VLAGISRRRK SHDDVVPESL AAKGAGKLIA KFLTSELING
410 420 430 440 450
GEEMIYRAVR EIRVQTKTSS FNRSCLVKAG AVTPLLKLLS SVDIRIQENA
460 470 480 490 500
MAGILNLSKH VTGKSKIAGE GLKILVEILN EGAKTETRLY SASALFYLSS
510 520 530 540 550
VEDYSRLIGE NPDAIPGLMN IVKGDDYGDS AKRSALLAVM GLLMQSDNHW
560 570 580 590 600
RVLAAGAVPI LLDLLRSGEI SGGLTADCLA TLAKLAEYPD GTIGVIRRGG
610 620 630 640 650
LKLAVKILSS SEDSPVAVKQ HCVGLILNLC LNGGRDVVGV LVKNSLVMGS
660 670 680 690
LYTVLSNGEY GGSKKASALI RMIHEFQERK TGSVEPNLQR GRFVHAW
Length:697
Mass (Da):76,995
Last modified:November 1, 1999 - v1
Checksum:iF014FE42FC256F09
GO

Sequence cautioni

The sequence AAL77690 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti648M → V in AAL77690 (PubMed:14593172).Curated1
Sequence conflicti688L → H in AAL77690 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007067 Genomic DNA. Translation: AAD39579.1.
CP002684 Genomic DNA. Translation: AEE28594.1.
AY075683 mRNA. Translation: AAL77690.1. Different initiation.
PIRiB86239.
RefSeqiNP_172526.1. NM_100931.3.
UniGeneiAt.14982.

Genome annotation databases

EnsemblPlantsiAT1G10560.1; AT1G10560.1; AT1G10560.
GeneIDi837597.
GrameneiAT1G10560.1; AT1G10560.1; AT1G10560.
KEGGiath:AT1G10560.

Similar proteinsi

Entry informationi

Entry nameiPUB18_ARATH
AccessioniPrimary (citable) accession number: Q9XIJ5
Secondary accession number(s): Q8S9I5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 1, 1999
Last modified: August 30, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways