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Protein

U-box domain-containing protein 18

Gene

PUB18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as an E3 ubiquitin ligase.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • protein ubiquitination Source: TAIR
  • regulation of seed germination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
U-box domain-containing protein 18 (EC:6.3.2.-)
Alternative name(s):
Plant U-box protein 18
Gene namesi
Name:PUB18
Ordered Locus Names:At1g10560
ORF Names:T10O24.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G10560.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 697697U-box domain-containing protein 18PRO_0000322162Add
BLAST

Proteomic databases

PaxDbiQ9XIJ5.
PRIDEiQ9XIJ5.

PTM databases

iPTMnetiQ9XIJ5.

Expressioni

Gene expression databases

GenevisibleiQ9XIJ5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G10560.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XIJ5.
SMRiQ9XIJ5. Positions 290-360, 431-457, 623-654.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini287 – 36175U-boxAdd
BLAST
Repeati420 – 45940ARM 1Add
BLAST
Repeati461 – 50040ARM 2Add
BLAST
Repeati502 – 54443ARM 3Add
BLAST
Repeati546 – 58742ARM 4Add
BLAST
Repeati589 – 63143ARM 5Add
BLAST

Sequence similaritiesi

Contains 5 ARM repeats.PROSITE-ProRule annotation
Contains 1 U-box domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITM9. Eukaryota.
ENOG410YCUS. LUCA.
HOGENOMiHOG000238412.
InParanoidiQ9XIJ5.
OMAiVIETGHT.
OrthoDBiEOG093604QN.
PhylomeDBiQ9XIJ5.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00514. Arm. 1 hit.
PF04564. U-box. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 4 hits.
SM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
PS51698. U_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XIJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHTKTGSGR RILTFPTVEP SESISIVTLL DSLIQLAGDI LTFKSKHFST
60 70 80 90 100
NKQSFRETLR RIQNLLVVFE EIRIRIRNSR RYFHDSAAAS SLKEIHVGFQ
110 120 130 140 150
KLKFLLEDCT RDGARLCMMM NSDQVSDHLR VLTRSISTSL SAFPVASVDL
160 170 180 190 200
TTEVNELIDL VVRQARKYGV QPETNDKRAV SSINRILALF VNRVVPDPDE
210 220 230 240 250
INRILDHVGI RKWGDCVKEI NFLGEEIDAE RLDEKKKKSS DQVELLSSLM
260 270 280 290 300
GFICYCRCII LGRIERDDHH NHHEDGIKKD HDLIRGLKVE DLLCPISLEI
310 320 330 340 350
MTDPVVIETG HTYDRSSITK WFGSGNITCP ITGKILTSTE LVDNVSVRQV
360 370 380 390 400
IRKHCKTNGI VLAGISRRRK SHDDVVPESL AAKGAGKLIA KFLTSELING
410 420 430 440 450
GEEMIYRAVR EIRVQTKTSS FNRSCLVKAG AVTPLLKLLS SVDIRIQENA
460 470 480 490 500
MAGILNLSKH VTGKSKIAGE GLKILVEILN EGAKTETRLY SASALFYLSS
510 520 530 540 550
VEDYSRLIGE NPDAIPGLMN IVKGDDYGDS AKRSALLAVM GLLMQSDNHW
560 570 580 590 600
RVLAAGAVPI LLDLLRSGEI SGGLTADCLA TLAKLAEYPD GTIGVIRRGG
610 620 630 640 650
LKLAVKILSS SEDSPVAVKQ HCVGLILNLC LNGGRDVVGV LVKNSLVMGS
660 670 680 690
LYTVLSNGEY GGSKKASALI RMIHEFQERK TGSVEPNLQR GRFVHAW
Length:697
Mass (Da):76,995
Last modified:November 1, 1999 - v1
Checksum:iF014FE42FC256F09
GO

Sequence cautioni

The sequence AAL77690 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti648 – 6481M → V in AAL77690 (PubMed:14593172).Curated
Sequence conflicti688 – 6881L → H in AAL77690 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007067 Genomic DNA. Translation: AAD39579.1.
CP002684 Genomic DNA. Translation: AEE28594.1.
AY075683 mRNA. Translation: AAL77690.1. Different initiation.
PIRiB86239.
RefSeqiNP_172526.1. NM_100931.2.
UniGeneiAt.14982.

Genome annotation databases

EnsemblPlantsiAT1G10560.1; AT1G10560.1; AT1G10560.
GeneIDi837597.
GrameneiAT1G10560.1; AT1G10560.1; AT1G10560.
KEGGiath:AT1G10560.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007067 Genomic DNA. Translation: AAD39579.1.
CP002684 Genomic DNA. Translation: AEE28594.1.
AY075683 mRNA. Translation: AAL77690.1. Different initiation.
PIRiB86239.
RefSeqiNP_172526.1. NM_100931.2.
UniGeneiAt.14982.

3D structure databases

ProteinModelPortaliQ9XIJ5.
SMRiQ9XIJ5. Positions 290-360, 431-457, 623-654.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G10560.1.

PTM databases

iPTMnetiQ9XIJ5.

Proteomic databases

PaxDbiQ9XIJ5.
PRIDEiQ9XIJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G10560.1; AT1G10560.1; AT1G10560.
GeneIDi837597.
GrameneiAT1G10560.1; AT1G10560.1; AT1G10560.
KEGGiath:AT1G10560.

Organism-specific databases

TAIRiAT1G10560.

Phylogenomic databases

eggNOGiENOG410ITM9. Eukaryota.
ENOG410YCUS. LUCA.
HOGENOMiHOG000238412.
InParanoidiQ9XIJ5.
OMAiVIETGHT.
OrthoDBiEOG093604QN.
PhylomeDBiQ9XIJ5.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9XIJ5.

Gene expression databases

GenevisibleiQ9XIJ5. AT.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00514. Arm. 1 hit.
PF04564. U-box. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 4 hits.
SM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
PS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUB18_ARATH
AccessioniPrimary (citable) accession number: Q9XIJ5
Secondary accession number(s): Q8S9I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.