UniProtKB - Q9XIE6 (ALA3_ARATH)
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Protein
Phospholipid-transporting ATPase 3
Gene
ALA3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Required for secretory processes during plant development. Requires an interaction with an ALIS protein for activity. Has activity with phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine, but not with lysolipid (PubMed:18344284, PubMed:19566596, PubMed:20053675). Modifies endomembranes in multiple cell types, enabling structural changes, or signaling functions that are critical for normal development and adaptation to varied growth environments (PubMed:23667493).4 Publications
Miscellaneous
The intracellular targeting signals and lipid specificity determinants reside in the catalytic ALA subunit.1 Publication
Catalytic activityi
ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 413 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 845 | MagnesiumBy similarity | 1 | |
Metal bindingi | 849 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: InterPro
- phospholipid-translocating ATPase activity Source: GO_Central
- phospholipid transporter activity Source: TAIR
GO - Biological processi
- Golgi vesicle budding Source: TAIR
- root development Source: TAIR
- shoot system development Source: TAIR
Keywordsi
Molecular function | Hydrolase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G59820-MONOMER. |
BRENDAi | 3.6.99.B1. 399. |
Reactomei | R-ATH-6798695. Neutrophil degranulation. R-ATH-936837. Ion transport by P-type ATPases. |
Protein family/group databases
TCDBi | 3.A.3.8.6. the p-type atpase (p-atpase) superfamily. |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipid-transporting ATPase 31 Publication (EC:3.6.3.11 Publication)Short name: AtALA31 Publication Alternative name(s): Aminophospholipid ATPase 31 Publication Aminophospholipid flippase 31 Publication Protein IRREGULAR TRICHOME BRANCH 21 Publication |
Gene namesi | Name:ALA31 Publication Synonyms:ITB21 Publication Ordered Locus Names:At1g59820Imported ORF Names:F23H11.14Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G59820. |
TAIRi | locus:2025961. AT1G59820. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 67 | CytoplasmicSequence analysisAdd BLAST | 67 | |
Transmembranei | 68 – 89 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 90 – 93 | ExtracellularSequence analysis | 4 | |
Transmembranei | 94 – 116 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 117 – 297 | CytoplasmicSequence analysisAdd BLAST | 181 | |
Transmembranei | 298 – 319 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 320 – 346 | ExtracellularSequence analysisAdd BLAST | 27 | |
Transmembranei | 347 – 364 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 365 – 900 | CytoplasmicSequence analysisAdd BLAST | 536 | |
Transmembranei | 901 – 920 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 921 – 934 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 935 – 954 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 955 – 984 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 985 – 1006 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 1007 – 1013 | ExtracellularSequence analysis | 7 | |
Transmembranei | 1014 – 1036 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1037 – 1042 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 1043 – 1063 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1064 – 1076 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 1077 – 1087 | HelicalSequence analysisAdd BLAST | 11 | |
Topological domaini | 1088 – 1213 | CytoplasmicSequence analysisAdd BLAST | 126 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Impaired growth of roots and shoots (PubMed:18344284, PubMed:23667493). Roots devoided of the characteristic trans-Golgi proliferation of slime vesicles containing polysaccharides and enzymes for secretion (PubMed:18344284). Aberrant trichome expansion, reduced primary root growth and longer root hairs (PubMed:19566596). Impaired pollen growth (PubMed:19566596, PubMed:23667493). Impaired ovule development (PubMed:23667493). Reduced adaptability to temperature stresses (PubMed:23667493).3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 413 | D → A: Loss of internalization of phospholipids. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046387 | 1 – 1213 | Phospholipid-transporting ATPase 3Add BLAST | 1213 |
Proteomic databases
PaxDbi | Q9XIE6. |
PRIDEi | Q9XIE6. |
PTM databases
iPTMneti | Q9XIE6. |
Expressioni
Tissue specificityi
Expressed in petals and sepals, but not in reproductive tissues. In siliques, detected in the upper part of the seed pod and in the area between the seed pod and the stem, but not in developing seeds. Strong expression in vascular shoot tissues and in stomatal guard cells of young rosettes leaves. In roots, expressed in cells surrounding the xylem and in central and peripheral columella cells. Detected in developing and mature trichomes, roots, pollen and growing pollen tubes.2 Publications
Gene expression databases
ExpressionAtlasi | Q9XIE6. baseline and differential. |
Genevisiblei | Q9XIE6. AT. |
Interactioni
Subunit structurei
Associates with ALIS1 to form a stable and active complex. Interacts with ALIS3 and ALIS5 in a heterologous system.2 Publications
Protein-protein interaction databases
BioGridi | 27500. 1 interactor. |
STRINGi | 3702.AT1G59820.1. |
Family & Domainsi
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410ITKD. Eukaryota. ENOG410XPYK. LUCA. |
HOGENOMi | HOG000202528. |
InParanoidi | Q9XIE6. |
KOi | K14802. |
OMAi | RMRKQRG. |
OrthoDBi | EOG093600FE. |
PhylomeDBi | Q9XIE6. |
Family and domain databases
Gene3Di | 3.40.1110.10. 1 hit. 3.40.50.1000. 1 hit. |
InterProi | View protein in InterPro IPR023299. ATPase_P-typ_cyto_dom_N. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom_sf. IPR008250. ATPase_P-typ_transduc_dom_A_sf. IPR036412. HAD-like_sf. IPR023214. HAD_sf. IPR006539. P-type_ATPase_IV. IPR032631. P-type_ATPase_N. IPR001757. P_typ_ATPase. IPR032630. P_typ_ATPase_c. |
PANTHERi | PTHR24092. PTHR24092. 1 hit. |
Pfami | View protein in Pfam PF16212. PhoLip_ATPase_C. 1 hit. PF16209. PhoLip_ATPase_N. 1 hit. |
SUPFAMi | SSF56784. SSF56784. 3 hits. SSF81653. SSF81653. 2 hits. SSF81660. SSF81660. 2 hits. SSF81665. SSF81665. 3 hits. |
TIGRFAMsi | TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 1 hit. |
PROSITEi | View protein in PROSITE PS00154. ATPASE_E1_E2. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q9XIE6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVRSGSFSVD SSATHQRTPS RTVTLGHIQP QAPTYRTVYC NDRESNQPVR
60 70 80 90 100
FKGNSISTTK YNVFTFLPKG LFEQFRRIAN IYFLGISCLS MTPISPVSPI
110 120 130 140 150
TNVAPLSMVL LVSLIKEAFE DWKRFQNDMS INNSTVEILQ DQQWVSIPWR
160 170 180 190 200
KLQVGDIVKI KKDGFFPADI LFMSSTNSDG ICYVETANLD GETNLKIRKA
210 220 230 240 250
LERTWDYLVP EKAYEFKGEI QCEQPNNSLY TFTGNLVVQK QTLPLSPDQL
260 270 280 290 300
LLRGCSLRNT EYIVGAVVFT GHETKVMMNA MNAPSKRSTL EKKLDKLIIT
310 320 330 340 350
IFCVLVTMCL IGAIGCSIVT DREDKYLGLH NSDWEYRNGL MIGFFTFFTL
360 370 380 390 400
VTLFSSIIPI SLYVSIEMIK FIQSTQFINR DLNMYHAETN TPASARTSNL
410 420 430 440 450
NEELGQVEYI FSDKTGTLTR NLMEFFKCSI GGVSYGCGVT EIEKGIAQRH
460 470 480 490 500
GLKVQEEQRS TGAIREKGFN FDDPRLMRGA WRNEPNPDLC KELFRCLAIC
510 520 530 540 550
HTVLPEGDES PEKIVYQAAS PDEAALVTAA KNFGFFFYRR TPTMVYVRES
560 570 580 590 600
HVEKMGKIQD VAYEILNVLE FNSTRKRQSV VCRFPDGRLV LYCKGADNVI
610 620 630 640 650
FERLANGMDD VRKVTREHLE HFGSSGLRTL CLAYKDLNPE TYDSWNEKFI
660 670 680 690 700
QAKSALRDRE KKLDEVAELI EKDLILIGST AIEDKLQEGV PTCIETLSRA
710 720 730 740 750
GIKIWVLTGD KMETAINIAY ACNLINNEMK QFVISSETDA IREAEERGDQ
760 770 780 790 800
VEIARVIKEE VKRELKKSLE EAQHSLHTVA GPKLSLVIDG KCLMYALDPS
810 820 830 840 850
LRVMLLSLSL NCTSVVCCRV SPLQKAQVTS LVRKGAQKIT LSIGDGANDV
860 870 880 890 900
SMIQAAHVGI GISGMEGMQA VMASDFAIAQ FRFLTDLLLV HGRWSYLRIC
910 920 930 940 950
KVVMYFFYKN LTFTLTQFWF TFRTGFSGQR FYDDWFQSLF NVVFTALPVI
960 970 980 990 1000
VLGLFEKDVS ASLSKRYPEL YREGIRNSFF KWRVVAVWAT SAVYQSLVCY
1010 1020 1030 1040 1050
LFVTTSSFGA VNSSGKVFGL WDVSTMVFTC LVIAVNVRIL LMSNSITRWH
1060 1070 1080 1090 1100
YITVGGSILA WLVFAFVYCG IMTPHDRNEN VYFVIYVLMS TFYFYFTLLL
1110 1120 1130 1140 1150
VPIVSLLGDF IFQGVERWFF PYDYQIVQEI HRHESDASKA DQLEVENELT
1160 1170 1180 1190 1200
PQEARSYAIS QLPRELSKHT GFAFDSPGYE SFFASQLGIY APQKAWDVAR
1210
RASMRSRPKV PKK
Sequence cautioni
The sequence AAD39325 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007258 Genomic DNA. Translation: AAD39325.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE33621.1. AY091777 mRNA. Translation: AAM10325.1. |
PIRi | C96622. |
RefSeqi | NP_176191.1. NM_104675.3. |
UniGenei | At.36777. |
Genome annotation databases
EnsemblPlantsi | AT1G59820.1; AT1G59820.1; AT1G59820. |
GeneIDi | 842275. |
Gramenei | AT1G59820.1; AT1G59820.1; AT1G59820. |
KEGGi | ath:AT1G59820. |
Similar proteinsi
Entry informationi
Entry namei | ALA3_ARATH | |
Accessioni | Q9XIE6Primary (citable) accession number: Q9XIE6 Secondary accession number(s): Q8RWQ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | February 28, 2003 | |
Last modified: | March 28, 2018 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |