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UniProtKB - Q9XIE6 (ALA3_ARATH)

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Protein

Phospholipid-transporting ATPase 3

Gene

ALA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Required for secretory processes during plant development. Requires an interaction with an ALIS protein for activity. Has activity with phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine, but not with lysolipid (PubMed:18344284, PubMed:19566596, PubMed:20053675). Modifies endomembranes in multiple cell types, enabling structural changes, or signaling functions that are critical for normal development and adaptation to varied growth environments (PubMed:23667493).4 Publications

Miscellaneous

The intracellular targeting signals and lipid specificity determinants reside in the catalytic ALA subunit.1 Publication

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4134-aspartylphosphate intermediateBy similarity1
Metal bindingi845MagnesiumBy similarity1
Metal bindingi849MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

  • Golgi vesicle budding Source: TAIR
  • root development Source: TAIR
  • shoot system development Source: TAIR
Complete GO annotation...

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G59820-MONOMER.
BRENDAi3.6.99.B1. 399.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.8.6. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase 31 Publication (EC:3.6.3.11 Publication)
Short name:
AtALA31 Publication
Alternative name(s):
Aminophospholipid ATPase 31 Publication
Aminophospholipid flippase 31 Publication
Protein IRREGULAR TRICHOME BRANCH 21 Publication
Gene namesi
Name:ALA31 Publication
Synonyms:ITB21 Publication
Ordered Locus Names:At1g59820Imported
ORF Names:F23H11.14Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2025961. AT1G59820.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 67CytoplasmicSequence analysisAdd BLAST67
Transmembranei68 – 89HelicalSequence analysisAdd BLAST22
Topological domaini90 – 93ExtracellularSequence analysis4
Transmembranei94 – 116HelicalSequence analysisAdd BLAST23
Topological domaini117 – 297CytoplasmicSequence analysisAdd BLAST181
Transmembranei298 – 319HelicalSequence analysisAdd BLAST22
Topological domaini320 – 346ExtracellularSequence analysisAdd BLAST27
Transmembranei347 – 364HelicalSequence analysisAdd BLAST18
Topological domaini365 – 900CytoplasmicSequence analysisAdd BLAST536
Transmembranei901 – 920HelicalSequence analysisAdd BLAST20
Topological domaini921 – 934ExtracellularSequence analysisAdd BLAST14
Transmembranei935 – 954HelicalSequence analysisAdd BLAST20
Topological domaini955 – 984CytoplasmicSequence analysisAdd BLAST30
Transmembranei985 – 1006HelicalSequence analysisAdd BLAST22
Topological domaini1007 – 1013ExtracellularSequence analysis7
Transmembranei1014 – 1036HelicalSequence analysisAdd BLAST23
Topological domaini1037 – 1042CytoplasmicSequence analysis6
Transmembranei1043 – 1063HelicalSequence analysisAdd BLAST21
Topological domaini1064 – 1076ExtracellularSequence analysisAdd BLAST13
Transmembranei1077 – 1087HelicalSequence analysisAdd BLAST11
Topological domaini1088 – 1213CytoplasmicSequence analysisAdd BLAST126

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • endosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired growth of roots and shoots (PubMed:18344284, PubMed:23667493). Roots devoided of the characteristic trans-Golgi proliferation of slime vesicles containing polysaccharides and enzymes for secretion (PubMed:18344284). Aberrant trichome expansion, reduced primary root growth and longer root hairs (PubMed:19566596). Impaired pollen growth (PubMed:19566596, PubMed:23667493). Impaired ovule development (PubMed:23667493). Reduced adaptability to temperature stresses (PubMed:23667493).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi413D → A: Loss of internalization of phospholipids. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463871 – 1213Phospholipid-transporting ATPase 3Add BLAST1213

Proteomic databases

PaxDbiQ9XIE6.
PRIDEiQ9XIE6.

PTM databases

iPTMnetiQ9XIE6.

Expressioni

Tissue specificityi

Expressed in petals and sepals, but not in reproductive tissues. In siliques, detected in the upper part of the seed pod and in the area between the seed pod and the stem, but not in developing seeds. Strong expression in vascular shoot tissues and in stomatal guard cells of young rosettes leaves. In roots, expressed in cells surrounding the xylem and in central and peripheral columella cells. Detected in developing and mature trichomes, roots, pollen and growing pollen tubes.2 Publications

Gene expression databases

ExpressionAtlasiQ9XIE6. baseline and differential.
GenevisibleiQ9XIE6. AT.

Interactioni

Subunit structurei

Associates with ALIS1 to form a stable and active complex. Interacts with ALIS3 and ALIS5 in a heterologous system.2 Publications

Protein-protein interaction databases

BioGridi27500. 1 interactor.
STRINGi3702.AT1G59820.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XIE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000202528.
InParanoidiQ9XIE6.
KOiK14802.
OMAiWYIKKMD.
OrthoDBiEOG093600FE.
PhylomeDBiQ9XIE6.

Family and domain databases

Gene3Di3.40.1110.10. 2 hits.
3.40.50.1000. 1 hit.
InterProiView protein in InterPro
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiView protein in Pfam
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81653. SSF81653. 2 hits.
SSF81660. SSF81660. 2 hits.
SSF81665. SSF81665. 3 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiView protein in PROSITE
PS00154. ATPASE_E1_E2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9XIE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRSGSFSVD SSATHQRTPS RTVTLGHIQP QAPTYRTVYC NDRESNQPVR 
        60         70         80         90        100
FKGNSISTTK YNVFTFLPKG LFEQFRRIAN IYFLGISCLS MTPISPVSPI 
       110        120        130        140        150
TNVAPLSMVL LVSLIKEAFE DWKRFQNDMS INNSTVEILQ DQQWVSIPWR 
       160        170        180        190        200
KLQVGDIVKI KKDGFFPADI LFMSSTNSDG ICYVETANLD GETNLKIRKA 
       210        220        230        240        250
LERTWDYLVP EKAYEFKGEI QCEQPNNSLY TFTGNLVVQK QTLPLSPDQL 
       260        270        280        290        300
LLRGCSLRNT EYIVGAVVFT GHETKVMMNA MNAPSKRSTL EKKLDKLIIT 
       310        320        330        340        350
IFCVLVTMCL IGAIGCSIVT DREDKYLGLH NSDWEYRNGL MIGFFTFFTL 
       360        370        380        390        400
VTLFSSIIPI SLYVSIEMIK FIQSTQFINR DLNMYHAETN TPASARTSNL 
       410        420        430        440        450
NEELGQVEYI FSDKTGTLTR NLMEFFKCSI GGVSYGCGVT EIEKGIAQRH 
       460        470        480        490        500
GLKVQEEQRS TGAIREKGFN FDDPRLMRGA WRNEPNPDLC KELFRCLAIC 
       510        520        530        540        550
HTVLPEGDES PEKIVYQAAS PDEAALVTAA KNFGFFFYRR TPTMVYVRES 
       560        570        580        590        600
HVEKMGKIQD VAYEILNVLE FNSTRKRQSV VCRFPDGRLV LYCKGADNVI 
       610        620        630        640        650
FERLANGMDD VRKVTREHLE HFGSSGLRTL CLAYKDLNPE TYDSWNEKFI 
       660        670        680        690        700
QAKSALRDRE KKLDEVAELI EKDLILIGST AIEDKLQEGV PTCIETLSRA 
       710        720        730        740        750
GIKIWVLTGD KMETAINIAY ACNLINNEMK QFVISSETDA IREAEERGDQ 
       760        770        780        790        800
VEIARVIKEE VKRELKKSLE EAQHSLHTVA GPKLSLVIDG KCLMYALDPS 
       810        820        830        840        850
LRVMLLSLSL NCTSVVCCRV SPLQKAQVTS LVRKGAQKIT LSIGDGANDV 
       860        870        880        890        900
SMIQAAHVGI GISGMEGMQA VMASDFAIAQ FRFLTDLLLV HGRWSYLRIC 
       910        920        930        940        950
KVVMYFFYKN LTFTLTQFWF TFRTGFSGQR FYDDWFQSLF NVVFTALPVI 
       960        970        980        990       1000
VLGLFEKDVS ASLSKRYPEL YREGIRNSFF KWRVVAVWAT SAVYQSLVCY 
      1010       1020       1030       1040       1050
LFVTTSSFGA VNSSGKVFGL WDVSTMVFTC LVIAVNVRIL LMSNSITRWH 
      1060       1070       1080       1090       1100
YITVGGSILA WLVFAFVYCG IMTPHDRNEN VYFVIYVLMS TFYFYFTLLL 
      1110       1120       1130       1140       1150
VPIVSLLGDF IFQGVERWFF PYDYQIVQEI HRHESDASKA DQLEVENELT 
      1160       1170       1180       1190       1200
PQEARSYAIS QLPRELSKHT GFAFDSPGYE SFFASQLGIY APQKAWDVAR 
      1210 
RASMRSRPKV PKK
Length:1,213
Mass (Da):137,753
Last modified:February 28, 2003 - v2
Checksum:i938642DEFDA28B66
GO

Sequence cautioni

The sequence AAD39325 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007258 Genomic DNA. Translation: AAD39325.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33621.1.
AY091777 mRNA. Translation: AAM10325.1.
PIRiC96622.
RefSeqiNP_176191.1. NM_104675.3.
UniGeneiAt.36777.

Genome annotation databases

EnsemblPlantsiAT1G59820.1; AT1G59820.1; AT1G59820.
GeneIDi842275.
GrameneiAT1G59820.1; AT1G59820.1; AT1G59820.
KEGGiath:AT1G59820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007258 Genomic DNA. Translation: AAD39325.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33621.1.
AY091777 mRNA. Translation: AAM10325.1.
PIRiC96622.
RefSeqiNP_176191.1. NM_104675.3.
UniGeneiAt.36777.

3D structure databases

ProteinModelPortaliQ9XIE6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27500. 1 interactor.
STRINGi3702.AT1G59820.1.

Protein family/group databases

TCDBi3.A.3.8.6. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiQ9XIE6.

Proteomic databases

PaxDbiQ9XIE6.
PRIDEiQ9XIE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G59820.1; AT1G59820.1; AT1G59820.
GeneIDi842275.
GrameneiAT1G59820.1; AT1G59820.1; AT1G59820.
KEGGiath:AT1G59820.

Organism-specific databases

AraportiAT1G59820.
TAIRilocus:2025961. AT1G59820.

Phylogenomic databases

eggNOGiENOG410ITKD. Eukaryota.
ENOG410XPYK. LUCA.
HOGENOMiHOG000202528.
InParanoidiQ9XIE6.
KOiK14802.
OMAiWYIKKMD.
OrthoDBiEOG093600FE.
PhylomeDBiQ9XIE6.

Enzyme and pathway databases

BioCyciARA:AT1G59820-MONOMER.
BRENDAi3.6.99.B1. 399.
ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiPR:Q9XIE6.

Gene expression databases

ExpressionAtlasiQ9XIE6. baseline and differential.
GenevisibleiQ9XIE6. AT.

Family and domain databases

Gene3Di3.40.1110.10. 2 hits.
3.40.50.1000. 1 hit.
InterProiView protein in InterPro
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiView protein in Pfam
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81653. SSF81653. 2 hits.
SSF81660. SSF81660. 2 hits.
SSF81665. SSF81665. 3 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiView protein in PROSITE
PS00154. ATPASE_E1_E2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiALA3_ARATH
AccessioniPrimary (citable) accession number: Q9XIE6
Secondary accession number(s): Q8RWQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: February 28, 2003
Last modified: June 7, 2017
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.