Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Somatic embryogenesis receptor kinase 2

Gene

SERK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-kinase involved in brassinosteroid-dependent and -independent signaling pathways. Acts redundantly with SERK1 as a control point for sporophytic development controlling male gametophyte production.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei333 – 3331ATPPROSITE-ProRule annotation
Active sitei432 – 4321Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi311 – 3199ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: TAIR
  • microsporogenesis Source: TAIR
  • pollen maturation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G34210-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Somatic embryogenesis receptor kinase 2 (EC:2.7.11.1)
Short name:
AtSERK2
Alternative name(s):
Somatic embryogenesis receptor-like kinase 2
Gene namesi
Name:SERK2
Ordered Locus Names:At1g34210
ORF Names:F23M19.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G34210.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 241212ExtracellularSequence analysisAdd
BLAST
Transmembranei242 – 26221HelicalSequence analysisAdd
BLAST
Topological domaini263 – 628366CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Serk1 and serk2 double mutants are completely male sterile due to a failure in tapetum specification.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 628599Somatic embryogenesis receptor kinase 2PRO_0000380725Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence analysis
Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence analysis
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence analysis
Modified residuei302 – 3021Phosphothreonine1 Publication
Modified residuei328 – 3281PhosphothreonineBy similarity
Modified residuei386 – 3861PhosphoserineBy similarity
Modified residuei389 – 3891PhosphoserineBy similarity
Modified residuei462 – 4621Phosphothreonine1 Publication
Modified residuei465 – 4651Phosphothreonine1 Publication
Modified residuei466 – 4661Phosphothreonine1 Publication
Modified residuei471 – 4711PhosphothreonineBy similarity
Modified residuei479 – 4791PhosphotyrosineBy similarity
Modified residuei481 – 4811PhosphoserineBy similarity
Modified residuei482 – 4821PhosphothreonineBy similarity
Modified residuei486 – 4861PhosphoserineBy similarity
Modified residuei562 – 5621PhosphothreonineBy similarity
Modified residuei604 – 6041Phosphoserine1 Publication
Modified residuei616 – 6161Phosphothreonine1 Publication
Modified residuei625 – 6251Phosphoserine1 Publication

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9XIC7.
PRIDEiQ9XIC7.

PTM databases

iPTMnetiQ9XIC7.

Expressioni

Tissue specificityi

Expressed in flowers, tapetum, developing microspores, all cells of the embryo sac, provascular strands and developing vascular bundles. Low expression in adult vascular tissue.1 Publication

Gene expression databases

GenevisibleiQ9XIC7. AT.

Interactioni

Subunit structurei

Homo- and heterodimer. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1 (By similarity). Interacts with the EF-Tu receptor EFR and FLS2 in a specific ligand-induced manner.By similarity2 Publications

GO - Molecular functioni

  • receptor serine/threonine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi25552. 10 interactions.
DIPiDIP-61781N.
IntActiQ9XIC7. 1 interaction.
STRINGi3702.AT1G34210.1.

Structurei

Secondary structure

1
628
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 4212Combined sources
Turni49 – 524Combined sources
Beta strandi66 – 683Combined sources
Beta strandi74 – 785Combined sources
Beta strandi85 – 873Combined sources
Helixi90 – 945Combined sources
Beta strandi100 – 1023Combined sources
Helixi114 – 1185Combined sources
Beta strandi123 – 1264Combined sources
Helixi138 – 1425Combined sources
Beta strandi148 – 1503Combined sources
Helixi162 – 1665Combined sources
Beta strandi172 – 1743Combined sources
Beta strandi177 – 1837Combined sources
Helixi188 – 1925Combined sources
Helixi195 – 1984Combined sources
Beta strandi202 – 2065Combined sources
Beta strandi209 – 2113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4Z61X-ray2.75C/D1-216[»]
ProteinModelPortaliQ9XIC7.
SMRiQ9XIC7. Positions 30-215, 242-592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati97 – 12024LRR 1Add
BLAST
Repeati121 – 14323LRR 2Add
BLAST
Repeati145 – 16622LRR 3Add
BLAST
Repeati169 – 19123LRR 4Add
BLAST
Domaini305 – 592288Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi207 – 23428Pro-richAdd
BLAST
Compositional biasi239 – 26325Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGSH. Eukaryota.
ENOG410ZNK7. LUCA.
HOGENOMiHOG000116554.
InParanoidiQ9XIC7.
KOiK13418.
OMAiPQEYFFD.
OrthoDBiEOG093605SM.
PhylomeDBiQ9XIC7.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR031048. SERK.
[Graphical view]
PANTHERiPTHR27001:SF151. PTHR27001:SF151. 1 hit.
PfamiPF00560. LRR_1. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XIC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKKFEAFG FVCLISLLLL FNSLWLASSN MEGDALHSLR ANLVDPNNVL
60 70 80 90 100
QSWDPTLVNP CTWFHVTCNN ENSVIRVDLG NADLSGQLVP QLGQLKNLQY
110 120 130 140 150
LELYSNNITG PVPSDLGNLT NLVSLDLYLN SFTGPIPDSL GKLFKLRFLR
160 170 180 190 200
LNNNSLTGPI PMSLTNIMTL QVLDLSNNRL SGSVPDNGSF SLFTPISFAN
210 220 230 240 250
NLDLCGPVTS RPCPGSPPFS PPPPFIPPPI VPTPGGYSAT GAIAGGVAAG
260 270 280 290 300
AALLFAAPAL AFAWWRRRKP QEFFFDVPAE EDPEVHLGQL KRFSLRELQV
310 320 330 340 350
ATDSFSNKNI LGRGGFGKVY KGRLADGTLV AVKRLKEERT PGGELQFQTE
360 370 380 390 400
VEMISMAVHR NLLRLRGFCM TPTERLLVYP YMANGSVASC LRERPPSQLP
410 420 430 440 450
LAWSIRQQIA LGSARGLSYL HDHCDPKIIH RDVKAANILL DEEFEAVVGD
460 470 480 490 500
FGLARLMDYK DTHVTTAVRG TIGHIAPEYL STGKSSEKTD VFGYGIMLLE
510 520 530 540 550
LITGQRAFDL ARLANDDDVM LLDWVKGLLK EKKLEMLVDP DLQSNYTEAE
560 570 580 590 600
VEQLIQVALL CTQSSPMERP KMSEVVRMLE GDGLAEKWDE WQKVEVLRQE
610 620
VELSSHPTSD WILDSTDNLH AMELSGPR
Length:628
Mass (Da):69,403
Last modified:November 1, 1999 - v1
Checksum:i23F6C0DC3717C74F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471N → Y (PubMed:11706164).Curated
Sequence conflicti47 – 471N → Y (PubMed:11523644).Curated
Sequence conflicti47 – 471N → Y in AAK68073 (PubMed:16284305).Curated
Sequence conflicti168 – 1681M → T (PubMed:11706164).Curated
Sequence conflicti168 – 1681M → T (PubMed:11523644).Curated
Sequence conflicti168 – 1681M → T in AAK68073 (PubMed:16284305).Curated
Sequence conflicti544 – 5441S → G (PubMed:11706164).Curated
Sequence conflicti544 – 5441S → G (PubMed:11523644).Curated
Sequence conflicti544 – 5441S → G in AAK68073 (PubMed:16284305).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384969 mRNA. Translation: AAK68073.1.
FJ708645 mRNA. Translation: ACN59241.1.
AC007454 Genomic DNA. Translation: AAD39611.1.
CP002684 Genomic DNA. Translation: AEE31686.1.
AK229715 mRNA. Translation: BAF01553.1.
PIRiD86466.
RefSeqiNP_174683.1. NM_103144.4.
UniGeneiAt.11393.
At.48266.

Genome annotation databases

EnsemblPlantsiAT1G34210.1; AT1G34210.1; AT1G34210.
GeneIDi840320.
GrameneiAT1G34210.1; AT1G34210.1; AT1G34210.
KEGGiath:AT1G34210.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384969 mRNA. Translation: AAK68073.1.
FJ708645 mRNA. Translation: ACN59241.1.
AC007454 Genomic DNA. Translation: AAD39611.1.
CP002684 Genomic DNA. Translation: AEE31686.1.
AK229715 mRNA. Translation: BAF01553.1.
PIRiD86466.
RefSeqiNP_174683.1. NM_103144.4.
UniGeneiAt.11393.
At.48266.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4Z61X-ray2.75C/D1-216[»]
ProteinModelPortaliQ9XIC7.
SMRiQ9XIC7. Positions 30-215, 242-592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25552. 10 interactions.
DIPiDIP-61781N.
IntActiQ9XIC7. 1 interaction.
STRINGi3702.AT1G34210.1.

PTM databases

iPTMnetiQ9XIC7.

Proteomic databases

PaxDbiQ9XIC7.
PRIDEiQ9XIC7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G34210.1; AT1G34210.1; AT1G34210.
GeneIDi840320.
GrameneiAT1G34210.1; AT1G34210.1; AT1G34210.
KEGGiath:AT1G34210.

Organism-specific databases

TAIRiAT1G34210.

Phylogenomic databases

eggNOGiENOG410IGSH. Eukaryota.
ENOG410ZNK7. LUCA.
HOGENOMiHOG000116554.
InParanoidiQ9XIC7.
KOiK13418.
OMAiPQEYFFD.
OrthoDBiEOG093605SM.
PhylomeDBiQ9XIC7.

Enzyme and pathway databases

BioCyciARA:AT1G34210-MONOMER.

Miscellaneous databases

PROiQ9XIC7.

Gene expression databases

GenevisibleiQ9XIC7. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR031048. SERK.
[Graphical view]
PANTHERiPTHR27001:SF151. PTHR27001:SF151. 1 hit.
PfamiPF00560. LRR_1. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSERK2_ARATH
AccessioniPrimary (citable) accession number: Q9XIC7
Secondary accession number(s): Q94F63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.