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Protein

Somatic embryogenesis receptor kinase 2

Gene

SERK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-kinase involved in brassinosteroid-dependent and -independent signaling pathways. Acts redundantly with SERK1 as a control point for sporophytic development controlling male gametophyte production.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei333ATPPROSITE-ProRule annotation1
Active sitei432Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi311 – 319ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: TAIR
  • microsporogenesis Source: TAIR
  • pollen maturation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Somatic embryogenesis receptor kinase 2 (EC:2.7.11.1)
Short name:
AtSERK2
Alternative name(s):
Somatic embryogenesis receptor-like kinase 2
Gene namesi
Name:SERK2
Ordered Locus Names:At1g34210
ORF Names:F23M19.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G34210.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 241ExtracellularSequence analysisAdd BLAST212
Transmembranei242 – 262HelicalSequence analysisAdd BLAST21
Topological domaini263 – 628CytoplasmicSequence analysisAdd BLAST366

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Serk1 and serk2 double mutants are completely male sterile due to a failure in tapetum specification.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000038072530 – 628Somatic embryogenesis receptor kinase 2Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi107N-linked (GlcNAc...)Sequence analysis1
Glycosylationi118N-linked (GlcNAc...)Sequence analysis1
Glycosylationi153N-linked (GlcNAc...)Sequence analysis1
Glycosylationi187N-linked (GlcNAc...)Sequence analysis1
Modified residuei302Phosphothreonine1 Publication1
Modified residuei328PhosphothreonineBy similarity1
Modified residuei386PhosphoserineBy similarity1
Modified residuei389PhosphoserineBy similarity1
Modified residuei462Phosphothreonine1 Publication1
Modified residuei465Phosphothreonine1 Publication1
Modified residuei466Phosphothreonine1 Publication1
Modified residuei471PhosphothreonineBy similarity1
Modified residuei479PhosphotyrosineBy similarity1
Modified residuei481PhosphoserineBy similarity1
Modified residuei482PhosphothreonineBy similarity1
Modified residuei486PhosphoserineBy similarity1
Modified residuei562PhosphothreonineBy similarity1
Modified residuei604Phosphoserine1 Publication1
Modified residuei616Phosphothreonine1 Publication1
Modified residuei625Phosphoserine1 Publication1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9XIC7.
PRIDEiQ9XIC7.

PTM databases

iPTMnetiQ9XIC7.

Expressioni

Tissue specificityi

Expressed in flowers, tapetum, developing microspores, all cells of the embryo sac, provascular strands and developing vascular bundles. Low expression in adult vascular tissue.1 Publication

Gene expression databases

GenevisibleiQ9XIC7. AT.

Interactioni

Subunit structurei

Homo- and heterodimer. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1 (By similarity). Interacts with the EF-Tu receptor EFR and FLS2 in a specific ligand-induced manner.By similarity2 Publications

GO - Molecular functioni

  • receptor serine/threonine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi25552. 10 interactors.
DIPiDIP-61781N.
IntActiQ9XIC7. 1 interactor.
STRINGi3702.AT1G34210.1.

Structurei

Secondary structure

1628
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 42Combined sources12
Turni49 – 52Combined sources4
Beta strandi66 – 68Combined sources3
Beta strandi74 – 78Combined sources5
Beta strandi85 – 87Combined sources3
Helixi90 – 94Combined sources5
Beta strandi100 – 102Combined sources3
Helixi114 – 118Combined sources5
Beta strandi123 – 126Combined sources4
Helixi138 – 142Combined sources5
Beta strandi148 – 150Combined sources3
Helixi162 – 166Combined sources5
Beta strandi172 – 174Combined sources3
Beta strandi177 – 183Combined sources7
Helixi188 – 192Combined sources5
Helixi195 – 198Combined sources4
Beta strandi202 – 206Combined sources5
Beta strandi209 – 211Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z61X-ray2.75C/D1-216[»]
ProteinModelPortaliQ9XIC7.
SMRiQ9XIC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati97 – 120LRR 1Add BLAST24
Repeati121 – 143LRR 2Add BLAST23
Repeati145 – 166LRR 3Add BLAST22
Repeati169 – 191LRR 4Add BLAST23
Domaini305 – 592Protein kinasePROSITE-ProRule annotationAdd BLAST288

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi207 – 234Pro-richAdd BLAST28
Compositional biasi239 – 263Ala-richAdd BLAST25

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGSH. Eukaryota.
ENOG410ZNK7. LUCA.
HOGENOMiHOG000116554.
InParanoidiQ9XIC7.
KOiK13418.
OMAiPQEYFFD.
OrthoDBiEOG093605SM.
PhylomeDBiQ9XIC7.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR031048. SERK.
[Graphical view]
PANTHERiPTHR27001:SF151. PTHR27001:SF151. 1 hit.
PfamiPF00560. LRR_1. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XIC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKKFEAFG FVCLISLLLL FNSLWLASSN MEGDALHSLR ANLVDPNNVL
60 70 80 90 100
QSWDPTLVNP CTWFHVTCNN ENSVIRVDLG NADLSGQLVP QLGQLKNLQY
110 120 130 140 150
LELYSNNITG PVPSDLGNLT NLVSLDLYLN SFTGPIPDSL GKLFKLRFLR
160 170 180 190 200
LNNNSLTGPI PMSLTNIMTL QVLDLSNNRL SGSVPDNGSF SLFTPISFAN
210 220 230 240 250
NLDLCGPVTS RPCPGSPPFS PPPPFIPPPI VPTPGGYSAT GAIAGGVAAG
260 270 280 290 300
AALLFAAPAL AFAWWRRRKP QEFFFDVPAE EDPEVHLGQL KRFSLRELQV
310 320 330 340 350
ATDSFSNKNI LGRGGFGKVY KGRLADGTLV AVKRLKEERT PGGELQFQTE
360 370 380 390 400
VEMISMAVHR NLLRLRGFCM TPTERLLVYP YMANGSVASC LRERPPSQLP
410 420 430 440 450
LAWSIRQQIA LGSARGLSYL HDHCDPKIIH RDVKAANILL DEEFEAVVGD
460 470 480 490 500
FGLARLMDYK DTHVTTAVRG TIGHIAPEYL STGKSSEKTD VFGYGIMLLE
510 520 530 540 550
LITGQRAFDL ARLANDDDVM LLDWVKGLLK EKKLEMLVDP DLQSNYTEAE
560 570 580 590 600
VEQLIQVALL CTQSSPMERP KMSEVVRMLE GDGLAEKWDE WQKVEVLRQE
610 620
VELSSHPTSD WILDSTDNLH AMELSGPR
Length:628
Mass (Da):69,403
Last modified:November 1, 1999 - v1
Checksum:i23F6C0DC3717C74F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47N → Y (PubMed:11706164).Curated1
Sequence conflicti47N → Y (PubMed:11523644).Curated1
Sequence conflicti47N → Y in AAK68073 (PubMed:16284305).Curated1
Sequence conflicti168M → T (PubMed:11706164).Curated1
Sequence conflicti168M → T (PubMed:11523644).Curated1
Sequence conflicti168M → T in AAK68073 (PubMed:16284305).Curated1
Sequence conflicti544S → G (PubMed:11706164).Curated1
Sequence conflicti544S → G (PubMed:11523644).Curated1
Sequence conflicti544S → G in AAK68073 (PubMed:16284305).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384969 mRNA. Translation: AAK68073.1.
FJ708645 mRNA. Translation: ACN59241.1.
AC007454 Genomic DNA. Translation: AAD39611.1.
CP002684 Genomic DNA. Translation: AEE31686.1.
AK229715 mRNA. Translation: BAF01553.1.
PIRiD86466.
RefSeqiNP_174683.1. NM_103144.5.
UniGeneiAt.11393.
At.48266.

Genome annotation databases

EnsemblPlantsiAT1G34210.1; AT1G34210.1; AT1G34210.
GeneIDi840320.
GrameneiAT1G34210.1; AT1G34210.1; AT1G34210.
KEGGiath:AT1G34210.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF384969 mRNA. Translation: AAK68073.1.
FJ708645 mRNA. Translation: ACN59241.1.
AC007454 Genomic DNA. Translation: AAD39611.1.
CP002684 Genomic DNA. Translation: AEE31686.1.
AK229715 mRNA. Translation: BAF01553.1.
PIRiD86466.
RefSeqiNP_174683.1. NM_103144.5.
UniGeneiAt.11393.
At.48266.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z61X-ray2.75C/D1-216[»]
ProteinModelPortaliQ9XIC7.
SMRiQ9XIC7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25552. 10 interactors.
DIPiDIP-61781N.
IntActiQ9XIC7. 1 interactor.
STRINGi3702.AT1G34210.1.

PTM databases

iPTMnetiQ9XIC7.

Proteomic databases

PaxDbiQ9XIC7.
PRIDEiQ9XIC7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G34210.1; AT1G34210.1; AT1G34210.
GeneIDi840320.
GrameneiAT1G34210.1; AT1G34210.1; AT1G34210.
KEGGiath:AT1G34210.

Organism-specific databases

TAIRiAT1G34210.

Phylogenomic databases

eggNOGiENOG410IGSH. Eukaryota.
ENOG410ZNK7. LUCA.
HOGENOMiHOG000116554.
InParanoidiQ9XIC7.
KOiK13418.
OMAiPQEYFFD.
OrthoDBiEOG093605SM.
PhylomeDBiQ9XIC7.

Miscellaneous databases

PROiQ9XIC7.

Gene expression databases

GenevisibleiQ9XIC7. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR031048. SERK.
[Graphical view]
PANTHERiPTHR27001:SF151. PTHR27001:SF151. 1 hit.
PfamiPF00560. LRR_1. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSERK2_ARATH
AccessioniPrimary (citable) accession number: Q9XIC7
Secondary accession number(s): Q94F63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.