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Q9XIA9

- LACS2_ARATH

UniProt

Q9XIA9 - LACS2_ARATH

Protein

Long chain acyl-CoA synthetase 2

Gene

LACS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in the cutin pathway. Preferentially uses palmitate, palmitoleate, oleate and linoleate. Required for repression of lateral root formation through its role in cutin biosynthesis and subsequent aerial tissues permeability.5 Publications

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi228 – 23912ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: UniProtKB
    3. very long-chain fatty acid-CoA ligase activity Source: TAIR

    GO - Biological processi

    1. cutin biosynthetic process Source: TAIR
    2. defense response to fungus Source: TAIR
    3. fatty acid metabolic process Source: UniProtKB
    4. lateral root formation Source: TAIR
    5. wax biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G49430-MONOMER.
    MetaCyc:AT1G49430-MONOMER.
    SABIO-RKQ9XIA9.
    UniPathwayiUPA00199.

    Protein family/group databases

    TCDBi4.C.1.1.14. the proposed fatty acid transporter (fat) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long chain acyl-CoA synthetase 2 (EC:6.2.1.3)
    Alternative name(s):
    Protein Botrytis resistant 1
    Protein LATERAL ROOT DEVELOPMENT 2
    Gene namesi
    Name:LACS2
    Synonyms:BRE1, LRD2, SMA4
    Ordered Locus Names:At1g49430
    ORF Names:F13F21.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G49430.

    Subcellular locationi

    Endoplasmic reticulum 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Disruption phenotypei

    Dwarf phenotype with smaller, wrinkled leaves and overall reduced vigor. Higher water loss rate and susceptibility to drought stress. Defective in the cuticular membrane. Strong resistance to virulent Botrytis cinerea and enhanced susceptibility to avirulent Pseudomonas syringae.4 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 665665Long chain acyl-CoA synthetase 2PRO_0000401410Add
    BLAST

    Proteomic databases

    PaxDbiQ9XIA9.
    PRIDEiQ9XIA9.

    Expressioni

    Tissue specificityi

    Expressed along the entire length of the stem, but expression was not entirely epidermal specific, with some expression found in internal cell layers as well. Was expressed in leave epidermal cells, flowers (sepals, petals, stamens, filaments and carpel), siliques and developing seeds. In roots, expression was detected in an internal cell layer, probably the endodermal layer.2 Publications

    Inductioni

    Positively regulated by WIN1.1 Publication

    Gene expression databases

    GenevestigatoriQ9XIA9.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G49430.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XIA9.
    SMRiQ9XIA9. Positions 48-579.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni496 – 52025Fatty acid-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    HOGENOMiHOG000159459.
    InParanoidiQ9XIA9.
    KOiK01897.
    OMAiCNTLSNR.
    PhylomeDBiQ9XIA9.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XIA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLAADNVLL VEEGRPATAE HPSAGPVYRC KYAKDGLLDL PTDIDSPWQF    50
    FSEAVKKYPN EQMLGQRVTT DSKVGPYTWI TYKEAHDAAI RIGSAIRSRG 100
    VDPGHCCGIY GANCPEWIIA MEACMSQGIT YVPLYDSLGV NAVEFIINHA 150
    EVSLVFVQEK TVSSILSCQK GCSSNLKTIV SFGEVSSTQK EEAKNQCVSL 200
    FSWNEFSLMG NLDEANLPRK RKTDICTIMY TSGTTGEPKG VILNNAAISV 250
    QVLSIDKMLE VTDRSCDTSD VFFSYLPLAH CYDQVMEIYF LSRGSSVGYW 300
    RGDIRYLMDD VQALKPTVFC GVPRVYDKLY AGIMQKISAS GLIRKKLFDF 350
    AYNYKLGNMR KGFSQEEASP RLDRLMFDKI KEALGGRAHM LLSGAAPLPR 400
    HVEEFLRIIP ASNLSQGYGL TESCGGSFTT LAGVFSMVGT VGVPMPTVEA 450
    RLVSVPEMGY DAFSADVPRG EICLRGNSMF SGYHKRQDLT DQVLIDGWFH 500
    TGDIGEWQED GSMKIIDRKK NIFKLSQGEY VAVENLENTY SRCPLIAQIW 550
    VYGNSFESFL VGVVVPDRKA IEDWAKLNYQ SPNDFESLCQ NLKAQKYFLD 600
    ELNSTAKQYQ LKGFEMLKAI HLEPNPFDIE RDLITPTFKL KRPQLLQHYK 650
    GIVDQLYSEA KRSMA 665
    Length:665
    Mass (Da):74,389
    Last modified:November 1, 1999 - v1
    Checksum:i9B1566F79A1B2D1A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503752 mRNA. Translation: AAM28869.1.
    AC007504 Genomic DNA. Translation: AAD43157.1.
    CP002684 Genomic DNA. Translation: AEE32429.1.
    AY065424 mRNA. Translation: AAL38865.1.
    AY094420 mRNA. Translation: AAM19793.1.
    BT001970 mRNA. Translation: AAN71969.1.
    PIRiG96530.
    RefSeqiNP_175368.2. NM_103833.4.
    UniGeneiAt.27757.

    Genome annotation databases

    EnsemblPlantsiAT1G49430.1; AT1G49430.1; AT1G49430.
    GeneIDi841367.
    KEGGiath:AT1G49430.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503752 mRNA. Translation: AAM28869.1 .
    AC007504 Genomic DNA. Translation: AAD43157.1 .
    CP002684 Genomic DNA. Translation: AEE32429.1 .
    AY065424 mRNA. Translation: AAL38865.1 .
    AY094420 mRNA. Translation: AAM19793.1 .
    BT001970 mRNA. Translation: AAN71969.1 .
    PIRi G96530.
    RefSeqi NP_175368.2. NM_103833.4.
    UniGenei At.27757.

    3D structure databases

    ProteinModelPortali Q9XIA9.
    SMRi Q9XIA9. Positions 48-579.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G49430.1-P.

    Protein family/group databases

    TCDBi 4.C.1.1.14. the proposed fatty acid transporter (fat) family.

    Proteomic databases

    PaxDbi Q9XIA9.
    PRIDEi Q9XIA9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G49430.1 ; AT1G49430.1 ; AT1G49430 .
    GeneIDi 841367.
    KEGGi ath:AT1G49430.

    Organism-specific databases

    TAIRi AT1G49430.

    Phylogenomic databases

    eggNOGi COG1022.
    HOGENOMi HOG000159459.
    InParanoidi Q9XIA9.
    KOi K01897.
    OMAi CNTLSNR.
    PhylomeDBi Q9XIA9.

    Enzyme and pathway databases

    UniPathwayi UPA00199 .
    BioCyci ARA:AT1G49430-MONOMER.
    MetaCyc:AT1G49430-MONOMER.
    SABIO-RK Q9XIA9.

    Gene expression databases

    Genevestigatori Q9XIA9.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
      Shockey J.M., Fulda M.S., Browse J.A.
      Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
      Shockey J.M., Fulda M.S., Browse J.
      Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION.
    6. "The acyl-CoA synthetase encoded by LACS2 is essential for normal cuticle development in Arabidopsis."
      Schnurr J., Shockey J., Browse J.
      Plant Cell 16:629-642(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    7. "Osmotic regulation of root system architecture."
      Deak K.I., Malamy J.
      Plant J. 43:17-28(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "A permeable cuticle in Arabidopsis leads to a strong resistance to Botrytis cinerea."
      Bessire M., Chassot C., Jacquat A.C., Humphry M., Borel S., Petetot J.M., Metraux J.P., Nawrath C.
      EMBO J. 26:2158-2168(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    9. "The transcription factor WIN1/SHN1 regulates Cutin biosynthesis in Arabidopsis thaliana."
      Kannangara R., Branigan C., Liu Y., Penfield T., Rao V., Mouille G., Hoefte H., Pauly M., Riechmann J.L., Broun P.
      Plant Cell 19:1278-1294(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY WIN1.
    10. "Mutations in LACS2, a long-chain acyl-coenzyme A synthetase, enhance susceptibility to avirulent Pseudomonas syringae but confer resistance to Botrytis cinerea in Arabidopsis."
      Tang D., Simonich M.T., Innes R.W.
      Plant Physiol. 144:1093-1103(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    11. "Root system architecture in Arabidopsis grown in culture is regulated by sucrose uptake in the aerial tissues."
      Macgregor D.R., Deak K.I., Ingram P.A., Malamy J.E.
      Plant Cell 20:2643-2660(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis."
      Lue S., Song T., Kosma D.K., Parsons E.P., Rowland O., Jenks M.A.
      Plant J. 59:553-564(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    13. "Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis."
      Weng H., Molina I., Shockey J., Browse J.
      Planta 231:1089-1100(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiLACS2_ARATH
    AccessioniPrimary (citable) accession number: Q9XIA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3