Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9XI91

- IF5A1_ARATH

UniProt

Q9XI91 - IF5A1_ARATH

Protein

Eukaryotic translation initiation factor 5A-1

Gene

ELF5A-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The precise role of eIF-5A in protein biosynthesis is not known but it may function as a bimodular protein capable of binding to both RNA and proteins. Involved in xylogenesis.1 Publication

    GO - Molecular functioni

    1. ribosome binding Source: InterPro
    2. translation elongation factor activity Source: InterPro
    3. translation initiation factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. peptidyl-lysine modification to peptidyl-hypusine Source: InterPro
    2. positive regulation of translational elongation Source: InterPro
    3. positive regulation of translational termination Source: InterPro
    4. translational frameshifting Source: InterPro
    5. xylem development Source: TAIR

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 5A-1
    Short name:
    AtELF5A-1
    Short name:
    eIF-5A-1
    Gene namesi
    Name:ELF5A-1
    Ordered Locus Names:At1g13950
    ORF Names:F16A14.17, F7A19.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G13950.

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype, but presence of extra root protoxylem cell files.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 158158Eukaryotic translation initiation factor 5A-1PRO_0000142463Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21PhosphoserineBy similarity
    Modified residuei51 – 511HypusineCurated

    Post-translational modificationi

    eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

    Keywords - PTMi

    Hypusine, Phosphoprotein

    Proteomic databases

    PaxDbiQ9XI91.
    PRIDEiQ9XI91.

    Expressioni

    Tissue specificityi

    Expressed in leaf vasculature and inflorescence stems. Present in xylem tissue but not in phloem, and in developing vessel members, but not in mature vessels members. Detected in anthers.3 Publications

    Developmental stagei

    Increases at the onset of leaf senescence.1 Publication

    Inductioni

    Up-regulated at post-transcriptional level by iron deficiency.2 Publications

    Gene expression databases

    GenevestigatoriQ9XI91.

    Interactioni

    Protein-protein interaction databases

    BioGridi23195. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XI91.
    SMRiQ9XI91. Positions 16-156.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eIF-5A family.Curated

    Phylogenomic databases

    eggNOGiCOG0231.
    HOGENOMiHOG000106270.
    InParanoidiQ9XI91.
    KOiK03263.
    OMAiLMENGNT.
    PhylomeDBiQ9XI91.

    Family and domain databases

    Gene3Di2.30.30.30. 1 hit.
    2.40.50.140. 1 hit.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR014722. Rib_L2_dom2.
    IPR019769. Trans_elong_IF5A_hypusine_site.
    IPR001884. Transl_elong_IF5A.
    IPR020189. Transl_elong_IF5A_C.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view]
    PANTHERiPTHR11673. PTHR11673. 1 hit.
    PfamiPF01287. eIF-5a. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003025. eIF5A. 1 hit.
    SUPFAMiSSF50104. SSF50104. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
    PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XI91-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDEEHHFES SDAGASKTYP QQAGTIRKNG YIVIKNRPCK VVEVSTSKTG    50
    KHGHAKCHFV AIDIFTSKKL EDIVPSSHNC DVPHVNRTDY QLIDISEDGY 100
    VSLLTDNGST KDDLKLPNDD TLLQQIKSGF DDGKDLVVSV MSAMGEEQIN 150
    ALKDIGPK 158
    Length:158
    Mass (Da):17,360
    Last modified:November 1, 1999 - v1
    Checksum:iDD837BD71E2DEC7D
    GO

    Sequence cautioni

    The sequence AAF79401.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296082 mRNA. Translation: AAG53646.1.
    AC007576 Genomic DNA. Translation: AAD39281.1.
    AC068197 Genomic DNA. Translation: AAF79401.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29088.1.
    AY063780 mRNA. Translation: AAL36087.1.
    AY117272 mRNA. Translation: AAM51347.1.
    PIRiF86272.
    RefSeqiNP_172848.1. NM_101261.2.
    UniGeneiAt.24908.

    Genome annotation databases

    EnsemblPlantsiAT1G13950.1; AT1G13950.1; AT1G13950.
    GeneIDi837955.
    KEGGiath:AT1G13950.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296082 mRNA. Translation: AAG53646.1 .
    AC007576 Genomic DNA. Translation: AAD39281.1 .
    AC068197 Genomic DNA. Translation: AAF79401.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29088.1 .
    AY063780 mRNA. Translation: AAL36087.1 .
    AY117272 mRNA. Translation: AAM51347.1 .
    PIRi F86272.
    RefSeqi NP_172848.1. NM_101261.2.
    UniGenei At.24908.

    3D structure databases

    ProteinModelPortali Q9XI91.
    SMRi Q9XI91. Positions 16-156.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 23195. 1 interaction.

    Proteomic databases

    PaxDbi Q9XI91.
    PRIDEi Q9XI91.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G13950.1 ; AT1G13950.1 ; AT1G13950 .
    GeneIDi 837955.
    KEGGi ath:AT1G13950.

    Organism-specific databases

    TAIRi AT1G13950.

    Phylogenomic databases

    eggNOGi COG0231.
    HOGENOMi HOG000106270.
    InParanoidi Q9XI91.
    KOi K03263.
    OMAi LMENGNT.
    PhylomeDBi Q9XI91.

    Gene expression databases

    Genevestigatori Q9XI91.

    Family and domain databases

    Gene3Di 2.30.30.30. 1 hit.
    2.40.50.140. 1 hit.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR014722. Rib_L2_dom2.
    IPR019769. Trans_elong_IF5A_hypusine_site.
    IPR001884. Transl_elong_IF5A.
    IPR020189. Transl_elong_IF5A_C.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view ]
    PANTHERi PTHR11673. PTHR11673. 1 hit.
    Pfami PF01287. eIF-5a. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003025. eIF5A. 1 hit.
    SUPFAMi SSF50104. SSF50104. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00037. eIF_5A. 1 hit.
    PROSITEi PS00302. IF5A_HYPUSINE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato."
      Wang T.-W., Lu L., Wang D., Thompson J.E.
      J. Biol. Chem. 276:17541-17549(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], HYPUSINE.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects."
      Duguay J., Jamal S., Liu Z., Wang T.W., Thompson J.E.
      J. Plant Physiol. 164:408-420(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Modulation of eIF5A1 expression alters xylem abundance in Arabidopsis thaliana."
      Liu Z., Duguay J., Ma F., Wang T.W., Tshin R., Hopkins M.T., McNamara L., Thompson J.E.
      J. Exp. Bot. 59:939-950(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    7. "Arabidopsis eIF5A3 influences growth and the response to osmotic and nutrient stress."
      Ma F., Liu Z., Wang T.W., Hopkins M.T., Peterson C.A., Thompson J.E.
      Plant Cell Environ. 33:1682-1696(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    8. "iTRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for iron homeostasis."
      Lan P., Li W., Wen T.N., Shiau J.Y., Wu Y.C., Lin W., Schmidt W.
      Plant Physiol. 155:821-834(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY IRON DEFICIENCY.
    9. "The enigma of eIF5A in the iron deficiency response of Arabidopsis."
      Lan P., Schmidt W.
      Plant Signal. Behav. 6:528-530(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY IRON DEFICIENCY.
    10. "The Arabidopsis eukaryotic translation initiation factor eIF5A-2 regulates root protoxylem development by modulating cytokinin signaling."
      Ren B., Chen Q., Hong S., Zhao W., Feng J., Feng H., Zuo J.
      Plant Cell 25:3841-3857(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiIF5A1_ARATH
    AccessioniPrimary (citable) accession number: Q9XI91
    Secondary accession number(s): Q9LMG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2002
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Translation initiation factors
      List of translation initiation factor entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3