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Q9XI91 (IF5A1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 5A-1

Short name=AtELF5A-1
Short name=eIF-5A-1
Gene names
Name:ELF5A-1
Ordered Locus Names:At1g13950
ORF Names:F16A14.17, F7A19.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The precise role of eIF-5A in protein biosynthesis is not known but it may function as a bimodular protein capable of binding to both RNA and proteins. Involved in xylogenesis. Ref.6

Tissue specificity

Expressed in leaf vasculature and inflorescence stems. Present in xylem tissue but not in phloem, and in developing vessel members, but not in mature vessels members. Detected in anthers. Ref.5 Ref.6 Ref.10

Developmental stage

Increases at the onset of leaf senescence. Ref.7

Induction

Up-regulated at post-transcriptional level by iron deficiency. Ref.8 Ref.9

Post-translational modification

eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Disruption phenotype

No visible phenotype, but presence of extra root protoxylem cell files. Ref.10

Sequence similarities

Belongs to the eIF-5A family.

Sequence caution

The sequence AAF79401.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158Eukaryotic translation initiation factor 5A-1
PRO_0000142463

Amino acid modifications

Modified residue21Phosphoserine By similarity
Modified residue511Hypusine Probable

Sequences

Sequence LengthMass (Da)Tools
Q9XI91 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: DD837BD71E2DEC7D

FASTA15817,360
        10         20         30         40         50         60 
MSDEEHHFES SDAGASKTYP QQAGTIRKNG YIVIKNRPCK VVEVSTSKTG KHGHAKCHFV 

        70         80         90        100        110        120 
AIDIFTSKKL EDIVPSSHNC DVPHVNRTDY QLIDISEDGY VSLLTDNGST KDDLKLPNDD 

       130        140        150 
TLLQQIKSGF DDGKDLVVSV MSAMGEEQIN ALKDIGPK 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato."
Wang T.-W., Lu L., Wang D., Thompson J.E.
J. Biol. Chem. 276:17541-17549(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], HYPUSINE.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects."
Duguay J., Jamal S., Liu Z., Wang T.W., Thompson J.E.
J. Plant Physiol. 164:408-420(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Modulation of eIF5A1 expression alters xylem abundance in Arabidopsis thaliana."
Liu Z., Duguay J., Ma F., Wang T.W., Tshin R., Hopkins M.T., McNamara L., Thompson J.E.
J. Exp. Bot. 59:939-950(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]"Arabidopsis eIF5A3 influences growth and the response to osmotic and nutrient stress."
Ma F., Liu Z., Wang T.W., Hopkins M.T., Peterson C.A., Thompson J.E.
Plant Cell Environ. 33:1682-1696(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[8]"iTRAQ protein profile analysis of Arabidopsis roots reveals new aspects critical for iron homeostasis."
Lan P., Li W., Wen T.N., Shiau J.Y., Wu Y.C., Lin W., Schmidt W.
Plant Physiol. 155:821-834(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY IRON DEFICIENCY.
[9]"The enigma of eIF5A in the iron deficiency response of Arabidopsis."
Lan P., Schmidt W.
Plant Signal. Behav. 6:528-530(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY IRON DEFICIENCY.
[10]"The Arabidopsis eukaryotic translation initiation factor eIF5A-2 regulates root protoxylem development by modulating cytokinin signaling."
Ren B., Chen Q., Hong S., Zhao W., Feng J., Feng H., Zuo J.
Plant Cell 25:3841-3857(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF296082 mRNA. Translation: AAG53646.1.
AC007576 Genomic DNA. Translation: AAD39281.1.
AC068197 Genomic DNA. Translation: AAF79401.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29088.1.
AY063780 mRNA. Translation: AAL36087.1.
AY117272 mRNA. Translation: AAM51347.1.
PIRF86272.
RefSeqNP_172848.1. NM_101261.2.
UniGeneAt.24908.

3D structure databases

ProteinModelPortalQ9XI91.
SMRQ9XI91. Positions 16-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid23195. 1 interaction.

Proteomic databases

PaxDbQ9XI91.
PRIDEQ9XI91.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G13950.1; AT1G13950.1; AT1G13950.
GeneID837955.
KEGGath:AT1G13950.

Organism-specific databases

TAIRAT1G13950.

Phylogenomic databases

eggNOGCOG0231.
HOGENOMHOG000106270.
InParanoidQ9XI91.
KOK03263.
OMALMENGNT.
PhylomeDBQ9XI91.

Gene expression databases

GenevestigatorQ9XI91.

Family and domain databases

Gene3D2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
InterProIPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERPTHR11673. PTHR11673. 1 hit.
PfamPF01287. eIF-5a. 1 hit.
[Graphical view]
PIRSFPIRSF003025. eIF5A. 1 hit.
SUPFAMSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00037. eIF_5A. 1 hit.
PROSITEPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF5A1_ARATH
AccessionPrimary (citable) accession number: Q9XI91
Secondary accession number(s): Q9LMG2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names