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Q9XI62

- APY3_ARATH

UniProt

Q9XI62 - APY3_ARATH

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Protein

Probable apyrase 3

Gene

APY3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.By similarity

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Calcium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei195 – 1951Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 8211ATP-bindingCuratedAdd
BLAST
Nucleotide bindingi219 – 22911ATP-bindingCuratedAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G14240-MONOMER.
ARA:GQT-911-MONOMER.
ARA:GQT-912-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable apyrase 3 (EC:3.6.1.5)
Short name:
AtAPY3
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 3
Gene namesi
Name:APY3
Ordered Locus Names:At1g14240
ORF Names:F7A19.34
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G14240.

Subcellular locationi

Membrane By similarity; Single-pass type II membrane protein By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Probable apyrase 3PRO_0000420341Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9XI62.
PRIDEiQ9XI62.

Expressioni

Tissue specificityi

Expressed in the initiation zone of lateral root and in the lateral root tip, the adaxial junction of lateral shoots with the stems, and in the abscission zone of flower organs. Not expressed in the rosette leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ9XI62. baseline and differential.
GenevestigatoriQ9XI62.

Structurei

3D structure databases

ProteinModelPortaliQ9XI62.
SMRiQ9XI62. Positions 68-460.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini51 – 483433ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei30 – 5021Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000077482.
InParanoidiQ9XI62.
KOiK01510.
OMAiYLKTYCF.
PhylomeDBiQ9XI62.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9XI62) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTPETDALKV QILPKHQSLP YTVTKAKSKS LILLVVVSVT ITLGLLLYVF
60 70 80 90 100
NSNSVISSGS LLSRRCKLRY SVLIDAGSSG TRVHVFGYWF ESGKPVFDFG
110 120 130 140 150
EKHYANLKLT PGLSSYADNP EGASVSVTKL VEFAKQRIPK RMFRRSDIRL
160 170 180 190 200
MATAGMRLLE VPVQEQILEV TRRVLRSSGF MFRDEWANVI SGSDEGIYSW
210 220 230 240 250
ITANYALGSL GTDPLETTGI VELGGASAQV TFVSSEHVPP EYSRTIAYGN
260 270 280 290 300
ISYTIYSHSF LDYGKDAALK KLLEKLQNSA NSTVDGVVED PCTPKGYIYD
310 320 330 340 350
TNSKNYSSGF LADESKLKGS LQAAGNFSKC RSATFALLKE GKENCLYEHC
360 370 380 390 400
SIGSTFTPDL QGSFLATASF YYTAKFFELE EKGWLSELIP AGKRYCGEEW
410 420 430 440 450
SKLILEYPTT DEEYLRGYCF SAAYTISMLH DSLGIALDDE SITYASKAGE
460 470 480
KHIPLDWALG AFILDVVTPN SDYNGKSRKY LGF
Length:483
Mass (Da):53,426
Last modified:November 1, 1999 - v1
Checksum:i4E31D13830F1C8F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JF830008 mRNA. Translation: AEJ38084.1.
AC007576 Genomic DNA. Translation: AAD39311.1.
CP002684 Genomic DNA. Translation: AEE29127.1.
CP002684 Genomic DNA. Translation: AEE29128.1.
CP002684 Genomic DNA. Translation: AEE29130.1.
BT006000 mRNA. Translation: AAO64935.1.
AK227399 mRNA. Translation: BAE99403.1.
AK317332 mRNA. Translation: BAH20006.1.
PIRiD86276.
RefSeqiNP_001117284.1. NM_001123812.1. [Q9XI62-1]
NP_172876.1. NM_101290.4. [Q9XI62-1]
NP_973822.2. NM_202093.3. [Q9XI62-1]
UniGeneiAt.41982.

Genome annotation databases

EnsemblPlantsiAT1G14240.1; AT1G14240.1; AT1G14240. [Q9XI62-1]
AT1G14240.2; AT1G14240.2; AT1G14240. [Q9XI62-1]
AT1G14240.4; AT1G14240.4; AT1G14240. [Q9XI62-1]
GeneIDi837985.
KEGGiath:AT1G14240.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JF830008 mRNA. Translation: AEJ38084.1 .
AC007576 Genomic DNA. Translation: AAD39311.1 .
CP002684 Genomic DNA. Translation: AEE29127.1 .
CP002684 Genomic DNA. Translation: AEE29128.1 .
CP002684 Genomic DNA. Translation: AEE29130.1 .
BT006000 mRNA. Translation: AAO64935.1 .
AK227399 mRNA. Translation: BAE99403.1 .
AK317332 mRNA. Translation: BAH20006.1 .
PIRi D86276.
RefSeqi NP_001117284.1. NM_001123812.1. [Q9XI62-1 ]
NP_172876.1. NM_101290.4. [Q9XI62-1 ]
NP_973822.2. NM_202093.3. [Q9XI62-1 ]
UniGenei At.41982.

3D structure databases

ProteinModelPortali Q9XI62.
SMRi Q9XI62. Positions 68-460.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9XI62.
PRIDEi Q9XI62.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G14240.1 ; AT1G14240.1 ; AT1G14240 . [Q9XI62-1 ]
AT1G14240.2 ; AT1G14240.2 ; AT1G14240 . [Q9XI62-1 ]
AT1G14240.4 ; AT1G14240.4 ; AT1G14240 . [Q9XI62-1 ]
GeneIDi 837985.
KEGGi ath:AT1G14240.

Organism-specific databases

TAIRi AT1G14240.

Phylogenomic databases

eggNOGi COG5371.
HOGENOMi HOG000077482.
InParanoidi Q9XI62.
KOi K01510.
OMAi YLKTYCF.
PhylomeDBi Q9XI62.

Enzyme and pathway databases

BioCyci ARA:AT1G14240-MONOMER.
ARA:GQT-911-MONOMER.
ARA:GQT-912-MONOMER.

Gene expression databases

ExpressionAtlasi Q9XI62. baseline and differential.
Genevestigatori Q9XI62.

Family and domain databases

InterProi IPR000407. GDA1_CD39_NTPase.
[Graphical view ]
PANTHERi PTHR11782. PTHR11782. 1 hit.
Pfami PF01150. GDA1_CD39. 1 hit.
[Graphical view ]
PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional analyses of Arabidopsis apyrases 3 through 7."
    Yang J.
    Thesis (2011), University of Texas, United States
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
    Tissue: Root.

Entry informationi

Entry nameiAPY3_ARATH
AccessioniPrimary (citable) accession number: Q9XI62
Secondary accession number(s): Q3EDC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3