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Q9XI62

- APY3_ARATH

UniProt

Q9XI62 - APY3_ARATH

Protein

Probable apyrase 3

Gene

APY3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.By similarity

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

    Cofactori

    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei195 – 1951Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 8211ATP-bindingCuratedAdd
    BLAST
    Nucleotide bindingi219 – 22911ATP-bindingCuratedAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrolase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G14240-MONOMER.
    ARA:GQT-911-MONOMER.
    ARA:GQT-912-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable apyrase 3 (EC:3.6.1.5)
    Short name:
    AtAPY3
    Alternative name(s):
    ATP-diphosphatase
    ATP-diphosphohydrolase
    Adenosine diphosphatase
    Short name:
    ADPase
    NTPDase
    Nucleoside triphosphate diphosphohydrolase 3
    Gene namesi
    Name:APY3
    Ordered Locus Names:At1g14240
    ORF Names:F7A19.34
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G14240.

    Subcellular locationi

    Membrane By similarity; Single-pass type II membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 483483Probable apyrase 3PRO_0000420341Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9XI62.
    PRIDEiQ9XI62.

    Expressioni

    Tissue specificityi

    Expressed in the initiation zone of lateral root and in the lateral root tip, the adaxial junction of lateral shoots with the stems, and in the abscission zone of flower organs. Not expressed in the rosette leaves.1 Publication

    Gene expression databases

    ArrayExpressiQ9XI62.
    GenevestigatoriQ9XI62.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XI62.
    SMRiQ9XI62. Positions 68-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2929CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini51 – 483433ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei30 – 5021Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GDA1/CD39 NTPase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5371.
    HOGENOMiHOG000077482.
    InParanoidiQ9XI62.
    KOiK01510.
    OMAiYLKTYCF.
    PhylomeDBiQ9XI62.

    Family and domain databases

    InterProiIPR000407. GDA1_CD39_NTPase.
    [Graphical view]
    PANTHERiPTHR11782. PTHR11782. 1 hit.
    PfamiPF01150. GDA1_CD39. 1 hit.
    [Graphical view]
    PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9XI62-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTPETDALKV QILPKHQSLP YTVTKAKSKS LILLVVVSVT ITLGLLLYVF    50
    NSNSVISSGS LLSRRCKLRY SVLIDAGSSG TRVHVFGYWF ESGKPVFDFG 100
    EKHYANLKLT PGLSSYADNP EGASVSVTKL VEFAKQRIPK RMFRRSDIRL 150
    MATAGMRLLE VPVQEQILEV TRRVLRSSGF MFRDEWANVI SGSDEGIYSW 200
    ITANYALGSL GTDPLETTGI VELGGASAQV TFVSSEHVPP EYSRTIAYGN 250
    ISYTIYSHSF LDYGKDAALK KLLEKLQNSA NSTVDGVVED PCTPKGYIYD 300
    TNSKNYSSGF LADESKLKGS LQAAGNFSKC RSATFALLKE GKENCLYEHC 350
    SIGSTFTPDL QGSFLATASF YYTAKFFELE EKGWLSELIP AGKRYCGEEW 400
    SKLILEYPTT DEEYLRGYCF SAAYTISMLH DSLGIALDDE SITYASKAGE 450
    KHIPLDWALG AFILDVVTPN SDYNGKSRKY LGF 483
    Length:483
    Mass (Da):53,426
    Last modified:November 1, 1999 - v1
    Checksum:i4E31D13830F1C8F9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF830008 mRNA. Translation: AEJ38084.1.
    AC007576 Genomic DNA. Translation: AAD39311.1.
    CP002684 Genomic DNA. Translation: AEE29127.1.
    CP002684 Genomic DNA. Translation: AEE29128.1.
    CP002684 Genomic DNA. Translation: AEE29130.1.
    BT006000 mRNA. Translation: AAO64935.1.
    AK227399 mRNA. Translation: BAE99403.1.
    AK317332 mRNA. Translation: BAH20006.1.
    PIRiD86276.
    RefSeqiNP_001117284.1. NM_001123812.1. [Q9XI62-1]
    NP_172876.1. NM_101290.4. [Q9XI62-1]
    NP_973822.2. NM_202093.3. [Q9XI62-1]
    UniGeneiAt.41982.

    Genome annotation databases

    EnsemblPlantsiAT1G14240.1; AT1G14240.1; AT1G14240. [Q9XI62-1]
    AT1G14240.2; AT1G14240.2; AT1G14240. [Q9XI62-1]
    AT1G14240.4; AT1G14240.4; AT1G14240. [Q9XI62-1]
    GeneIDi837985.
    KEGGiath:AT1G14240.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF830008 mRNA. Translation: AEJ38084.1 .
    AC007576 Genomic DNA. Translation: AAD39311.1 .
    CP002684 Genomic DNA. Translation: AEE29127.1 .
    CP002684 Genomic DNA. Translation: AEE29128.1 .
    CP002684 Genomic DNA. Translation: AEE29130.1 .
    BT006000 mRNA. Translation: AAO64935.1 .
    AK227399 mRNA. Translation: BAE99403.1 .
    AK317332 mRNA. Translation: BAH20006.1 .
    PIRi D86276.
    RefSeqi NP_001117284.1. NM_001123812.1. [Q9XI62-1 ]
    NP_172876.1. NM_101290.4. [Q9XI62-1 ]
    NP_973822.2. NM_202093.3. [Q9XI62-1 ]
    UniGenei At.41982.

    3D structure databases

    ProteinModelPortali Q9XI62.
    SMRi Q9XI62. Positions 68-460.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9XI62.
    PRIDEi Q9XI62.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G14240.1 ; AT1G14240.1 ; AT1G14240 . [Q9XI62-1 ]
    AT1G14240.2 ; AT1G14240.2 ; AT1G14240 . [Q9XI62-1 ]
    AT1G14240.4 ; AT1G14240.4 ; AT1G14240 . [Q9XI62-1 ]
    GeneIDi 837985.
    KEGGi ath:AT1G14240.

    Organism-specific databases

    TAIRi AT1G14240.

    Phylogenomic databases

    eggNOGi COG5371.
    HOGENOMi HOG000077482.
    InParanoidi Q9XI62.
    KOi K01510.
    OMAi YLKTYCF.
    PhylomeDBi Q9XI62.

    Enzyme and pathway databases

    BioCyci ARA:AT1G14240-MONOMER.
    ARA:GQT-911-MONOMER.
    ARA:GQT-912-MONOMER.

    Gene expression databases

    ArrayExpressi Q9XI62.
    Genevestigatori Q9XI62.

    Family and domain databases

    InterProi IPR000407. GDA1_CD39_NTPase.
    [Graphical view ]
    PANTHERi PTHR11782. PTHR11782. 1 hit.
    Pfami PF01150. GDA1_CD39. 1 hit.
    [Graphical view ]
    PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional analyses of Arabidopsis apyrases 3 through 7."
      Yang J.
      Thesis (2011), University of Texas, United States
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
      Tissue: Root.

    Entry informationi

    Entry nameiAPY3_ARATH
    AccessioniPrimary (citable) accession number: Q9XI62
    Secondary accession number(s): Q3EDC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3