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Protein

Probable apyrase 3

Gene

APY3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.By similarity

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei195Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 82ATP-bindingCuratedAdd BLAST11
Nucleotide bindingi219 – 229ATP-bindingCuratedAdd BLAST11

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G14240-MONOMER.
ReactomeiR-ATH-8850843. Phosphate bond hydrolysis by NTPDase proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable apyrase 3 (EC:3.6.1.5)
Short name:
AtAPY3
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name:
ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 3
Gene namesi
Name:APY3
Ordered Locus Names:At1g14240
ORF Names:F7A19.34
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G14240.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 50Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini51 – 483ExtracellularSequence analysisAdd BLAST433

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004203411 – 483Probable apyrase 3Add BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Glycosylationi281N-linked (GlcNAc...)Sequence analysis1
Glycosylationi305N-linked (GlcNAc...)Sequence analysis1
Glycosylationi326N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9XI62.

Expressioni

Tissue specificityi

Expressed in the initiation zone of lateral root and in the lateral root tip, the adaxial junction of lateral shoots with the stems, and in the abscission zone of flower organs. Not expressed in the rosette leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ9XI62. baseline and differential.
GenevisibleiQ9XI62. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G14240.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XI62.
SMRiQ9XI62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1386. Eukaryota.
COG5371. LUCA.
HOGENOMiHOG000077482.
InParanoidiQ9XI62.
KOiK01510.
OMAiCRSATFA.
OrthoDBiEOG09360BC0.
PhylomeDBiQ9XI62.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9XI62-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTPETDALKV QILPKHQSLP YTVTKAKSKS LILLVVVSVT ITLGLLLYVF
60 70 80 90 100
NSNSVISSGS LLSRRCKLRY SVLIDAGSSG TRVHVFGYWF ESGKPVFDFG
110 120 130 140 150
EKHYANLKLT PGLSSYADNP EGASVSVTKL VEFAKQRIPK RMFRRSDIRL
160 170 180 190 200
MATAGMRLLE VPVQEQILEV TRRVLRSSGF MFRDEWANVI SGSDEGIYSW
210 220 230 240 250
ITANYALGSL GTDPLETTGI VELGGASAQV TFVSSEHVPP EYSRTIAYGN
260 270 280 290 300
ISYTIYSHSF LDYGKDAALK KLLEKLQNSA NSTVDGVVED PCTPKGYIYD
310 320 330 340 350
TNSKNYSSGF LADESKLKGS LQAAGNFSKC RSATFALLKE GKENCLYEHC
360 370 380 390 400
SIGSTFTPDL QGSFLATASF YYTAKFFELE EKGWLSELIP AGKRYCGEEW
410 420 430 440 450
SKLILEYPTT DEEYLRGYCF SAAYTISMLH DSLGIALDDE SITYASKAGE
460 470 480
KHIPLDWALG AFILDVVTPN SDYNGKSRKY LGF
Length:483
Mass (Da):53,426
Last modified:November 1, 1999 - v1
Checksum:i4E31D13830F1C8F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF830008 mRNA. Translation: AEJ38084.1.
AC007576 Genomic DNA. Translation: AAD39311.1.
CP002684 Genomic DNA. Translation: AEE29127.1.
CP002684 Genomic DNA. Translation: AEE29128.1.
CP002684 Genomic DNA. Translation: AEE29130.1.
BT006000 mRNA. Translation: AAO64935.1.
AK227399 mRNA. Translation: BAE99403.1.
AK317332 mRNA. Translation: BAH20006.1.
PIRiD86276.
RefSeqiNP_001117284.1. NM_001123812.2. [Q9XI62-1]
NP_172876.1. NM_101290.5. [Q9XI62-1]
NP_973822.2. NM_202093.4. [Q9XI62-1]
UniGeneiAt.41982.

Genome annotation databases

EnsemblPlantsiAT1G14240.1; AT1G14240.1; AT1G14240. [Q9XI62-1]
AT1G14240.2; AT1G14240.2; AT1G14240. [Q9XI62-1]
AT1G14240.4; AT1G14240.4; AT1G14240. [Q9XI62-1]
GeneIDi837985.
GrameneiAT1G14240.1; AT1G14240.1; AT1G14240.
AT1G14240.2; AT1G14240.2; AT1G14240.
AT1G14240.4; AT1G14240.4; AT1G14240.
KEGGiath:AT1G14240.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF830008 mRNA. Translation: AEJ38084.1.
AC007576 Genomic DNA. Translation: AAD39311.1.
CP002684 Genomic DNA. Translation: AEE29127.1.
CP002684 Genomic DNA. Translation: AEE29128.1.
CP002684 Genomic DNA. Translation: AEE29130.1.
BT006000 mRNA. Translation: AAO64935.1.
AK227399 mRNA. Translation: BAE99403.1.
AK317332 mRNA. Translation: BAH20006.1.
PIRiD86276.
RefSeqiNP_001117284.1. NM_001123812.2. [Q9XI62-1]
NP_172876.1. NM_101290.5. [Q9XI62-1]
NP_973822.2. NM_202093.4. [Q9XI62-1]
UniGeneiAt.41982.

3D structure databases

ProteinModelPortaliQ9XI62.
SMRiQ9XI62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G14240.1.

Proteomic databases

PaxDbiQ9XI62.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G14240.1; AT1G14240.1; AT1G14240. [Q9XI62-1]
AT1G14240.2; AT1G14240.2; AT1G14240. [Q9XI62-1]
AT1G14240.4; AT1G14240.4; AT1G14240. [Q9XI62-1]
GeneIDi837985.
GrameneiAT1G14240.1; AT1G14240.1; AT1G14240.
AT1G14240.2; AT1G14240.2; AT1G14240.
AT1G14240.4; AT1G14240.4; AT1G14240.
KEGGiath:AT1G14240.

Organism-specific databases

TAIRiAT1G14240.

Phylogenomic databases

eggNOGiKOG1386. Eukaryota.
COG5371. LUCA.
HOGENOMiHOG000077482.
InParanoidiQ9XI62.
KOiK01510.
OMAiCRSATFA.
OrthoDBiEOG09360BC0.
PhylomeDBiQ9XI62.

Enzyme and pathway databases

BioCyciARA:AT1G14240-MONOMER.
ReactomeiR-ATH-8850843. Phosphate bond hydrolysis by NTPDase proteins.

Miscellaneous databases

PROiQ9XI62.

Gene expression databases

ExpressionAtlasiQ9XI62. baseline and differential.
GenevisibleiQ9XI62. AT.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPY3_ARATH
AccessioniPrimary (citable) accession number: Q9XI62
Secondary accession number(s): Q3EDC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.