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Q9XI62 (APY3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable apyrase 3

Short name=AtAPY3
EC=3.6.1.5
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name=ADPase
NTPDase
Nucleoside triphosphate diphosphohydrolase 3
Gene names
Name:APY3
Ordered Locus Names:At1g14240
ORF Names:F7A19.34
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates By similarity.

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor

Calcium By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Expressed in the initiation zone of lateral root and in the lateral root tip, the adaxial junction of lateral shoots with the stems, and in the abscission zone of flower organs. Not expressed in the rosette leaves. Ref.1

Disruption phenotype

No visible phenotype. Ref.1

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandATP-binding
Calcium
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9XI62-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 483483Probable apyrase 3
PRO_0000420341

Regions

Topological domain1 – 2929Cytoplasmic Potential
Transmembrane30 – 5021Helical; Signal-anchor for type II membrane protein; Potential
Topological domain51 – 483433Extracellular Potential
Nucleotide binding72 – 8211ATP-binding Probable
Nucleotide binding219 – 22911ATP-binding Probable

Sites

Active site1951Proton acceptor By similarity

Amino acid modifications

Glycosylation2501N-linked (GlcNAc...) Potential
Glycosylation2811N-linked (GlcNAc...) Potential
Glycosylation3051N-linked (GlcNAc...) Potential
Glycosylation3261N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 4E31D13830F1C8F9

FASTA48353,426
        10         20         30         40         50         60 
MTPETDALKV QILPKHQSLP YTVTKAKSKS LILLVVVSVT ITLGLLLYVF NSNSVISSGS 

        70         80         90        100        110        120 
LLSRRCKLRY SVLIDAGSSG TRVHVFGYWF ESGKPVFDFG EKHYANLKLT PGLSSYADNP 

       130        140        150        160        170        180 
EGASVSVTKL VEFAKQRIPK RMFRRSDIRL MATAGMRLLE VPVQEQILEV TRRVLRSSGF 

       190        200        210        220        230        240 
MFRDEWANVI SGSDEGIYSW ITANYALGSL GTDPLETTGI VELGGASAQV TFVSSEHVPP 

       250        260        270        280        290        300 
EYSRTIAYGN ISYTIYSHSF LDYGKDAALK KLLEKLQNSA NSTVDGVVED PCTPKGYIYD 

       310        320        330        340        350        360 
TNSKNYSSGF LADESKLKGS LQAAGNFSKC RSATFALLKE GKENCLYEHC SIGSTFTPDL 

       370        380        390        400        410        420 
QGSFLATASF YYTAKFFELE EKGWLSELIP AGKRYCGEEW SKLILEYPTT DEEYLRGYCF 

       430        440        450        460        470        480 
SAAYTISMLH DSLGIALDDE SITYASKAGE KHIPLDWALG AFILDVVTPN SDYNGKSRKY 


LGF 

« Hide

References

« Hide 'large scale' references
[1]"Functional analyses of Arabidopsis apyrases 3 through 7."
Yang J.
Thesis (2011), University of Texas, United States
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
Tissue: Root.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JF830008 mRNA. Translation: AEJ38084.1.
AC007576 Genomic DNA. Translation: AAD39311.1.
CP002684 Genomic DNA. Translation: AEE29127.1.
CP002684 Genomic DNA. Translation: AEE29128.1.
CP002684 Genomic DNA. Translation: AEE29130.1.
BT006000 mRNA. Translation: AAO64935.1.
AK227399 mRNA. Translation: BAE99403.1.
AK317332 mRNA. Translation: BAH20006.1.
PIRD86276.
RefSeqNP_001117284.1. NM_001123812.1. [Q9XI62-1]
NP_172876.1. NM_101290.4. [Q9XI62-1]
NP_973822.2. NM_202093.3. [Q9XI62-1]
UniGeneAt.41982.

3D structure databases

ProteinModelPortalQ9XI62.
SMRQ9XI62. Positions 68-460.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9XI62.
PRIDEQ9XI62.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G14240.1; AT1G14240.1; AT1G14240. [Q9XI62-1]
AT1G14240.2; AT1G14240.2; AT1G14240. [Q9XI62-1]
AT1G14240.4; AT1G14240.4; AT1G14240. [Q9XI62-1]
GeneID837985.
KEGGath:AT1G14240.

Organism-specific databases

TAIRAT1G14240.

Phylogenomic databases

eggNOGCOG5371.
HOGENOMHOG000077482.
InParanoidQ9XI62.
KOK01510.
OMAYLKTYCF.
PhylomeDBQ9XI62.

Enzyme and pathway databases

BioCycARA:AT1G14240-MONOMER.
ARA:GQT-911-MONOMER.
ARA:GQT-912-MONOMER.

Gene expression databases

ArrayExpressQ9XI62.
GenevestigatorQ9XI62.

Family and domain databases

InterProIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERPTHR11782. PTHR11782. 1 hit.
PfamPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPY3_ARATH
AccessionPrimary (citable) accession number: Q9XI62
Secondary accession number(s): Q3EDC8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: November 1, 1999
Last modified: May 14, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names