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Protein

Proteasome subunit beta type-3-A

Gene

PBC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-382556. ABC-family proteins mediated transport.
R-ATH-4641258. Degradation of DVL.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5668541. TNFR2 non-canonical NF-kB pathway.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-3-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit C-1
Proteasome component T
Gene namesi
Name:PBC1
Synonyms:PRCT
Ordered Locus Names:At1g21720
ORF Names:F8K7.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G21720.
TAIRilocus:2036972. AT1G21720.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001480652 – 204Proteasome subunit beta type-3-AAdd BLAST203

Proteomic databases

PaxDbiQ9XI05.
PRIDEiQ9XI05.

Expressioni

Gene expression databases

ExpressionAtlasiQ9XI05. baseline and differential.
GenevisibleiQ9XI05. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi24015. 2 interactors.
STRINGi3702.AT1G21720.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XI05.
SMRiQ9XI05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0180. Eukaryota.
ENOG410XSC8. LUCA.
HOGENOMiHOG000090523.
InParanoidiQ9XI05.
KOiK02735.
OMAiEQLFGMC.
OrthoDBiEOG09360M56.
PhylomeDBiQ9XI05.

Family and domain databases

CDDicd03759. proteasome_beta_type_3. 1 hit.
Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR033811. Proteasome_beta_3.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9XI05-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSIFEYNGSA VVAMVGKNCF AIASDRRLGV QLQTIATDFQ RISKIHDRVF
60 70 80 90 100
IGLSGLATDV QTLYQRLVFR HKLYQLREER DMKPETFASL VSAILYEKRF
110 120 130 140 150
GPYLCQPVIA GLGDDDKPFI CTMDSIGAKE LAKDFVVSGT ASESLYGACE
160 170 180 190 200
AMYKPDMEAE ELFETISQAL LSSVDRDCLS GWGGHVYIVT PTEIKERILK

GRMD
Length:204
Mass (Da):22,798
Last modified:February 9, 2010 - v2
Checksum:iB52E22CDB3EDA467
GO
Isoform 2 (identifier: Q9XI05-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-2: S → Q

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:204
Mass (Da):22,839
Checksum:iE1CD9513D8D2F3B9
GO

Sequence cautioni

The sequence AAC32068 differs from that shown. Reason: Frameshift at position 41.Curated
The sequence CAA73616 differs from that shown. Reason: Frameshift at position 41.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8G → V in AAM62756 (Ref. 7) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0161432S → Q in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13173 mRNA. Translation: CAA73616.1. Frameshift.
AF043532 mRNA. Translation: AAC32068.1. Frameshift.
AC007727 Genomic DNA. Translation: AAD41426.1.
CP002684 Genomic DNA. Translation: AEE30146.1.
AY065070 mRNA. Translation: AAL38246.1.
AY114628 mRNA. Translation: AAM47947.1.
AF344327 mRNA. Translation: AAK06878.1.
AK226397 mRNA. Translation: BAE98543.1.
AY085532 mRNA. Translation: AAM62756.1.
PIRiF86350.
RefSeqiNP_564149.1. NM_102021.3. [Q9XI05-1]
UniGeneiAt.11467.

Genome annotation databases

EnsemblPlantsiAT1G21720.1; AT1G21720.1; AT1G21720. [Q9XI05-1]
GeneIDi838776.
GrameneiAT1G21720.1; AT1G21720.1; AT1G21720.
KEGGiath:AT1G21720.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSB3A_ARATH
AccessioniPrimary (citable) accession number: Q9XI05
Secondary accession number(s): O23707
, Q0WWF5, Q8LEA7, Q8VZC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: February 9, 2010
Last modified: October 25, 2017
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families