Q9XI00 (SKI32_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein 7

Alternative name(s):
SKP1-interacting partner 32

Gene names

Name:	SKIP32
Synonyms:	FBP7
Ordered Locus Names:	At1g21760
ORF Names:	F8K7.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity. Required for efficient protein synthesis during temperature stress conditions. Ref.7
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex. Interacts with SKP1A/ASK1, SKP1B/ASK2, ASK4, ASK11 and ASK14. Ref.5 Ref.6 Ref.7
Subcellular location	Nucleus. Mitochondrion Ref.7.
Tissue specificity	Expressed in leaves, flowers and stems, and, to a lower extent, in roots and siliques. Ref.7
Developmental stage	In seedlings, mostly localized in shoot meristems, root tips and hypocotyl. In adult plant, strongest levels observed in the vasculature, pollen, pollen tubes, and trichomes. Also found in senescencing leaves. Ref.7
Induction	By temperature stresses. Ref.7
Domain	The F-box is necessary for the interaction with ASK proteins By similarity. Ref.7
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Stress response Ubl conjugation pathway
Cellular component	Mitochondrion Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of translation Inferred from mutant phenotype Ref.7. Source: TAIR response to cold Inferred from mutant phenotype Ref.7. Source: TAIR response to heat Inferred from mutant phenotype Ref.7. Source: TAIR
Cellular component	mitochondrion Inferred from direct assay Ref.7. Source: TAIR nucleus Inferred from direct assay Ref.7. Source: TAIR pollen tube Inferred from direct assay Ref.7. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	F-box protein 7		PRO_0000272205
Regions
	Domain	56 – 102	47	F-box
	Region	126 – 158	33	Mystery domain 1 (MD1)
	Region	163 – 201	39	Mystery domain 2 (MD2)

Sequences

	Sequence		Length	Mass (Da)
	Q9XI00 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 4A6CD30291D78908	FASTA	328	38,043
	10 20 30 40 50 60 MTSDALTIPS ELESALRLRT VQYFITKRPW LDLYGVHVRP VPPFGSTSRK PHFDPALIHR 70 80 90 100 110 120 CLPDELLFEV FARMMPYDLG RASCVCRKWR YTVRNPMFWR NACLKAWQTA GVIENYKILQ 130 140 150 160 170 180 SKYDGSWRKM WLLRSRVRTD GLYVSRNTYI RAGIAEWKIT NPVHIVCYYR YIRFYPSGRF 190 200 210 220 230 240 LYKNSSQKLK DVAKYMNFKA SKSENLYKGT YTLSMSDDKI EAAVLYPGTR PTVLRIRLRL 250 260 270 280 290 300 RGTAIGANNR MDLLSLVTSG VNDEEISSTE EDILGLVEGW EDDETHNPDI PAVSHKRGMT 310 320 AFVFVPFEEV DESVLNLPPE KMDYYVTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis." Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D. Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002) [PubMed: 12169662] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK4; ASK11 AND ASK14.
[6]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKPIA/ASK1.
[7]	"The evolutionarily conserved Arabidopsis thaliana F-box protein AtFBP7 is required for efficient translation during temperature stress." Calderon-Villalobos L.I.A., Nill C., Marrocco K., Kretsch T., Schwechheimer C. Gene 392:106-116(2007) [PubMed: 17240087] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INDUCTION, DOMAINS MD1 AND MD2, INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK4; ASK11 AND ASK14.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007727 Genomic DNA. Translation: AAD41431.1. CP002684 Genomic DNA. Translation: AEE30153.1. BT006427 mRNA. Translation: AAP21235.1. AK228033 mRNA. Translation: BAE99994.1.
IPI	IPI00518971.
PIR	C86351.
RefSeq	NP_564150.1. NM_102025.3.
UniGene	At.43381. At.71064. At.71563.
3D structure databases
ProteinModelPortal	Q9XI00.
SMR	Q9XI00. Positions 59-139.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9XI00. 4 interactions.
STRING	Q9XI00.
Proteomic databases
PRIDE	Q9XI00.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G21760.1; AT1G21760.1; AT1G21760.
GeneID	838780.
GenomeReviews	Gene locus AT1G21760 in contig CT485782_GR.
KEGG	ath:AT1G21760.
NMPDR	fig|3702.1.peg.2537.
Organism-specific databases
TAIR	At1g21760.
Phylogenomic databases
eggNOG	KOG2997.
GeneTree	EPGT00050000019717.
HOGENOM	HBG609138.
InParanoid	Q9XI00.
OMA	FVFVPFE.
PhylomeDB	Q9XI00.
ProtClustDB	CLSN2687901.
Gene expression databases
Genevestigator	Q9XI00.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. IPR022364. F-box_dom_Skp2-like. [Graphical view]
KO	K10295.
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SKI32_ARATH

Accession

Primary (citable) accession number: Q9XI00

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	November 1, 1999
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program