Reviewed, UniProtKB/Swiss-Prot Q9XHX0 (EXPA8_ORYSJ)

Names and origin

Protein names

Recommended name:
    Expansin-A8
Alternative name(s):
    OsEXPA8
    Alpha-expansin-8
    OsEXP8
    OsaEXPa1.17

Gene names

Name:	EXPA8
Synonyms:	EXP8
Ordered Locus Names:	Os01g0248900, LOC_Os01g14650
ORF Names:	OSJNBa0049B20.23, P0034C11.30

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found. May be required for rapid internodal elongation in deepwater rice during submergence By similarity.
Subcellular location	Secreted › cell wall By similarity. Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in roots. Ref.6
Sequence similarities	Belongs to the expansin family. Expansin A subfamily. Contains 1 expansin-like CBD domain. Contains 1 expansin-like EG45 domain.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Membrane Secreted
Domain	Signal
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	plant-type cell wall organization Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 251	229	Expansin-A8		PRO_0000251987
Regions
	Domain	43 – 157	115	Expansin-like EG45
	Domain	167 – 246	80	Expansin-like CBD
Amino acid modifications
	Disulfide bond	46 ↔ 74		By similarity
	Disulfide bond	77 ↔ 152		By similarity
	Disulfide bond	82 ↔ 90		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XHX0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 1A0BB36709FDF9A3	FASTA	251	26,334
	10 20 30 40 50 60 MAAARMLVLL ASLCALLLTA SAAKWTPAFA TFYGGSDASG TMGGACGYGD LYGAGYGTRT 70 80 90 100 110 120 AALSTALFNG GASCGACFTI ACDTRKTQWC KPGTSITVTA TNFCPPNYAL SGDAGGWCNP 130 140 150 160 170 180 PRRHFDMSQP AWETIAVYRA GIVPVNYRRV PCQRSGGIRF AVNGHSYFEL VLVTNVGGSG 190 200 210 220 230 240 AVAQMWIKGS GTGWMAMSRN WGANWQSNAR LDGQALSFRV QADDGRVVTA ADVAPAGWSF 250 GATYTSSAQF Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Nomenclature for members of the expansin superfamily of genes and proteins." Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J., Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C., Voesenek L.A.C. Plant Mol. Biol. 55:311-314(2004) [PubMed: 15604683] [Abstract] Cited for: NOMENCLATURE.
[6]	"Characterization and transcriptional expression of the alpha-expansin gene family in rice." Shin J.-H., Jeong D.-H., Park M.C., An G. Mol. Cells 20:210-218(2005) [PubMed: 16267395] [Abstract] Cited for: TISSUE SPECIFICITY.

Web resources

EXPANSIN homepage

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007789 Genomic DNA. Translation: AAD38296.1. AP002865 Genomic DNA. Translation: BAB18336.1. AP008207 Genomic DNA. Translation: BAF04501.1. AK111100 mRNA. No translation available.
RefSeq	NP_001042587.1.
UniGene	Os.28773
3D structure databases
HSSP	HSSP built from PDB template 1N10 based on UniProtKB P43213.
SMR	Q9XHX0. Positions 22-251.
ModBase	Search...
Genome annotation databases
GeneID	4327217.
KEGG	osa:4327217.
NMPDR	fig|39947.1.peg.5746.
Organism-specific databases
Gramene	Q9XHX0.
Phylogenomic databases
HOGENOM	HBG605129.
Family and domain databases
InterPro	IPR009009. Barwin-related_endoglucanase. IPR007112. Expan_endogl. IPR007118. Expan_Lol_pI. IPR007117. Expan_Lol_pI_C. IPR002963. Expansin. IPR014734. Pollen_allergen. IPR005132. RlpA. [Graphical view]
Gene3D	G3DSA:2.60.40.760. Expan_Lol_pI_C. 1 hit. G3DSA:2.40.40.40. Pollen_allergen. 1 hit.
Pfam	PF03330. DPBB_1. 1 hit. PF01357. Pollen_allerg_1. 1 hit. [Graphical view]
PRINTS	PR01226. EXPANSIN. PR01225. EXPANSNFAMLY.
PROSITE	PS50843. EXPANSIN_CBD. 1 hit. PS50842. EXPANSIN_EG45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXPA8_ORYSJ

Accession

Primary (citable) accession number: Q9XHX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)