Reviewed, UniProtKB/Swiss-Prot Q9XHM5 (G2OX2_PEA)

Names and origin

Protein names

Recommended name:
    Gibberellin 2-beta-dioxygenase 2
    EC=1.14.11.13
Alternative name(s):
    Gibberellin 2-beta-hydroxylase 2
    Gibberellin 2-oxidase 2
    GA 2-oxidase 2

Gene names

Name:

GA2OX2

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8. Ref.1
Catalytic activity	Gibberellin 1 + 2-oxoglutarate + O2 = 2-beta-hydroxygibberellin 1 + succinate + CO2.
Cofactor	Iron By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Tissue specificity	Predominantly expressed in leaves. Ref.1
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gibberellin 2-beta-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Gibberellin 2-beta-dioxygenase 2		PRO_0000067312
Sites
	Active site	276	1	Potential
	Metal binding	209	1	Iron By similarity
	Metal binding	211	1	Iron By similarity
	Metal binding	266	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XHM5-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 64F4097DF54A64DD	FASTA	345	38,679
	10 20 30 40 50 60 MVVPSPTSMI RTKKTKAVGI PTIDLSLERS QLSELVVKAC EEYGFFKVVN HSVPKEVISR 70 80 90 100 110 120 LDEEGIEFFS KNSSEKRQAG TSTPFGYGCK NIGPNGDKGE LEYLLLHSNP ISISERSKTI 130 140 150 160 170 180 AKDHPIKFSC IVNDYIKAVK DLTCEILELA AEGLWVPDKS SLSKIIKDEH SDSLLRINHY 190 200 210 220 230 240 PPVKKLGNDN WDPSKIQNSN NNNIGFGEHS DPQILTILRS NNVGGLQIST HHGLWIPVPP 250 260 270 280 290 300 DPSEFYVMVG DALQVLTNGR FVSVRHRVLT NTTKPRMSMM YFAAPPLNWL ISPLSKMVTA 310 320 330 340 HSPCLYRPFT WAQYKQAAYA LRLGDTRLDQ FKVQKQEDSN DSHSL

References

[1]	"Gibberellin 2-oxidation and the SLN gene of Pisum sativum." Lester D.R., Ross J.J., Smith J.J., Elliott R.C., Reid J.B. Plant J. 19:65-73(1999) [PubMed: 10417727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: cv. Torsdag. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF100954 mRNA. Translation: AAD45424.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11640.
BRENDA	1.14.11.13. 287.
Family and domain databases
InterPro	IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
ProtoNet	Search...

Entry information

Entry name

G2OX2_PEA

Accession

Primary (citable) accession number: Q9XHM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	November 1, 1999
Last modified:	September 22, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)