Reviewed, UniProtKB/Swiss-Prot Q9XHL5 (HMDH3_ORYSJ)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 3
      Short name=HMG-CoA reductase 3
    EC=1.1.1.34

Gene names

Name:	HMG3
Synonyms:	HMGR3
Ordered Locus Names:	Os08g0512700, LOC_Os08g40180
ORF Names:	P0711H09.15

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	3-hydroxy-3-methylglutaryl-coenzyme A reductase 3		PRO_0000114447
Regions
	Transmembrane	25 – 45	21	Potential
	Transmembrane	69 – 89	21	Potential
	Region	90 – 145	56	Linker By similarity
	Region	146 – 561	416	Catalytic By similarity
Sites
	Active site	240	1	Charge relay system By similarity
	Active site	372	1	Charge relay system By similarity
	Active site	448	1	Charge relay system By similarity
	Active site	546	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Glycosylation	550	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	119 – 121	3	PPP → RA in AAD38873. Ref.1
	Sequence conflict	345	1	V → E in AAD38873. Ref.1
	Sequence conflict	538	1	A → R in AAD38873. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9XHL5-1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: B2A826D6A1BA0B1C	FASTA	561	59,401
	10 20 30 40 50 60 MEVRRRAPLP PPPGRVQAGD ALPLPIRHTN LIFSALFAAS LAYLMRRWRE KIRSSTPLHV 70 80 90 100 110 120 VGLAEMLAIF GLVASLIYLL SFFGIAFVQS IVSSSDDEEE DFLVGPARGS SAAAAVAPPP 130 140 150 160 170 180 PPSSPAQCSL LGSPHDDAAR ERMPEEDEEI VSSVVAGKVP SYVLETKLGD CRRAAGIRRE 190 200 210 220 230 240 AVRRITGRQI EGLPLDGFDY ASILGQCCEL PVGYVQLPVG IAGPLLLDGQ RFYVPMATTE 250 260 270 280 290 300 GCLVASTNRG CKAIAESGGA VSVVLRDGMT RAPVARLPTA RRAAELKAFL EDSVNFNTLS 310 320 330 340 350 360 MVFNRSSRFA RLQGVQCAMA GRNLYMRFSC CTGDAMGMNM VSKGVQNVLD YLQDDFPDMD 370 380 390 400 410 420 VISISGNFCS DKKPAAVNWI EGRGKSVVCE AVIKEDVVKK VLKTNVQSLV ELNVIKNLAG 430 440 450 460 470 480 SAVAGALGGF NAHASNIVTA IFIATGQDPA QNVESSHCIT MLEAVNDGRD LHISVTMPSI 490 500 510 520 530 540 EVGTVGGGTQ LASQAACLDL LGVKGANRES PGSNARLLAT VVAGGVLAGE LSLLSALAAG 550 560 QLVKSHMKYN RSSKDMSKVI S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of two differentially expressed hydroxy-methylglutaryl-CoA reductase genes from rice." Ha S.-H., Lee S.-W., Hwang Y.-S. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Oryza sativa nipponbare chromosome 8 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 95-561. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF110382 Genomic DNA. Translation: AAD38873.1. AP004765 Genomic DNA. Translation: BAD10066.1. AK060545 mRNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1DQA based on UniProtKB P04035.
ModBase	Search...
Organism-specific databases
Gramene	Q9XHL5.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH3_ORYSJ

Accession

Primary (citable) accession number: Q9XHL5
Secondary accession number(s): Q6Z8N5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 2, 2006
Last modified:	November 25, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)