Reviewed, UniProtKB/Swiss-Prot Q9XHG2 (FLS_MALDO)

Names and origin

Protein names

Recommended name:
    Flavonol synthase/flavanone 3-hydroxylase
      Short name=FLS
    EC=1.14.11.23
    EC=1.14.11.9

Gene names

Name:

FLS

Organism

Malus domestica (Apple) (Pyrus malus)

Taxonomic identifier

3750 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Maloideae › Malus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin By similarity.
Catalytic activity	A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O. A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 ascorbate molecule per subunit By similarity.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW flavonol synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Flavonol synthase/flavanone 3-hydroxylase		PRO_0000067294
Regions
	Domain	198 – 298	101	Fe2OG dioxygenase
Sites
	Metal binding	223	1	Iron By similarity
	Metal binding	225	1	Iron By similarity
	Metal binding	279	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XHG2-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 49AE7236145A6072	FASTA	337	38,123
	10 20 30 40 50 60 MGVESVERER ESNEGTIPAE FIRSENEQPG ITTVHGKVLE VPIIDFSDPD EEKLIVQITE 70 80 90 100 110 120 ASSNWGMYQI VNHDIPSEVI SKLQAVGKEF FELPQEEKEA YAKPPDSASI EGYGTKLFKE 130 140 150 160 170 180 ISEGDTTKKG WVDNLFNKIW PPSVVNYQFW PKNPPSYREA NEEYAKHLHN VVEKLFRLLS 190 200 210 220 230 240 LGLGLEGQEL KKAAGGDNLE YLLKINYYPP CPRPDLALGV VAHTDMSTVT ILVPNDVQGL 250 260 270 280 290 300 QACKDGRWYD VKYIPNALVI HIGDQMEIMS NGKYTSVLHR TTVNKDKTRI SWPVFLEPPA 310 320 330 DHVVGPHPQL VNAVNQPKYK TKKYGDYVYC KINKLPQ

References

[1]	"Molecular cloning and expression of a flavonol synthase cDNA from 'Fuji' apple." Lee J.-R., Hong S.-T., Kim S.-R. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Fuji. Tissue: Peelings.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF119095 mRNA. Translation: AAD26261.1.
3D structure databases
SMR	Q9XHG2. Positions 1-334.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.11.23. 66979. 1.14.11.9. 66979.
Family and domain databases
InterPro	IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLS_MALDO

Accession

Primary (citable) accession number: Q9XHG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)