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Q9XHE8

- C71DI_MENSP

UniProt

Q9XHE8 - C71DI_MENSP

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Protein

Cytochrome P450 71D18

Gene

CYP71D18

Organism
Mentha spicata (Spearmint)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydroxylates (-)-(4S)-limonene to (-)-trans-carveol, a precursor of (-)-carvone, responsible for the typical spearmint flavor note. Fluorinated substrate analogs are hydroxylated with the same regio- and stereochemistry.2 Publications

Catalytic activityi

--(S)-limonene + NADPH + O2 = (-)-trans-carveol + NADP+ + H2O.2 Publications

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. (S)-limonene 6-monooxygenase activity Source: UniProtKB
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15424.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 71D18 (EC:1.14.13.48)
Alternative name(s):
(-)-(4S)-Limonene-6-hydroxylase
Gene namesi
Name:CYP71D18
OrganismiMentha spicata (Spearmint)
Taxonomic identifieri29719 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi357 – 3571L → M: No effect on regiospecificity. 1 Publication
Mutagenesisi359 – 3591L → M: No effect on regiospecificity. 1 Publication
Mutagenesisi363 – 3631F → I: Conversion to the regiospecificity and catalytic efficiency of the peppermint limonene-3-hydroxylase. 1 Publication
Mutagenesisi369 – 3691Q → S: No effect on regiospecificity. 1 Publication
Mutagenesisi370 – 3701S → C: No effect on regiospecificity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Cytochrome P450 71D18PRO_0000389497Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9XHE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XHE8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELDLLSAII ILVATYIVSL LINQWRKSKS QQNLPPSPPK LPVIGHLHFL
60 70 80 90 100
WGGLPQHVFR SIAQKYGPVA HVQLGEVYSV VLSSAEAAKQ AMKVLDPNFA
110 120 130 140 150
DRFDGIGSRT MWYDKDDIIF SPYNDHWRQM RRICVTELLS PKNVRSFGYI
160 170 180 190 200
RQEEIERLIR LLGSSGGAPV DVTEEVSKMS CVVVCRAAFG SVLKDQGSLA
210 220 230 240 250
ELVKESLALA SGFELADLYP SSWLLNLLSL NKYRLQRMRR RLDHILDGFL
260 270 280 290 300
EEHREKKSGE FGGEDIVDVL FRMQKGSDIK IPITSNCIKG FIFDTFSAGA
310 320 330 340 350
ETSSTTISWA LSELMRNPAK MAKVQAEVRE ALKGKTVVDL SEVQELKYLR
360 370 380 390 400
SVLKETLRLH PPFPLIPRQS REECEVNGYT IPAKTRIFIN VWAIGRDPQY
410 420 430 440 450
WEDPDTFRPE RFDEVSRDFM GNDFEFIPFG AGRRICPGLH FGLANVEIPL
460 470 480 490
AQLLYHFDWK LPQGMTDADL DMTETPGLSG PKKKNVCLVP TLYKSP
Length:496
Mass (Da):56,149
Last modified:November 1, 1999 - v1
Checksum:i06E4370F95EB91C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124815 mRNA. Translation: AAD44150.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Mint condition - Issue 113 of January 2010

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124815 mRNA. Translation: AAD44150.1 .

3D structure databases

ProteinModelPortali Q9XHE8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-15424.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
    Lupien S., Karp F., Wildung M., Croteau R.
    Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-21; 176-200 AND 375-398, CATALYTIC ACTIVITY, FUNCTION.
  2. "A single amino acid substitution (F363I) converts the regiochemistry of the spearmint (-)-limonene hydroxylase from a C6- to a C3-hydroxylase."
    Schalk M., Croteau R.
    Proc. Natl. Acad. Sci. U.S.A. 97:11948-11953(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LEU-357; LEU-359; PHE-363; GLN-369 AND SER-370.
  3. "Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
    Wuest M., Little D.B., Schalk M., Croteau R.
    Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiC71DI_MENSP
AccessioniPrimary (citable) accession number: Q9XHE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3