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Q9XHE8

- C71DI_MENSP

UniProt

Q9XHE8 - C71DI_MENSP

Protein

Cytochrome P450 71D18

Gene

CYP71D18

Organism
Mentha spicata (Spearmint)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Hydroxylates (-)-(4S)-limonene to (-)-trans-carveol, a precursor of (-)-carvone, responsible for the typical spearmint flavor note. Fluorinated substrate analogs are hydroxylated with the same regio- and stereochemistry.2 Publications

    Catalytic activityi

    --(S)-limonene + NADPH + O2 = (-)-trans-carveol + NADP+ + H2O.2 Publications

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi436 – 4361Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. (S)-limonene 6-monooxygenase activity Source: UniProtKB
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15424.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 71D18 (EC:1.14.13.48)
    Alternative name(s):
    (-)-(4S)-Limonene-6-hydroxylase
    Gene namesi
    Name:CYP71D18
    OrganismiMentha spicata (Spearmint)
    Taxonomic identifieri29719 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. intracellular membrane-bounded organelle Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi357 – 3571L → M: No effect on regiospecificity. 1 Publication
    Mutagenesisi359 – 3591L → M: No effect on regiospecificity. 1 Publication
    Mutagenesisi363 – 3631F → I: Conversion to the regiospecificity and catalytic efficiency of the peppermint limonene-3-hydroxylase. 1 Publication
    Mutagenesisi369 – 3691Q → S: No effect on regiospecificity. 1 Publication
    Mutagenesisi370 – 3701S → C: No effect on regiospecificity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496Cytochrome P450 71D18PRO_0000389497Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XHE8.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2 – 2221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XHE8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELDLLSAII ILVATYIVSL LINQWRKSKS QQNLPPSPPK LPVIGHLHFL    50
    WGGLPQHVFR SIAQKYGPVA HVQLGEVYSV VLSSAEAAKQ AMKVLDPNFA 100
    DRFDGIGSRT MWYDKDDIIF SPYNDHWRQM RRICVTELLS PKNVRSFGYI 150
    RQEEIERLIR LLGSSGGAPV DVTEEVSKMS CVVVCRAAFG SVLKDQGSLA 200
    ELVKESLALA SGFELADLYP SSWLLNLLSL NKYRLQRMRR RLDHILDGFL 250
    EEHREKKSGE FGGEDIVDVL FRMQKGSDIK IPITSNCIKG FIFDTFSAGA 300
    ETSSTTISWA LSELMRNPAK MAKVQAEVRE ALKGKTVVDL SEVQELKYLR 350
    SVLKETLRLH PPFPLIPRQS REECEVNGYT IPAKTRIFIN VWAIGRDPQY 400
    WEDPDTFRPE RFDEVSRDFM GNDFEFIPFG AGRRICPGLH FGLANVEIPL 450
    AQLLYHFDWK LPQGMTDADL DMTETPGLSG PKKKNVCLVP TLYKSP 496
    Length:496
    Mass (Da):56,149
    Last modified:November 1, 1999 - v1
    Checksum:i06E4370F95EB91C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF124815 mRNA. Translation: AAD44150.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Mint condition - Issue 113 of January 2010

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF124815 mRNA. Translation: AAD44150.1 .

    3D structure databases

    ProteinModelPortali Q9XHE8.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-15424.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
      Lupien S., Karp F., Wildung M., Croteau R.
      Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-21; 176-200 AND 375-398, CATALYTIC ACTIVITY, FUNCTION.
    2. "A single amino acid substitution (F363I) converts the regiochemistry of the spearmint (-)-limonene hydroxylase from a C6- to a C3-hydroxylase."
      Schalk M., Croteau R.
      Proc. Natl. Acad. Sci. U.S.A. 97:11948-11953(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LEU-357; LEU-359; PHE-363; GLN-369 AND SER-370.
    3. "Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
      Wuest M., Little D.B., Schalk M., Croteau R.
      Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiC71DI_MENSP
    AccessioniPrimary (citable) accession number: Q9XHE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3