Q9XHE7 (C71DD_MENPI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 71D13
EC=1.14.13.47

Alternative name(s):
(-)-(4S)-Limonene-3-hydroxylase
Cytochrome P450 isoform PM17

Gene names

Name:

CYP71D13

Organism

Mentha piperita (Peppermint)

Taxonomic identifier

34256 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Lamiaceae › Nepetoideae › Mentheae › Mentha

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydroxylates (-)-(4S)-limonene to (-)-trans-isopiperitenol, a precursor of (-)-menthol, responsible for the cooling sensation of peppermint. Ref.1 Ref.2
Catalytic activity	--(S)-limonene + NADPH + O2 = (-)-trans-isopiperitenol + NADP+ + H2O. Ref.1 Ref.2
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	(S)-limonene 3-monooxygenase activity Inferred from direct assay Ref.1. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Cytochrome P450 71D13		PRO_0000389500
Regions
	Transmembrane	3 – 23	21	Helical; Signal-anchor for type II membrane protein; Potential
Sites
	Metal binding	439	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XHE7 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 402324F738F5949F	FASTA	500	56,601
	10 20 30 40 50 60 MELQISSAII ILVVTYTISL LIIKQWRKPK PQENLPPGPP KLPLIGHLHL LWGKLPQHAL 70 80 90 100 110 120 ASVAKQYGPV AHVQLGEVFS VVLSSREATK EAMKLVDPAC ADRFESIGTK IMWYDNDDII 130 140 150 160 170 180 FSPYSVHWRQ MRKICVSELL SARNVRSFGF IRQDEVSRLL GHLRSSAAAG EAVDLTERIA 190 200 210 220 230 240 TLTCSIICRA AFGSVIRDHE ELVELVKDAL SMASGFELAD MFPSSKLLNL LCWNKSKLWR 250 260 270 280 290 300 MRRRVDAILE AIVEEHKLKK SGEFGGEDII DVLFRMQKDS QIKVPITTNA IKAFIFDTFS 310 320 330 340 350 360 AGTETSSTTT LWVMAELMRN PEVMAKAQAE VRAALKGKTD WDVDDVQELK YMKSVVKETM 370 380 390 400 410 420 RMHPPIPLIP RSCREECEVN GYTIPNKARI MINVWSMGRN PLYWEKPETF WPERFDQVSR 430 440 450 460 470 480 DFMGNDFEFI PFGAGRRICP GLNFGLANVE VPLAQLLYHF DWKLAEGMNP SDMDMSEAEG 490 500 LTGIRKNNLL LVPTPYDPSS

References

[1]

"Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase." Lupien S., Karp F., Wildung M., Croteau R. Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY. Strain: cv. Black Mitcham.

[2]

"Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites." Wuest M., Little D.B., Schalk M., Croteau R. Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF124816 mRNA. Translation: AAD44151.1.
3D structure databases
ProteinModelPortal	Q9XHE7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-6762.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C71DD_MENPI

Accession

Primary (citable) accession number: Q9XHE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	November 1, 1999
Last modified:	May 29, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program