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Q9XHE7

- C71DD_MENPI

UniProt

Q9XHE7 - C71DD_MENPI

Protein

Cytochrome P450 71D13

Gene

CYP71D13

Organism
Mentha piperita (Peppermint)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Hydroxylates (-)-(4S)-limonene to (-)-trans-isopiperitenol, a precursor of (-)-menthol, responsible for the cooling sensation of peppermint.2 Publications

    Catalytic activityi

    --(S)-limonene + NADPH + O2 = (-)-trans-isopiperitenol + NADP+ + H2O.2 Publications

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. (S)-limonene 3-monooxygenase activity Source: UniProtKB
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-6762.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 71D13 (EC:1.14.13.47)
    Alternative name(s):
    (-)-(4S)-Limonene-3-hydroxylase
    Cytochrome P450 isoform PM17
    Gene namesi
    Name:CYP71D13
    OrganismiMentha piperita (Peppermint)
    Taxonomic identifieri34256 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Cytochrome P450 71D13PRO_0000389500Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XHE7.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XHE7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELQISSAII ILVVTYTISL LIIKQWRKPK PQENLPPGPP KLPLIGHLHL    50
    LWGKLPQHAL ASVAKQYGPV AHVQLGEVFS VVLSSREATK EAMKLVDPAC 100
    ADRFESIGTK IMWYDNDDII FSPYSVHWRQ MRKICVSELL SARNVRSFGF 150
    IRQDEVSRLL GHLRSSAAAG EAVDLTERIA TLTCSIICRA AFGSVIRDHE 200
    ELVELVKDAL SMASGFELAD MFPSSKLLNL LCWNKSKLWR MRRRVDAILE 250
    AIVEEHKLKK SGEFGGEDII DVLFRMQKDS QIKVPITTNA IKAFIFDTFS 300
    AGTETSSTTT LWVMAELMRN PEVMAKAQAE VRAALKGKTD WDVDDVQELK 350
    YMKSVVKETM RMHPPIPLIP RSCREECEVN GYTIPNKARI MINVWSMGRN 400
    PLYWEKPETF WPERFDQVSR DFMGNDFEFI PFGAGRRICP GLNFGLANVE 450
    VPLAQLLYHF DWKLAEGMNP SDMDMSEAEG LTGIRKNNLL LVPTPYDPSS 500
    Length:500
    Mass (Da):56,601
    Last modified:November 1, 1999 - v1
    Checksum:i402324F738F5949F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF124816 mRNA. Translation: AAD44151.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF124816 mRNA. Translation: AAD44151.1 .

    3D structure databases

    ProteinModelPortali Q9XHE7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-6762.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
      Lupien S., Karp F., Wildung M., Croteau R.
      Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
      Strain: cv. Black Mitcham.
    2. "Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
      Wuest M., Little D.B., Schalk M., Croteau R.
      Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiC71DD_MENPI
    AccessioniPrimary (citable) accession number: Q9XHE7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3