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Q9XHE7 (C71DD_MENPI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 71D13

EC=1.14.13.47
Alternative name(s):
(-)-(4S)-Limonene-3-hydroxylase
Cytochrome P450 isoform PM17
Gene names
Name:CYP71D13
OrganismMentha piperita (Peppermint)
Taxonomic identifier34256 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydroxylates (-)-(4S)-limonene to (-)-trans-isopiperitenol, a precursor of (-)-menthol, responsible for the cooling sensation of peppermint. Ref.1 Ref.2

Catalytic activity

--(S)-limonene + NADPH + O2 = (-)-trans-isopiperitenol + NADP+ + H2O. Ref.1 Ref.2

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Cytochrome P450 71D13
PRO_0000389500

Regions

Transmembrane3 – 2321Helical; Signal-anchor for type II membrane protein; Potential

Sites

Metal binding4391Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9XHE7 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 402324F738F5949F

FASTA50056,601
        10         20         30         40         50         60 
MELQISSAII ILVVTYTISL LIIKQWRKPK PQENLPPGPP KLPLIGHLHL LWGKLPQHAL 

        70         80         90        100        110        120 
ASVAKQYGPV AHVQLGEVFS VVLSSREATK EAMKLVDPAC ADRFESIGTK IMWYDNDDII 

       130        140        150        160        170        180 
FSPYSVHWRQ MRKICVSELL SARNVRSFGF IRQDEVSRLL GHLRSSAAAG EAVDLTERIA 

       190        200        210        220        230        240 
TLTCSIICRA AFGSVIRDHE ELVELVKDAL SMASGFELAD MFPSSKLLNL LCWNKSKLWR 

       250        260        270        280        290        300 
MRRRVDAILE AIVEEHKLKK SGEFGGEDII DVLFRMQKDS QIKVPITTNA IKAFIFDTFS 

       310        320        330        340        350        360 
AGTETSSTTT LWVMAELMRN PEVMAKAQAE VRAALKGKTD WDVDDVQELK YMKSVVKETM 

       370        380        390        400        410        420 
RMHPPIPLIP RSCREECEVN GYTIPNKARI MINVWSMGRN PLYWEKPETF WPERFDQVSR 

       430        440        450        460        470        480 
DFMGNDFEFI PFGAGRRICP GLNFGLANVE VPLAQLLYHF DWKLAEGMNP SDMDMSEAEG 

       490        500 
LTGIRKNNLL LVPTPYDPSS 

« Hide

References

[1]"Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
Lupien S., Karp F., Wildung M., Croteau R.
Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
Strain: cv. Black Mitcham.
[2]"Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
Wuest M., Little D.B., Schalk M., Croteau R.
Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF124816 mRNA. Translation: AAD44151.1.

3D structure databases

ProteinModelPortalQ9XHE7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-6762.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC71DD_MENPI
AccessionPrimary (citable) accession number: Q9XHE7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families