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Q9XHE7

- C71DD_MENPI

UniProt

Q9XHE7 - C71DD_MENPI

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Protein

Cytochrome P450 71D13

Gene

CYP71D13

Organism
Mentha piperita (Peppermint)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Hydroxylates (-)-(4S)-limonene to (-)-trans-isopiperitenol, a precursor of (-)-menthol, responsible for the cooling sensation of peppermint.2 Publications

Catalytic activityi

--(S)-limonene + NADPH + O2 = (-)-trans-isopiperitenol + NADP+ + H2O.2 Publications

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. (S)-limonene 3-monooxygenase activity Source: UniProtKB
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6762.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 71D13 (EC:1.14.13.47)
Alternative name(s):
(-)-(4S)-Limonene-3-hydroxylase
Cytochrome P450 isoform PM17
Gene namesi
Name:CYP71D13
OrganismiMentha piperita (Peppermint)
Taxonomic identifieri34256 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Cytochrome P450 71D13PRO_0000389500Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9XHE7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XHE7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELQISSAII ILVVTYTISL LIIKQWRKPK PQENLPPGPP KLPLIGHLHL
60 70 80 90 100
LWGKLPQHAL ASVAKQYGPV AHVQLGEVFS VVLSSREATK EAMKLVDPAC
110 120 130 140 150
ADRFESIGTK IMWYDNDDII FSPYSVHWRQ MRKICVSELL SARNVRSFGF
160 170 180 190 200
IRQDEVSRLL GHLRSSAAAG EAVDLTERIA TLTCSIICRA AFGSVIRDHE
210 220 230 240 250
ELVELVKDAL SMASGFELAD MFPSSKLLNL LCWNKSKLWR MRRRVDAILE
260 270 280 290 300
AIVEEHKLKK SGEFGGEDII DVLFRMQKDS QIKVPITTNA IKAFIFDTFS
310 320 330 340 350
AGTETSSTTT LWVMAELMRN PEVMAKAQAE VRAALKGKTD WDVDDVQELK
360 370 380 390 400
YMKSVVKETM RMHPPIPLIP RSCREECEVN GYTIPNKARI MINVWSMGRN
410 420 430 440 450
PLYWEKPETF WPERFDQVSR DFMGNDFEFI PFGAGRRICP GLNFGLANVE
460 470 480 490 500
VPLAQLLYHF DWKLAEGMNP SDMDMSEAEG LTGIRKNNLL LVPTPYDPSS
Length:500
Mass (Da):56,601
Last modified:November 1, 1999 - v1
Checksum:i402324F738F5949F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124816 mRNA. Translation: AAD44151.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124816 mRNA. Translation: AAD44151.1 .

3D structure databases

ProteinModelPortali Q9XHE7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-6762.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
    Lupien S., Karp F., Wildung M., Croteau R.
    Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
    Strain: cv. Black Mitcham.
  2. "Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
    Wuest M., Little D.B., Schalk M., Croteau R.
    Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiC71DD_MENPI
AccessioniPrimary (citable) accession number: Q9XHE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3