SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9XHE6

- C71DF_MENPI

UniProt

Q9XHE6 - C71DF_MENPI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Cytochrome P450 71D15
Gene
CYP71D15
Organism
Mentha piperita (Peppermint)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydroxylates (-)-(4S)-limonene to (-)-trans-isopiperitenol, a precursor of (-)-menthol, responsible for the cooling sensation of peppermint. Fluorinated substrate analogs are hydroxylated with the same regio- and stereochemistry.2 Publications

Catalytic activityi

--(S)-limonene + NADPH + O2 = (-)-trans-isopiperitenol + NADP+ + H2O.2 Publications

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. (S)-limonene 3-monooxygenase activity Source: UniProtKB
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6761.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 71D15 (EC:1.14.13.47)
Alternative name(s):
(-)-(4S)-Limonene-3-hydroxylase
Cytochrome P450 isoform PM2
Gene namesi
Name:CYP71D15
OrganismiMentha piperita (Peppermint)
Taxonomic identifieri34256 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi358 – 3581M → L: No effect on activity or regiospecificity. 1 Publication
Mutagenesisi360 – 3601M → L: Strong decrease of activity. 1 Publication
Mutagenesisi364 – 3641I → F: Loss of activity. 1 Publication
Mutagenesisi370 – 3701S → Q: No effect on activity or regiospecificity. 1 Publication
Mutagenesisi371 – 3711C → S: No effect on activity or regiospecificity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Cytochrome P450 71D15
PRO_0000389499Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9XHE6.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XHE6-1 [UniParc]FASTAAdd to Basket

« Hide

MELLQLWSAL IILVVTYTIS LLINQWRKPK PQGKFPPGPP KLPLIGHLHL    50
LWGKLPQHAL ASVAKEYGPV AHVQLGEVFS VVLSSREATK EAMKLVDPAC 100
ANRFESIGTR IMWYDNEDII FSPYSEHWRQ MRKICVSELL SSRNVRSFGF 150
IRQDEVSRLL RHLRSSAGAA VDMTERIETL TCSIICRAAF GSVIRDNAEL 200
VGLVKDALSM ASGFELADMF PSSKLLNLLC WNKSKLWRMR RRVDTILEAI 250
VDEHKFKKSG EFGGEDIIDV LFRMQKDTQI KVPITTNSIK AFIFDTFSAG 300
TETSSTTTLW VLAELMRNPA VMAKAQAEVR AALKEKTNWD VDDVQELKYM 350
KSVVKETMRM HPPIPLIPRS CREECVVNGY TIPNKARIMI NVWSMGRNPL 400
YWEKPDTFWP ERFDQVSKDF MGNDFEFVPF GAGRRICPGL NFGLANVEVP 450
LAQLLYHFDW KLAEGMKPSD MDMSEAEGLT GILKNNLLLV PTPYDPSS 498
Length:498
Mass (Da):56,532
Last modified:November 1, 1999 - v1
Checksum:iD78DDC2CD5C7EA48
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF124817 mRNA. Translation: AAD44152.1.
EU108698 mRNA. Translation: ABW86882.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Mint condition - Issue 113 of January 2010

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF124817 mRNA. Translation: AAD44152.1 .
EU108698 mRNA. Translation: ABW86882.1 .

3D structure databases

ProteinModelPortali Q9XHE6.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-6761.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
    Lupien S., Karp F., Wildung M., Croteau R.
    Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
    Strain: cv. Black Mitcham.
  2. "Isolation of full-length genes of menthol biosynthesis pathway from Mentha x piperita cv. Madhuras."
    Gupta M.K., Gupta S., Shasany A.K., Khanuja S.P.S.
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Madhuras.
  3. "A single amino acid substitution (F363I) converts the regiochemistry of the spearmint (-)-limonene hydroxylase from a C6- to a C3-hydroxylase."
    Schalk M., Croteau R.
    Proc. Natl. Acad. Sci. U.S.A. 97:11948-11953(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF MET-358; MET-360; ILE-364; SER-370 AND CYS-371.
  4. "Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
    Wuest M., Little D.B., Schalk M., Croteau R.
    Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiC71DF_MENPI
AccessioniPrimary (citable) accession number: Q9XHE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi