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Protein

Cytochrome P450 71D15

Gene

CYP71D15

Organism
Mentha piperita (Peppermint)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydroxylates (-)-(4S)-limonene to (-)-trans-isopiperitenol, a precursor of (-)-menthol, responsible for the cooling sensation of peppermint. Fluorinated substrate analogs are hydroxylated with the same regio- and stereochemistry.2 Publications

Catalytic activityi

--(S)-limonene + NADPH + O2 = (-)-trans-isopiperitenol + NADP+ + H2O.2 Publications

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. (S)-limonene 3-monooxygenase activity Source: UniProtKB
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6761.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 71D15 (EC:1.14.13.47)
Alternative name(s):
(-)-(4S)-Limonene-3-hydroxylase
Cytochrome P450 isoform PM2
Gene namesi
Name:CYP71D15
OrganismiMentha piperita (Peppermint)
Taxonomic identifieri34256 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi358 – 3581M → L: No effect on activity or regiospecificity. 1 Publication
Mutagenesisi360 – 3601M → L: Strong decrease of activity. 1 Publication
Mutagenesisi364 – 3641I → F: Loss of activity. 1 Publication
Mutagenesisi370 – 3701S → Q: No effect on activity or regiospecificity. 1 Publication
Mutagenesisi371 – 3711C → S: No effect on activity or regiospecificity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Cytochrome P450 71D15PRO_0000389499Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9XHE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XHE6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELLQLWSAL IILVVTYTIS LLINQWRKPK PQGKFPPGPP KLPLIGHLHL
60 70 80 90 100
LWGKLPQHAL ASVAKEYGPV AHVQLGEVFS VVLSSREATK EAMKLVDPAC
110 120 130 140 150
ANRFESIGTR IMWYDNEDII FSPYSEHWRQ MRKICVSELL SSRNVRSFGF
160 170 180 190 200
IRQDEVSRLL RHLRSSAGAA VDMTERIETL TCSIICRAAF GSVIRDNAEL
210 220 230 240 250
VGLVKDALSM ASGFELADMF PSSKLLNLLC WNKSKLWRMR RRVDTILEAI
260 270 280 290 300
VDEHKFKKSG EFGGEDIIDV LFRMQKDTQI KVPITTNSIK AFIFDTFSAG
310 320 330 340 350
TETSSTTTLW VLAELMRNPA VMAKAQAEVR AALKEKTNWD VDDVQELKYM
360 370 380 390 400
KSVVKETMRM HPPIPLIPRS CREECVVNGY TIPNKARIMI NVWSMGRNPL
410 420 430 440 450
YWEKPDTFWP ERFDQVSKDF MGNDFEFVPF GAGRRICPGL NFGLANVEVP
460 470 480 490
LAQLLYHFDW KLAEGMKPSD MDMSEAEGLT GILKNNLLLV PTPYDPSS
Length:498
Mass (Da):56,532
Last modified:November 1, 1999 - v1
Checksum:iD78DDC2CD5C7EA48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124817 mRNA. Translation: AAD44152.1.
EU108698 mRNA. Translation: ABW86882.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Mint condition - Issue 113 of January 2010

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124817 mRNA. Translation: AAD44152.1.
EU108698 mRNA. Translation: ABW86882.1.

3D structure databases

ProteinModelPortaliQ9XHE6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6761.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
    Lupien S., Karp F., Wildung M., Croteau R.
    Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
    Strain: cv. Black Mitcham.
  2. "Isolation of full-length genes of menthol biosynthesis pathway from Mentha x piperita cv. Madhuras."
    Gupta M.K., Gupta S., Shasany A.K., Khanuja S.P.S.
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Madhuras.
  3. "A single amino acid substitution (F363I) converts the regiochemistry of the spearmint (-)-limonene hydroxylase from a C6- to a C3-hydroxylase."
    Schalk M., Croteau R.
    Proc. Natl. Acad. Sci. U.S.A. 97:11948-11953(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF MET-358; MET-360; ILE-364; SER-370 AND CYS-371.
  4. "Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
    Wuest M., Little D.B., Schalk M., Croteau R.
    Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiC71DF_MENPI
AccessioniPrimary (citable) accession number: Q9XHE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 1, 1999
Last modified: January 7, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.