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Q9XHE6

- C71DF_MENPI

UniProt

Q9XHE6 - C71DF_MENPI

Protein

Cytochrome P450 71D15

Gene

CYP71D15

Organism
Mentha piperita (Peppermint)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Hydroxylates (-)-(4S)-limonene to (-)-trans-isopiperitenol, a precursor of (-)-menthol, responsible for the cooling sensation of peppermint. Fluorinated substrate analogs are hydroxylated with the same regio- and stereochemistry.2 Publications

    Catalytic activityi

    --(S)-limonene + NADPH + O2 = (-)-trans-isopiperitenol + NADP+ + H2O.2 Publications

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. (S)-limonene 3-monooxygenase activity Source: UniProtKB
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-6761.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 71D15 (EC:1.14.13.47)
    Alternative name(s):
    (-)-(4S)-Limonene-3-hydroxylase
    Cytochrome P450 isoform PM2
    Gene namesi
    Name:CYP71D15
    OrganismiMentha piperita (Peppermint)
    Taxonomic identifieri34256 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi358 – 3581M → L: No effect on activity or regiospecificity. 1 Publication
    Mutagenesisi360 – 3601M → L: Strong decrease of activity. 1 Publication
    Mutagenesisi364 – 3641I → F: Loss of activity. 1 Publication
    Mutagenesisi370 – 3701S → Q: No effect on activity or regiospecificity. 1 Publication
    Mutagenesisi371 – 3711C → S: No effect on activity or regiospecificity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498Cytochrome P450 71D15PRO_0000389499Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XHE6.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XHE6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELLQLWSAL IILVVTYTIS LLINQWRKPK PQGKFPPGPP KLPLIGHLHL    50
    LWGKLPQHAL ASVAKEYGPV AHVQLGEVFS VVLSSREATK EAMKLVDPAC 100
    ANRFESIGTR IMWYDNEDII FSPYSEHWRQ MRKICVSELL SSRNVRSFGF 150
    IRQDEVSRLL RHLRSSAGAA VDMTERIETL TCSIICRAAF GSVIRDNAEL 200
    VGLVKDALSM ASGFELADMF PSSKLLNLLC WNKSKLWRMR RRVDTILEAI 250
    VDEHKFKKSG EFGGEDIIDV LFRMQKDTQI KVPITTNSIK AFIFDTFSAG 300
    TETSSTTTLW VLAELMRNPA VMAKAQAEVR AALKEKTNWD VDDVQELKYM 350
    KSVVKETMRM HPPIPLIPRS CREECVVNGY TIPNKARIMI NVWSMGRNPL 400
    YWEKPDTFWP ERFDQVSKDF MGNDFEFVPF GAGRRICPGL NFGLANVEVP 450
    LAQLLYHFDW KLAEGMKPSD MDMSEAEGLT GILKNNLLLV PTPYDPSS 498
    Length:498
    Mass (Da):56,532
    Last modified:November 1, 1999 - v1
    Checksum:iD78DDC2CD5C7EA48
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF124817 mRNA. Translation: AAD44152.1.
    EU108698 mRNA. Translation: ABW86882.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Mint condition - Issue 113 of January 2010

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF124817 mRNA. Translation: AAD44152.1 .
    EU108698 mRNA. Translation: ABW86882.1 .

    3D structure databases

    ProteinModelPortali Q9XHE6.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-6761.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
      Lupien S., Karp F., Wildung M., Croteau R.
      Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
      Strain: cv. Black Mitcham.
    2. "Isolation of full-length genes of menthol biosynthesis pathway from Mentha x piperita cv. Madhuras."
      Gupta M.K., Gupta S., Shasany A.K., Khanuja S.P.S.
      Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Madhuras.
    3. "A single amino acid substitution (F363I) converts the regiochemistry of the spearmint (-)-limonene hydroxylase from a C6- to a C3-hydroxylase."
      Schalk M., Croteau R.
      Proc. Natl. Acad. Sci. U.S.A. 97:11948-11953(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF MET-358; MET-360; ILE-364; SER-370 AND CYS-371.
    4. "Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
      Wuest M., Little D.B., Schalk M., Croteau R.
      Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiC71DF_MENPI
    AccessioniPrimary (citable) accession number: Q9XHE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3