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Q9XHE6 (C71DF_MENPI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 71D15

EC=1.14.13.47
Alternative name(s):
(-)-(4S)-Limonene-3-hydroxylase
Cytochrome P450 isoform PM2
Gene names
Name:CYP71D15
OrganismMentha piperita (Peppermint)
Taxonomic identifier34256 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydroxylates (-)-(4S)-limonene to (-)-trans-isopiperitenol, a precursor of (-)-menthol, responsible for the cooling sensation of peppermint. Fluorinated substrate analogs are hydroxylated with the same regio- and stereochemistry. Ref.1 Ref.4

Catalytic activity

--(S)-limonene + NADPH + O2 = (-)-trans-isopiperitenol + NADP+ + H2O. Ref.1 Ref.4

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Cytochrome P450 71D15
PRO_0000389499

Regions

Transmembrane3 – 2321Helical; Signal-anchor for type II membrane protein; Potential

Sites

Metal binding4371Iron (heme axial ligand) By similarity

Experimental info

Mutagenesis3581M → L: No effect on activity or regiospecificity. Ref.3
Mutagenesis3601M → L: Strong decrease of activity. Ref.3
Mutagenesis3641I → F: Loss of activity. Ref.3
Mutagenesis3701S → Q: No effect on activity or regiospecificity. Ref.3
Mutagenesis3711C → S: No effect on activity or regiospecificity. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9XHE6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: D78DDC2CD5C7EA48

FASTA49856,532
        10         20         30         40         50         60 
MELLQLWSAL IILVVTYTIS LLINQWRKPK PQGKFPPGPP KLPLIGHLHL LWGKLPQHAL 

        70         80         90        100        110        120 
ASVAKEYGPV AHVQLGEVFS VVLSSREATK EAMKLVDPAC ANRFESIGTR IMWYDNEDII 

       130        140        150        160        170        180 
FSPYSEHWRQ MRKICVSELL SSRNVRSFGF IRQDEVSRLL RHLRSSAGAA VDMTERIETL 

       190        200        210        220        230        240 
TCSIICRAAF GSVIRDNAEL VGLVKDALSM ASGFELADMF PSSKLLNLLC WNKSKLWRMR 

       250        260        270        280        290        300 
RRVDTILEAI VDEHKFKKSG EFGGEDIIDV LFRMQKDTQI KVPITTNSIK AFIFDTFSAG 

       310        320        330        340        350        360 
TETSSTTTLW VLAELMRNPA VMAKAQAEVR AALKEKTNWD VDDVQELKYM KSVVKETMRM 

       370        380        390        400        410        420 
HPPIPLIPRS CREECVVNGY TIPNKARIMI NVWSMGRNPL YWEKPDTFWP ERFDQVSKDF 

       430        440        450        460        470        480 
MGNDFEFVPF GAGRRICPGL NFGLANVEVP LAQLLYHFDW KLAEGMKPSD MDMSEAEGLT 

       490 
GILKNNLLLV PTPYDPSS 

« Hide

References

[1]"Regiospecific cytochrome P450 limonene hydroxylases from mint (Mentha) species: cDNA isolation, characterization, and functional expression of (-)-4S-limonene-3-hydroxylase and (-)-4S-limonene-6-hydroxylase."
Lupien S., Karp F., Wildung M., Croteau R.
Arch. Biochem. Biophys. 368:181-192(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
Strain: cv. Black Mitcham.
[2]"Isolation of full-length genes of menthol biosynthesis pathway from Mentha x piperita cv. Madhuras."
Gupta M.K., Gupta S., Shasany A.K., Khanuja S.P.S.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Madhuras.
[3]"A single amino acid substitution (F363I) converts the regiochemistry of the spearmint (-)-limonene hydroxylase from a C6- to a C3-hydroxylase."
Schalk M., Croteau R.
Proc. Natl. Acad. Sci. U.S.A. 97:11948-11953(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF MET-358; MET-360; ILE-364; SER-370 AND CYS-371.
[4]"Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from mint (Mentha) species: evidence for catalysis within sterically constrained active sites."
Wuest M., Little D.B., Schalk M., Croteau R.
Arch. Biochem. Biophys. 387:125-136(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Mint condition - Issue 113 of January 2010

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF124817 mRNA. Translation: AAD44152.1.
EU108698 mRNA. Translation: ABW86882.1.

3D structure databases

ProteinModelPortalQ9XHE6.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-6761.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC71DF_MENPI
AccessionPrimary (citable) accession number: Q9XHE6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries