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Protein

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

GNTI

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Required for normal root growth and morphology.5 Publications

Miscellaneous

Creation of a second N-glycosylation site in mutant cgl1 C5/cgl1-1 interferes with protein folding and sequesters the protein for degradation in the endoplasmic reticulum.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein].

Cofactori

Mn2+By similarityNote: The cofactor is mostly bound to the substrate.By similarity

Kineticsi

the apparent KM is >3.0 mM for Man3-octyl.
  1. KM=0.14 mM for Man5-glycopeptide1 Publication
  2. KM=0.05 mM for UDP-GlcNAc1 Publication
  1. Vmax=3.42 µmol/min/mg enzyme toward Man5-glycopeptide1 Publication
  2. Vmax=0.09 µmol/min/mg enzyme toward Man3-octyl1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115SubstrateBy similarity1
Binding sitei144SubstrateBy similarity1
Binding sitei188SubstrateBy similarity1
Binding sitei210SubstrateBy similarity1
Metal bindingi211ManganeseBy similarity1
Active sitei287Proton acceptorSequence analysis1
Binding sitei318SubstrateBy similarity1

GO - Molecular functioni

  • alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • protein N-acetylglucosaminyltransferase activity Source: TAIR
  • transferase activity, transferring glycosyl groups Source: TAIR

GO - Biological processi

  • hyperosmotic response Source: TAIR
  • N-glycan processing Source: TAIR
  • protein glycosylation Source: TAIR

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT4G38240-MONOMER
ReactomeiR-ATH-964739 N-glycan trimming and elongation in the cis-Golgi
SABIO-RKiQ9XGM8
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT13 Glycosyltransferase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
Alternative name(s):
N-acetylglucosaminyltransferase I
Short name:
GlcNAcT-I
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Protein COMPLEX GLYCAN LESS 1
Gene namesi
Name:GNTI
Synonyms:CGL1
Ordered Locus Names:At4g38240
ORF Names:F20D10.360, F22I13.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G38240
TAIRilocus:2120993 AT4G38240

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini25 – 444LumenalSequence analysisAdd BLAST420

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants have an increased salt-sensitivity resulting in growth inhibition, aberrant root-tip morphology and callose accumulation.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi144D → N in cgl1 C5/cgl1-1; loss of activity due to degradation in the reticulum endoplasmic. 1
Mutagenesisi353T → V: Loss of glycosylation. No effect on localization and stability. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003561911 – 444Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseAdd BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi351N-linked (GlcNAc...) asparagine1

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9XGM8

PTM databases

SwissPalmiQ9XGM8

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ9XGM8 baseline and differential
GenevisibleiQ9XGM8 AT

Interactioni

Protein-protein interaction databases

BioGridi15261, 2 interactors
STRINGi3702.AT4G38240.1

Structurei

3D structure databases

ProteinModelPortaliQ9XGM8
SMRiQ9XGM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili61 – 92Sequence analysisAdd BLAST32

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1413 Eukaryota
ENOG410XQD8 LUCA
HOGENOMiHOG000247055
InParanoidiQ9XGM8
KOiK00726
OMAiQDPSLWC
OrthoDBiEOG09360A5Z
PhylomeDBiQ9XGM8

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004139 Glyco_trans_13
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR10468 PTHR10468, 1 hit
PfamiView protein in Pfam
PF03071 GNT-I, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9XGM8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARISCDLRF LLIPAAFMFI YIQMRLFQTQ SQYADRLSSA IESENHCTSQ
60 70 80 90 100
MRGLIDEVSI KQSRIVALED MKNRQDEELV QLKDLIQTFE KKGIAKLTQG
110 120 130 140 150
GQMPVAAVVV MACSRADYLE RTVKSVLTYQ TPVASKYPLF ISQDGSDQAV
160 170 180 190 200
KSKSLSYNQL TYMQHLDFEP VVTERPGELT AYYKIARHYK WALDQLFYKH
210 220 230 240 250
KFSRVIILED DMEIAPDFFD YFEAAASLMD RDKTIMAASS WNDNGQKQFV
260 270 280 290 300
HDPYALYRSD FFPGLGWMLK RSTWDELSPK WPKAYWDDWL RLKENHKGRQ
310 320 330 340 350
FIRPEVCRTY NFGEHGSSLG QFFSQYLEPI KLNDVTVDWK AKDLGYLTEG
360 370 380 390 400
NYTKYFSGLV RQARPIQGSD LVLKAQNIKD DVRIRYKDQV EFERIAGEFG
410 420 430 440
IFEEWKDGVP RTAYKGVVVF RIQTTRRVFL VGPDSVMQLG IRNS
Length:444
Mass (Da):51,634
Last modified:November 1, 1999 - v1
Checksum:iB3DDCF221C526336
GO

Sequence cautioni

The sequence CAB37480 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB37564 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80489 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti303R → A in CAC80700 (PubMed:10889259).Curated1
Sequence conflicti382V → D in CAC80700 (PubMed:10889259).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243198 mRNA Translation: CAB45521.1
AJ249881 mRNA Translation: CAC80700.1
AL035538 Genomic DNA Translation: CAB37564.1 Sequence problems.
AL035539 Genomic DNA Translation: CAB37480.1 Sequence problems.
AL161593 Genomic DNA Translation: CAB80489.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE86902.1
CP002687 Genomic DNA Translation: AEE86903.1
AY099838 mRNA Translation: AAM20689.1
BT000334 mRNA Translation: AAN15653.1
PIRiJC7084
T05651
RefSeqiNP_195537.2, NM_119986.3 [Q9XGM8-1]
NP_849517.1, NM_179186.1 [Q9XGM8-1]
UniGeneiAt.22245

Genome annotation databases

EnsemblPlantsiAT4G38240.1; AT4G38240.1; AT4G38240 [Q9XGM8-1]
AT4G38240.2; AT4G38240.2; AT4G38240 [Q9XGM8-1]
GeneIDi829981
GrameneiAT4G38240.1; AT4G38240.1; AT4G38240 [Q9XGM8-1]
AT4G38240.2; AT4G38240.2; AT4G38240 [Q9XGM8-1]
KEGGiath:AT4G38240

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMGAT1_ARATH
AccessioniPrimary (citable) accession number: Q9XGM8
Secondary accession number(s): Q8VWR5, Q9SVG1, Q9SZM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: November 1, 1999
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health