Q9XGA1 (KPRS4_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 4
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 4

Gene names

Name:

PRS4

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Ribose-phosphate pyrophosphokinase 4		PRO_0000141102
Regions
	Region	222 – 237	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	139	1	Magnesium Potential
	Metal binding	141	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9XGA1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 1B493F769430025C	FASTA	318	35,385
	10 20 30 40 50 60 MEKPNTKQVL LFYCVEAEEL ARKVAAQSPL ITLQSINWRS FDDGFPNLFI NNAQDIRGQH 70 80 90 100 110 120 VAFLAAFSSP AVIFEQLSVI YALPRLFVAS FTLVLPFFPT GSFERMEEEG DVATAFTMAR 130 140 150 160 170 180 ILSNIPVSRG GPTSVVIYDI HALQERFYFS DNVLPLFETG IPLLKQRLDQ LPDADKIVVA 190 200 210 220 230 240 FPDDGAWKRF HKQLDHFPMV VCAKVREGDK RIVRLKEGNP AGCHVVIVDD LVQSGGTLIE 250 260 270 280 290 300 CQKVLAAHGA TKVSAYVTHA VFPKNSFERF THKDDGSDKA FTYFWITDSC PRTVKSIANK 310 APFEVLSLAG SIADALQI

References

[1]	"Organellar and cytosolic localization of four phosphoribosyl diphosphate synthase isozymes in spinach." Krath B.N., Hove-Jensen B. Plant Physiol. 119:497-506(1999) [PubMed: 9952445] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ006943 mRNA. Translation: CAB43602.1.
3D structure databases
ProteinModelPortal	Q9XGA1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS4_SPIOL

Accession

Primary (citable) accession number: Q9XGA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 1, 1999
Last modified:	May 31, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program