Reviewed, UniProtKB/Swiss-Prot Q9XGA0 (KPRS3_SPIOL)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 3, mitochondrial
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 3

Gene names

Name:

PRS3

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 87	87	Mitochondrion Potential
	Chain	88 – 406	319	Ribose-phosphate pyrophosphokinase 3, mitochondrial		PRO_0000016846
Regions
	Region	309 – 324	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	226	1	Magnesium Potential
	Metal binding	228	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9XGA0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 9374BDD6FC499ABF	FASTA	406	45,437
	10 20 30 40 50 60 MAATPHRHLL QPCKNPAISS SETLKPSSSF SLSSNPSIPL IRRRLPTFRC DFQNRRKWMN 70 80 90 100 110 120 VDHHISKLNP IQIVPNSRRF QMSSNQENSV EFSKKVCLFY CPETKALAER IAAQSDAIQL 130 140 150 160 170 180 RSISWRTFED GFPNLFISNA QGIRGKHVAF LASFSSPGVI FEQLSVIYAL PKLFVASFKL 190 200 210 220 230 240 VLPFFPTGTS ERMEDEGDVA TAFTLARILS NIPISREGPT SLVTFDIHAL QERFYFGDNI 250 260 270 280 290 300 LPCFESGIPL LKKKLQQLPD SDNITIAFPD DGAWKRFHKQ LQHFPMIVCA KVREGDQRIV 310 320 330 340 350 360 RLKEGDPTGR HVVIVDDLVQ SGGTLIECQK VLAAHGAAKV SAYVTHGIFP NKSWERFKPD 370 380 390 400 TAGCPEEGMT HFWITDSCPL TVKMVKNRPP FEVISLAGSI AAALQI

References

[1]	"Organellar and cytosolic localization of four phosphoribosyl diphosphate synthase isozymes in spinach." Krath B.N., Hove-Jensen B. Plant Physiol. 119:497-506(1999) [PubMed: 9952445] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ006942 mRNA. Translation: CAB43601.1.
3D structure databases
SMR	Q9XGA0. Positions 96-405.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.6.1. 286.
Family and domain databases
InterPro	IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS3_SPIOL

Accession

Primary (citable) accession number: Q9XGA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)