Reviewed, UniProtKB/Swiss-Prot Q9XG99 (KPRS2_SPIOL)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 2, chloroplastic
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 2

Gene names

Name:

PRS2

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Chloroplast Potential
	Chain	43 – 395	353	Ribose-phosphate pyrophosphokinase 2, chloroplastic		PRO_0000016845
Regions
	Region	295 – 310	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	209	1	Magnesium Potential
	Metal binding	211	1	Magnesium Potential
	Metal binding	220	1	Magnesium Potential
	Metal binding	224	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9XG99-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 98F83217249F2175	FASTA	395	42,715
	10 20 30 40 50 60 MASPAPRSLS SSSSSSSSSF CPSISPPPRS PSRASLPFSV KCNTVEPLKL VNGKPSVPIL 70 80 90 100 110 120 DEQSLPKFLH SKRLESSVNR SNTRLKLFSG LANPALAKEV AWYMGLELGK VKIKRFADGE 130 140 150 160 170 180 IYVQLEESVR GCDVFIIQPT SPPANENLME LLIMIDACRR ASAKNITAVI PYFGYARADR 190 200 210 220 230 240 KTQGRESIAA KLVANLITEA GANRVLACDL HSGQSMGYFD IPVDHVYCQP VILDYLASKG 250 260 270 280 290 300 IASSDLVVVS PDVGGVARAR AFAKKLSDAP LAIVDKRRHA HNVAEVMNLI GDVKGKVAVM 310 320 330 340 350 360 LDDMIDTAGT ITKGAELLHE EGAREVYACC THAVFSPPAI ERLSSGCFQE VIITNTLPVA 370 380 390 EKNFFRQLTV LSTANLLGET IWRVHDDSSV SSIFQ

References

[1]	"Organellar and cytosolic localization of four phosphoribosyl diphosphate synthase isozymes in spinach." Krath B.N., Hove-Jensen B. Plant Physiol. 119:497-506(1999) [PubMed: 9952445] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ006941 mRNA. Translation: CAB43600.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKR based on UniProtKB P14193.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.6.1. 286.
Family and domain databases
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_SPIOL

Accession

Primary (citable) accession number: Q9XG99

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 1, 1999
Last modified:	September 22, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)