Reviewed, UniProtKB/Swiss-Prot Q9XG83 (G2OX_PHACN)

Names and origin

Protein names

Recommended name:
    Gibberellin 2-beta-dioxygenase
    EC=1.14.11.13
Alternative name(s):
    Gibberellin 2-beta-hydroxylase
    Gibberellin 2-oxidase
    GA 2-oxidase

Gene names

Name:

GA2OX1

Organism

Phaseolus coccineus (Scarlet runner bean)

Taxonomic identifier

3886 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8.
Catalytic activity	Gibberellin 1 + 2-oxoglutarate + O(2) = 2-beta-hydroxygibberellin 1 + succinate + CO(2).
Cofactor	Iron By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	gibberellin 2-beta-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Gibberellin 2-beta-dioxygenase		PRO_0000067310
Sites
	Active site	271	1	Potential
	Metal binding	204	1	Iron By similarity
	Metal binding	206	1	Iron By similarity
	Metal binding	261	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XG83-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 00CDEBFD2C23D8A5	FASTA	332	37,150
	10 20 30 40 50 60 MVVLSQPALN QFFLLKPFKS TPLFTGIPVV DLTHPDAKNL IVNACRDFGF FKLVNHGVPL 70 80 90 100 110 120 ELMANLENEA LRFFKKSQSE KDRAGPPDPF GYGSKRIGPN GDVGWVEYLL LNTNPDVISP 130 140 150 160 170 180 KSLCIFRENP HHFRAVVENY ITAVKNMCYA VLELMAEGLG IRQRNTLSRL LKDEKSDSCF 190 200 210 220 230 240 RLNHYPPCPE VQALNRNLVG FGEHTDPQII SVLRSNSTSG LQICLTDGTW VSVPPDQTSF 250 260 270 280 290 300 FINVGDALQV MTNGRFKSVK HRVLADTTKS RLSMIYFGGP ALSENIAPLP SVMLKGEECL 310 320 330 YKEFTWCEYK KAAYTSRLAD NRLAPFQKSA AD

References

[1]

"Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation." Thomas S.G., Phillips A.L., Hedden P. Proc. Natl. Acad. Sci. U.S.A. 96:4698-4703(1999) [PubMed: 10200325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ132438 mRNA. Translation: CAB41036.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11631.
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. IPR002283. Isopenicillin-N_synthase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

G2OX_PHACN

Accession

Primary (citable) accession number: Q9XG83

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	November 1, 1999
Last modified:	November 4, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)