PLA2-II

Functionⁱ

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Releases lysophospholipids (LPLs) and free fatty acids (FFAs) from membrane phospholipids in response to hormones and other external stimuli.By similarity

Catalytic activityⁱ

Phosphatidylcholine + H₂O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactorⁱ

Ca²⁺Note: Binds 1 Ca²⁺ ion per subunit.

Sites

Feature key	Position(s)	Length	Description
Metal bindingⁱ	62 – 62	1	Calcium; via carbonyl oxygen
Metal bindingⁱ	64 – 64	1	Calcium; via carbonyl oxygen
Metal bindingⁱ	67 – 67	1	Calcium; via carbonyl oxygen
Active siteⁱ	86 – 86	1	PROSITE-ProRule annotation Manual assertion according to rulesⁱ PROSITE-ProRule:PRU10035
Metal bindingⁱ	87 – 87	1	Calcium

GO - Molecular functionⁱ

calcium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 19457861
lipid binding
Source: UniProtKB
Inferred from direct assayⁱ
- 19457861
phospholipase A2 activity Source: UniProtKB-EC

Enzyme and pathway databases

BRENDAⁱ	3.1.1.4. 4460.

BRENDAⁱ

3.1.1.4. 4460.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable phospholipase A2 homolog 2 (EC:3.1.1.4)
Gene namesⁱ	Name:PLA2-II Ordered Locus Names:Os03g0261100, LOC_Os03g15460 ORF Names:OsJ_10213
Organismⁱ	Oryza sativa subsp. japonica (Rice)
Taxonomic identifierⁱ	39947 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › Liliopsida › Petrosaviidae › commelinids › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa
Proteomesⁱ	UP000059680 Componentⁱ: Chromosome 3

Subcellular locationⁱ

Secreted Curated

GO - Cellular componentⁱ

extracellular region Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Signal peptideⁱ	1 – 25	25	Sequence analysis			Add BLAST
Chainⁱ	26 – 153	128	Probable phospholipase A2 homolog 2		PRO_5000065168	Add BLAST

Amino acid modifications

Feature key	Position(s)	Description
Disulfide bondⁱ	42 ↔ 70	1 Publication Manual assertion based on experiment inⁱ Ref.6 "Crystal structure of a class XIB phospholipase A2 (PLA2): rice (oryza sativa) isoform-2 pla2 and an octanoate complex." Guy J.E., Staahl U., Lindqvist Y. J. Biol. Chem. 284:19371-19379(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-153 IN COMPLEX WITH CALCIUM IONS AND SUBSTRATE ANALOG, DISULFIDE BONDS.
Disulfide bondⁱ	46 ↔ 76	1 Publication Manual assertion based on experiment inⁱ Ref.6 "Crystal structure of a class XIB phospholipase A2 (PLA2): rice (oryza sativa) isoform-2 pla2 and an octanoate complex." Guy J.E., Staahl U., Lindqvist Y. J. Biol. Chem. 284:19371-19379(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-153 IN COMPLEX WITH CALCIUM IONS AND SUBSTRATE ANALOG, DISULFIDE BONDS.
Disulfide bondⁱ	51 ↔ 123	1 Publication Manual assertion based on experiment inⁱ Ref.6 "Crystal structure of a class XIB phospholipase A2 (PLA2): rice (oryza sativa) isoform-2 pla2 and an octanoate complex." Guy J.E., Staahl U., Lindqvist Y. J. Biol. Chem. 284:19371-19379(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-153 IN COMPLEX WITH CALCIUM IONS AND SUBSTRATE ANALOG, DISULFIDE BONDS.
Disulfide bondⁱ	63 ↔ 83	1 Publication Manual assertion based on experiment inⁱ Ref.6 "Crystal structure of a class XIB phospholipase A2 (PLA2): rice (oryza sativa) isoform-2 pla2 and an octanoate complex." Guy J.E., Staahl U., Lindqvist Y. J. Biol. Chem. 284:19371-19379(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-153 IN COMPLEX WITH CALCIUM IONS AND SUBSTRATE ANALOG, DISULFIDE BONDS.
Disulfide bondⁱ	82 ↔ 109	1 Publication Manual assertion based on experiment inⁱ Ref.6 "Crystal structure of a class XIB phospholipase A2 (PLA2): rice (oryza sativa) isoform-2 pla2 and an octanoate complex." Guy J.E., Staahl U., Lindqvist Y. J. Biol. Chem. 284:19371-19379(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-153 IN COMPLEX WITH CALCIUM IONS AND SUBSTRATE ANALOG, DISULFIDE BONDS.
Disulfide bondⁱ	89 ↔ 102	1 Publication Manual assertion based on experiment inⁱ Ref.6 "Crystal structure of a class XIB phospholipase A2 (PLA2): rice (oryza sativa) isoform-2 pla2 and an octanoate complex." Guy J.E., Staahl U., Lindqvist Y. J. Biol. Chem. 284:19371-19379(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-153 IN COMPLEX WITH CALCIUM IONS AND SUBSTRATE ANALOG, DISULFIDE BONDS.

Keywords - PTMⁱ

Disulfide bond

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	Q9XG81. baseline and differential.
Genevisibleⁱ	Q9XG81. OS.

Structureⁱ

Secondary structure

1

153

Legend: HelixTurnBeta strand

Show more details

Feature key	Position(s)	Length	Description
Helixⁱ	28 – 30	3	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG7
Beta strandⁱ	39 – 41	3	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG9
Beta strandⁱ	47 – 50	4	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG7
Turnⁱ	54 – 56	3	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG7
Beta strandⁱ	58 – 60	3	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG7
Helixⁱ	78 – 91	14	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG7
Turnⁱ	92 – 95	4	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG7
Helixⁱ	100 – 110	11	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG7
Helixⁱ	125 – 149	25	Combined sources Manual assertion inferred from combination of experimental and computational evidenceⁱ PDB:2WG7

3D structure databases

Select the link destinations:
PDBeⁱ
RCSB PDBⁱ
PDBjⁱ

Links Updated

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WG7	X-ray	2.00	A/B	25-153	[»]
2WG8	X-ray	2.30	A/B/C	26-153	[»]
2WG9	X-ray	2.00	A/B	25-153	[»]

ModBaseⁱ

Search...

MobiDBⁱ

Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9XG81.

EvolutionaryTraceⁱ

Q9XG81.

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the phospholipase A2 family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

InParanoidⁱ	Q9XG81.
OMAⁱ	VHDHCVD.
OrthoDBⁱ	EOG09360R76.

Family and domain databases

Gene3Dⁱ	1.20.90.10. 1 hit.
InterProⁱ	IPR001211. PLipase_A2. IPR016090. PLipase_A2_dom. IPR033113. PLipase_A2_His_AS. [Graphical view]
PANTHERⁱ	PTHR11716. PTHR11716. 1 hit.
SUPFAMⁱ	SSF48619. SSF48619. 1 hit.
PROSITEⁱ	PS00118. PA2_HIS. 1 hit. [Graphical view]

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9XG81-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRFFLKLAPR CSVLLLLLLV TASRGLNIGD LLGSTPAKDQ GCSRTCESQF 
        60         70         80         90        100
CTIAPLLRYG KYCGILYSGC PGERPCDALD ACCMVHDHCV DTHNDDYLNT 
       110        120        130        140        150
MCNENLLSCI DRVSGATFPG NKCNVGQTAS VIRGVIETAV FAGKILHKRD 

DGQ

Length:153

Mass (Da):16,578

Last modified:November 1, 1999 - v1

Checksum:ⁱC9AC0BDB1F2885F6

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ238117 mRNA. Translation: CAB40842.1. AP008209 Genomic DNA. Translation: BAF11534.1. AP014959 Genomic DNA. Translation: BAS83356.1. CM000140 Genomic DNA. Translation: EEE58732.1.
RefSeqⁱ	XP_015629036.1. XM_015773550.1.
UniGeneⁱ	Os.4604.

Genome annotation databases

EnsemblPlantsⁱ	OS03T0261100-01; OS03T0261100-01; OS03G0261100.
GeneIDⁱ	4332319.
Grameneⁱ	OS03T0261100-01; OS03T0261100-01; OS03G0261100.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ238117 mRNA. Translation: CAB40842.1. AP008209 Genomic DNA. Translation: BAF11534.1. AP014959 Genomic DNA. Translation: BAS83356.1. CM000140 Genomic DNA. Translation: EEE58732.1.
RefSeqⁱ	XP_015629036.1. XM_015773550.1.
UniGeneⁱ	Os.4604.

3D structure databases

Select the link destinations:
PDBeⁱ
RCSB PDBⁱ
PDBjⁱ

Links Updated

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WG7	X-ray	2.00	A/B	25-153	[»]
2WG8	X-ray	2.30	A/B/C	26-153	[»]
2WG9	X-ray	2.00	A/B	25-153	[»]

ModBaseⁱ

Search...

MobiDBⁱ

Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	OS03T0261100-01; OS03T0261100-01; OS03G0261100.
GeneIDⁱ	4332319.
Grameneⁱ	OS03T0261100-01; OS03T0261100-01; OS03G0261100.

Phylogenomic databases

InParanoidⁱ	Q9XG81.
OMAⁱ	VHDHCVD.
OrthoDBⁱ	EOG09360R76.

Enzyme and pathway databases

BRENDAⁱ	3.1.1.4. 4460.

BRENDAⁱ

3.1.1.4. 4460.

Miscellaneous databases

EvolutionaryTraceⁱ	Q9XG81.

EvolutionaryTraceⁱ

Q9XG81.

Gene expression databases

ExpressionAtlasⁱ	Q9XG81. baseline and differential.
Genevisibleⁱ	Q9XG81. OS.

Family and domain databases

Gene3Dⁱ	1.20.90.10. 1 hit.
InterProⁱ	IPR001211. PLipase_A2. IPR016090. PLipase_A2_dom. IPR033113. PLipase_A2_His_AS. [Graphical view]
PANTHERⁱ	PTHR11716. PTHR11716. 1 hit.
SUPFAMⁱ	SSF48619. SSF48619. 1 hit.
PROSITEⁱ	PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ

PLA22_ORYSJ

Accessionⁱ

Primary (citable) accession number: Q9XG81
Secondary accession number(s): A0A0P0VVN9, Q0DTA4

Entry historyⁱ

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	November 1, 1999
Last modified:	September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry statusⁱ

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations
SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9XG81 (PLA22_ORYSJ)

UniProt

Q9XG81 - PLA22_ORYSJ

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Probable phospholipase A2 homolog 2

Select a section on the left to see content.

<p>Provides any useful information about the protein, mostly biological knowledge.</p><p><a href='../manual/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>Provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.</p><p><a href='../manual/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>Provides information on the location and the topology of the mature protein in the cell.</p><p><a href='../manual/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

<p>Provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.</p><p><a href='../manual/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>Provides information on the tertiary and secondary structure of a protein.</p><p><a href='../manual/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>Provides information on sequence similarities with other proteins and the domain(s) present in a protein.</p><p><a href='../manual/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>Provides information about the sequence similarity with other proteins.</p><p><a href='../manual/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>Displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.</p><p><a href='../manual/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>Is used to point to information related to entries and found in data collections other than UniProtKB.</p><p><a href='../manual/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>Contains any relevant information that doesn’t fit in any other defined sections</p><p><a href='../manual/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ