Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

12-oxophytodienoate reductase 1

Gene

OPR1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically cleaves olefinic bonds in alpha,beta-unsaturated carbonyls and may be involved in detoxification or modification of these reactive compounds. May be involved in the biosynthesis or metabolism of oxylipin signaling molecules. In vitro, reduces 9R,13R-12-oxophyodienoic acid (9R,13R-OPDA) to 9R,13R-OPC-8:0, but not 9S,13S-OPDA, the natural precursor of jasmonic acid. Also reduces N-ethylmaleimide and maleic acid.1 Publication

Catalytic activityi

8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH.1 Publication

Cofactori

FMN2 Publications

Kineticsi

The highest catalytic efficiency was observed with N-ethylmaleimide.

  1. KM=8 µM for N-ethylmaleimide (at pH 7.0 and 25 degrees Celsius)
  2. KM=47 µM for maleic acid (at pH 7.0 and 25 degrees Celsius)
  3. KM=15 µM for OPDA (at pH 7.0 and 25 degrees Celsius)

    Pathwayi: oxylipin biosynthesis

    This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei68FMN; via amide nitrogen1 Publication1
    Binding sitei110FMN1 Publication1
    Binding sitei143Substrate1 Publication1
    Active sitei192Proton donor1
    Binding sitei239FMN1 Publication1
    Binding sitei279Substrate; via amide nitrogenBy similarity1
    Binding sitei309FMN; via amide nitrogen1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi35 – 37FMN1 Publication3
    Nucleotide bindingi330 – 331FMN1 Publication2

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

    Keywords - Ligandi

    Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    BRENDAi1.3.1.42. 3101.
    UniPathwayiUPA00382.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    12-oxophytodienoate reductase 1 (EC:1.3.1.42)
    Alternative name(s):
    12-oxophytodienoate-10,11-reductase 1
    Short name:
    OPDA-reductase 1
    LeOPR1
    Gene namesi
    Name:OPR1
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    Proteomesi
    • UP000004994 Componenti: Chromosome 10

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001944881 – 37612-oxophytodienoate reductase 1Add BLAST376

    Proteomic databases

    PaxDbiQ9XG54.

    Expressioni

    Tissue specificityi

    Constitutively expressed in roots, leaves, cotyledons, cells culture and to a lower extent in flowers.1 Publication

    Inductioni

    Seems to not be influenced by wounding.

    Interactioni

    Protein-protein interaction databases

    STRINGi4081.Solyc10g086220.1.1.

    Structurei

    Secondary structure

    1376
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi15 – 17Combined sources3
    Beta strandi20 – 22Combined sources3
    Beta strandi25 – 28Combined sources4
    Beta strandi30 – 33Combined sources4
    Helixi42 – 44Combined sources3
    Helixi48 – 56Combined sources9
    Beta strandi63 – 65Combined sources3
    Beta strandi69 – 72Combined sources4
    Helixi73 – 75Combined sources3
    Helixi87 – 102Combined sources16
    Beta strandi106 – 112Combined sources7
    Turni120 – 122Combined sources3
    Helixi124 – 126Combined sources3
    Beta strandi130 – 134Combined sources5
    Beta strandi145 – 149Combined sources5
    Turni159 – 161Combined sources3
    Helixi162 – 178Combined sources17
    Beta strandi182 – 188Combined sources7
    Helixi193 – 198Combined sources6
    Turni200 – 202Combined sources3
    Beta strandi210 – 212Combined sources3
    Helixi213 – 231Combined sources19
    Helixi233 – 235Combined sources3
    Beta strandi236 – 240Combined sources5
    Turni246 – 248Combined sources3
    Helixi254 – 265Combined sources12
    Helixi266 – 268Combined sources3
    Beta strandi271 – 276Combined sources6
    Helixi294 – 299Combined sources6
    Beta strandi304 – 309Combined sources6
    Helixi312 – 320Combined sources9
    Beta strandi325 – 330Combined sources6
    Helixi331 – 335Combined sources5
    Helixi339 – 345Combined sources7
    Helixi354 – 356Combined sources3
    Turni364 – 366Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1ICPX-ray1.90A/B1-376[»]
    1ICQX-ray2.00A/B1-376[»]
    1ICSX-ray2.30A/B1-376[»]
    3HGRX-ray2.30A/B1-376[»]
    ProteinModelPortaliQ9XG54.
    SMRiQ9XG54.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9XG54.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni187 – 190Substrate-binding4

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG0134. Eukaryota.
    COG1902. LUCA.
    InParanoidiQ9XG54.
    KOiK05894.
    OMAiECTESLV.
    OrthoDBiEOG09360FPQ.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001155. OxRdtase_FMN_N.
    [Graphical view]
    PfamiPF00724. Oxidored_FMN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XG54-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MENKVVEEKQ VDKIPLMSPC KMGKFELCHR VVLAPLTRQR SYGYIPQPHA
    60 70 80 90 100
    ILHYSQRSTN GGLLIGEATV ISETGIGYKD VPGIWTKEQV EAWKPIVDAV
    110 120 130 140 150
    HAKGGIFFCQ IWHVGRVSNK DFQPNGEDPI SCTDRGLTPQ IRSNGIDIAH
    160 170 180 190 200
    FTRPRRLTTD EIPQIVNEFR VAARNAIEAG FDGVEIHGAH GYLIDQFMKD
    210 220 230 240 250
    QVNDRSDKYG GSLENRCRFA LEIVEAVANE IGSDRVGIRI SPFAHYNEAG
    260 270 280 290 300
    DTNPTALGLY MVESLNKYDL AYCHVVEPRM KTAWEKIECT ESLVPMRKAY
    310 320 330 340 350
    KGTFIVAGGY DREDGNRALI EDRADLVAYG RLFISNPDLP KRFELNAPLN
    360 370
    KYNRDTFYTS DPIVGYTDYP FLETMT
    Length:376
    Mass (Da):42,415
    Last modified:November 1, 1999 - v1
    Checksum:i83CFBEAC7CBC7A8F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ242551 mRNA. Translation: CAB43506.1.
    RefSeqiNP_001234781.1. NM_001247852.1.
    UniGeneiLes.22.

    Genome annotation databases

    EnsemblPlantsiSolyc10g086220.1.1; Solyc10g086220.1.1; Solyc10g086220.1.
    GeneIDi544239.
    GrameneiSolyc10g086220.1.1; Solyc10g086220.1.1; Solyc10g086220.1.
    KEGGisly:544239.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ242551 mRNA. Translation: CAB43506.1.
    RefSeqiNP_001234781.1. NM_001247852.1.
    UniGeneiLes.22.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1ICPX-ray1.90A/B1-376[»]
    1ICQX-ray2.00A/B1-376[»]
    1ICSX-ray2.30A/B1-376[»]
    3HGRX-ray2.30A/B1-376[»]
    ProteinModelPortaliQ9XG54.
    SMRiQ9XG54.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi4081.Solyc10g086220.1.1.

    Proteomic databases

    PaxDbiQ9XG54.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiSolyc10g086220.1.1; Solyc10g086220.1.1; Solyc10g086220.1.
    GeneIDi544239.
    GrameneiSolyc10g086220.1.1; Solyc10g086220.1.1; Solyc10g086220.1.
    KEGGisly:544239.

    Phylogenomic databases

    eggNOGiKOG0134. Eukaryota.
    COG1902. LUCA.
    InParanoidiQ9XG54.
    KOiK05894.
    OMAiECTESLV.
    OrthoDBiEOG09360FPQ.

    Enzyme and pathway databases

    UniPathwayiUPA00382.
    BRENDAi1.3.1.42. 3101.

    Miscellaneous databases

    EvolutionaryTraceiQ9XG54.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001155. OxRdtase_FMN_N.
    [Graphical view]
    PfamiPF00724. Oxidored_FMN. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiOPR1_SOLLC
    AccessioniPrimary (citable) accession number: Q9XG54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: November 1, 1999
    Last modified: November 2, 2016
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.