Reviewed, UniProtKB/Swiss-Prot Q9XFW4 (LPAT2_BRANA)

Names and origin

Protein names

Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase 2
    EC=2.3.1.51
Alternative name(s):
    Lysophosphatidyl acyltransferase 2

Gene names

Name:	LPAT2
Synonyms:	LPAAT2

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	1-acyl-sn-glycerol-3-phosphate acyltransferase 2		PRO_0000208180
Regions
	Transmembrane	2 – 22	21	Potential
	Transmembrane	305 – 325	21	Potential
	Transmembrane	333 – 353	21	Potential
	Motif	91 – 96	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q9XFW4-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F1446E1B30009C37	FASTA	390	43,771
	10 20 30 40 50 60 MAMAAAVIVP LGILFFISGL VVNLLQAVCY VLVRPMSKNT YRKINRVVAE TLWLELVWIV 70 80 90 100 110 120 DWWAGVKIQV FADDETFNRM GKEHALVVCN HRSDIDWLVG WILAQRSGCL GSALAVMKKS 130 140 150 160 170 180 SKFLPVIGWS MWFSEYLFLE RNWAKDESTL QSGLQRLNDF PRPFWLALFV EGTRFTEAKL 190 200 210 220 230 240 KAAQEYAASS ELPVPRNVLI PRTKGFVSAV SNMRSFVPAI YDMTVAIPKT SPPPTMLRLF 250 260 270 280 290 300 KGQPSVVHVH IKCHSMKDLP EPEDEIAQWC RDQFVAKDAL LDKHIAADTF PGQKEQNIGR 310 320 330 340 350 360 PIKSLAVVVS WACLLTLGAM KFLHWSNLFS SWKGIALSAF GLGIITLCMQ ILIRSSQSER 370 380 390 STPAKVAPAK PKDNHQSGPS SQTEVEEKQK

References

[1]	"A cDNA encoding a microsomal 1-acylglycerol-3-phosphate acyltransferase of Brassica napus L." Graefin zu Muenster A., Wolter F.P., Frentzen M. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Silique.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z95637 mRNA. Translation: CAB09138.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.51. 393.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPAT2_BRANA

Accession

Primary (citable) accession number: Q9XFW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	November 1, 1999
Last modified:	October 13, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)