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Reviewed, UniProtKB/Swiss-Prot Q9XFW3 (MDHG2_BRANA)

Last modified June 16, 2009. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Malate dehydrogenase 2, glyoxysomal
    EC=1.1.1.37
Gene names
Name: MDH2
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

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Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

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Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: InterPro

glyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

malate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3838Glyoxysome Potential
Chain39 – 358320Malate dehydrogenase 2, glyoxysomal
PRO_0000018637

Regions

Nucleotide binding53 – 597NAD By similarity
Nucleotide binding162 – 1643NAD By similarity

Sites

Active site2221Proton acceptor By similarity
Binding site791NAD By similarity
Binding site1261Substrate By similarity
Binding site1321Substrate By similarity
Binding site1391NAD By similarity
Binding site1641Substrate By similarity
Binding site1981Substrate By similarity
Binding site2731NAD By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9XFW3-1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: B73B2CB3DC1D2877

FASTA35837,725
        10         20         30         40         50         60 
MEFRGDANKR IAMISAHLQP SFTPQMEAKN SVMGRENCRA KGGNPGFKVA ILGAAGGIGQ 

        70         80         90        100        110        120 
SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR GFLGAKQLED ALTGMDLVII 

       130        140        150        160        170        180 
PAGVPRKPGM TRDDLFKINA GIVKTLCEGV AKCCPNAIVN LISNPVNSTV AIAAEVFKKA 

       190        200        210        220        230        240 
GTYDPKKLLG VTTLDVARAN TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF 

       250        260        270        280        290        300 
TPSEIEYLTN RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV 

       310        320        330        340        350 
ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ KGVDFIRK

« Hide

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References

[1]"Two genes encoding microbody malate dehydrogenase from Brassica napus."
Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

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Cross-references

Sequence databases

AJ242713 Genomic DNA. Translation: CAB43995.1.

3D structure databases

HSSPHSSP built from PDB template 1MLD based on UniProtKB P00346.
SMRQ9XFW3. Positions 46-357.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.1.1.37. 393.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_NAD-dep_euk_g_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR11540:SF1. MDH_euk_g_bac. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDHG2_BRANA
AccessionPrimary (citable) accession number: Q9XFW3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents