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Q9XFW3

- MDHG2_BRANA

UniProt

Q9XFW3 - MDHG2_BRANA

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Protein

Malate dehydrogenase 2, glyoxysomal

Gene

MDH2

Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791NADBy similarity
Binding sitei126 – 1261SubstratePROSITE-ProRule annotation
Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
Binding sitei139 – 1391NADBy similarity
Binding sitei164 – 1641SubstratePROSITE-ProRule annotation
Binding sitei198 – 1981SubstratePROSITE-ProRule annotation
Active sitei222 – 2221Proton acceptorBy similarity
Binding sitei273 – 2731NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 597NADBy similarity
Nucleotide bindingi162 – 1643NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase 2, glyoxysomal (EC:1.1.1.37)
Gene namesi
Name:MDH2
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838GlyoxysomeSequence AnalysisAdd
BLAST
Chaini39 – 358320Malate dehydrogenase 2, glyoxysomalPRO_0000018637Add
BLAST

Proteomic databases

PRIDEiQ9XFW3.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9XFW3.
SMRiQ9XFW3. Positions 46-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XFW3-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MEFRGDANKR IAMISAHLQP SFTPQMEAKN SVMGRENCRA KGGNPGFKVA
60 70 80 90 100
ILGAAGGIGQ SLSLLMKMNP LVSLLHLYDV VNAPGVTADV SHMDTGAVVR
110 120 130 140 150
GFLGAKQLED ALTGMDLVII PAGVPRKPGM TRDDLFKINA GIVKTLCEGV
160 170 180 190 200
AKCCPNAIVN LISNPVNSTV AIAAEVFKKA GTYDPKKLLG VTTLDVARAN
210 220 230 240 250
TFVAEVLGLD PREVDVPVVG GHAGVTILPL LSQVKPPSSF TPSEIEYLTN
260 270 280 290 300
RIQNGGTEVV EAKAGAGSAT LSMAYAAAKF ADACLRGLRG DANVIECSFV
310 320 330 340 350
ASQVTELAFF ATKVRLGRTG AEEVFQLGPL NEYERVGLEK AKEELAGSIQ

KGVDFIRK
Length:358
Mass (Da):37,725
Last modified:November 1, 1999 - v1
Checksum:iB73B2CB3DC1D2877
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ242713 Genomic DNA. Translation: CAB43995.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ242713 Genomic DNA. Translation: CAB43995.1 .

3D structure databases

ProteinModelPortali Q9XFW3.
SMRi Q9XFW3. Positions 46-357.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9XFW3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Two genes encoding microbody malate dehydrogenase from Brassica napus."
    Imhoff U., Voetz M., Wingender R., Schnabl H., Wolf N.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMDHG2_BRANA
AccessioniPrimary (citable) accession number: Q9XFW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3