Q9XFT3 (PSBQ1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oxygen-evolving enhancer protein 3-1, chloroplastic
Short name=OEE3

Alternative name(s):
16 kDa subunit of oxygen evolving system of photosystem II
OEC 16 kDa subunit

Gene names

Name:	PSBQ1
Synonyms:	PSBQ, PSBQA
Ordered Locus Names:	At4g21280
ORF Names:	F7J7.220, T6K22.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for photosystem II assembly/stability and photoautotrophic growth under low light conditions. Ref.8
Subcellular location	Plastid › chloroplast thylakoid membrane. Note: Associated with the photosystem II complex.
Tissue specificity	Expressed in green tissue, with high steady-state mRNA levels in leaves. Not expressed in roots. Ref.7
Induction	By light. Ref.1 Ref.7
Sequence similarities	Belongs to the psbQ family.
Sequence caution	The sequence CAB40384.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Membrane Photosystem II Plastid Thylakoid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast stroma Inferred from direct assay PubMed 16207701PubMed 18633119. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay PubMed 14729914PubMed 15322131. Source: TAIR extrinsic to membrane Inferred from electronic annotation. Source: InterPro oxygen evolving complex Inferred from electronic annotation. Source: InterPro thylakoid lumen Inferred from direct assay PubMed 11719511. Source: TAIR
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9XFT3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Derived from EST data. No experimental confirmation available.

Isoform 2 (identifier: Q9XFT3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     94-94: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 44	44	Chloroplast Potential
	Transit peptide	45 – 77	33	Thylakoid Ref.6
	Chain	78 – 224	147	Oxygen-evolving enhancer protein 3-1, chloroplastic		PRO_0000029589
Natural variations
	Alternative sequence	94	1	Missing in isoform 2.		VSP_034388

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 2008. Version 3. Checksum: 3FBD68B52BB40462	FASTA	224	23,866
	10 20 30 40 50 60 MASMGGLHGA SPAVLEGSLK INGSSRLNGS GRVAVAQRSR LVVRAQQSEE TSRRSVIGLV 70 80 90 100 110 120 AAGLAGGSFV QAVLADAISI KVGPPPAPSG GLPAGTDNSD QARDFALALK DRFYLQPLPP 130 140 150 160 170 180 TEAAARAKES AKDIINVKPL IDRKAWPYVQ NDLRSKASYL RYDLNTIISS KPKDEKKSLK 190 200 210 220 DLTTKLFDTI DNLDYAAKKK SPSQAEKYYA ETVSALNEVL AKLG
	Isoform 2 [UniParc]. Checksum: 8ECA0513BE45F6CD	FASTA	223	23,795
	10 20 30 40 50 60 MASMGGLHGA SPAVLEGSLK INGSSRLNGS GRVAVAQRSR LVVRAQQSEE TSRRSVIGLV 70 80 90 100 110 120 AAGLAGGSFV QAVLADAISI KVGPPPAPSG GLPGTDNSDQ ARDFALALKD RFYLQPLPPT 130 140 150 160 170 180 EAAARAKESA KDIINVKPLI DRKAWPYVQN DLRSKASYLR YDLNTIISSK PKDEKKSLKD 190 200 210 220 LTTKLFDTID NLDYAAKKKS PSQAEKYYAE TVSALNEVLA KLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of psbQ gene for 16-kDa protein of oxygen-evolving complex from Arabidopsis thaliana and regulation of its expression." Grover M., Gaur T., Kochhar A., Maheshwari S.C., Tyagi A.K. DNA Res. 6:173-177(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION BY LIGHT. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]	"Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction." Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G., Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G., van Wijk K.J. Plant Cell 14:211-236(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Analysis of Arabidopsis PsbQA gene expression in transgenic tobacco reveals differential role of its promoter and transcribed region in organ-specific and light-mediated regulation." Gaur T., Tyagi A.K. Transgenic Res. 13:97-108(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION BY LIGHT.
[8]	"The PsbQ protein is required in Arabidopsis for photosystem II assembly/stability and photoautotrophy under low light conditions." Yi X., Hargett S.R., Frankel L.K., Bricker T.M. J. Biol. Chem. 281:26260-26267(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y16847 Genomic DNA. Translation: CAB40384.1. Sequence problems. AL021960 Genomic DNA. Translation: CAA17547.1. AL031187 Genomic DNA. Translation: CAA20194.1. AL161554 Genomic DNA. Translation: CAB79128.1. CP002687 Genomic DNA. Translation: AEE84435.1. CP002687 Genomic DNA. Translation: AEE84436.1. AY050328 mRNA. Translation: AAK91345.1. AY094048 mRNA. Translation: AAM16204.1. AY088330 mRNA. Translation: AAM65869.1.
IPI	IPI00532582. IPI00657124.
PIR	T04959.
RefSeq	NP_001031687.1. NM_001036610.1. NP_193860.1. NM_118247.2.
UniGene	At.24610.
3D structure databases
ProteinModelPortal	Q9XFT3.
SMR	Q9XFT3. Positions 76-224.
ModBase	Search...
2D gel databases
SWISS-2DPAGE	Q9XFT3.
Proteomic databases
PaxDb	Q9XFT3.
PRIDE	Q9XFT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G21280.2; AT4G21280.2; AT4G21280.
GeneID	827877.
KEGG	ath:AT4G21280.
Organism-specific databases
TAIR	At4g21280.
Phylogenomic databases
eggNOG	NOG313473.
HOGENOM	HOG000029167.
InParanoid	Q9XFT3.
KO	K08901.
OMA	DRFYLQP.
PhylomeDB	Q9XFT3.
ProtClustDB	CLSN2685363.
Enzyme and pathway databases
BioCyc	MetaCyc:AT4G21280-MONOMER.
Gene expression databases
Genevestigator	Q9XFT3.
Family and domain databases
Gene3D	1.20.120.290. 1 hit.
InterPro	IPR023222. PsbQ-like_domain. IPR008797. PSII_PsbQ. [Graphical view]
Pfam	PF05757. PsbQ. 1 hit. [Graphical view]
SUPFAM	SSF101112. PsbQ-like_domain. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PSBQ1_ARATH

Accession

Primary (citable) accession number: Q9XFT3
Secondary accession number(s): O49568, Q2V3G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program