Reviewed, UniProtKB/Swiss-Prot Q9XFS9 (DXR_ARATH)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic
      Short name=1-deoxyxylulose-5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	DXR
Ordered Locus Names:	At5g62790
ORF Names:	MQB2.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH.
Cofactor	Divalent cation By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Ref.1 Inferred from direct assay. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Ref.1 Inferred from direct assay. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 477		1-deoxy-D-xylulose 5-phosphate reductoisomerase, chloroplastic		PRO_0000007395
Regions
	Nucleotide binding	86 – 115	30	NADP By similarity
Sites
	Metal binding	229	1	Divalent metal cation By similarity
	Metal binding	231	1	Divalent metal cation By similarity
	Metal binding	300	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XFS9-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: C06A455AB73ACA7C	FASTA	477	51,964
	10 20 30 40 50 60 MMTLNSLSPA ESKAISFLDT SRFNPIPKLS GGFSLRRRNQ GRGFGKGVKC SVKVQQQQQP 70 80 90 100 110 120 PPAWPGRAVP EAPRQSWDGP KPISIVGSTG SIGTQTLDIV AENPDKFRVV ALAAGSNVTL 130 140 150 160 170 180 LADQVRRFKP ALVAVRNESL INELKEALAD LDYKLEIIPG EQGVIEVARH PEAVTVVTGI 190 200 210 220 230 240 VGCAGLKPTV AAIEAGKDIA LANKETLIAG GPFVLPLANK HNVKILPADS EHSAIFQCIQ 250 260 270 280 290 300 GLPEGALRKI ILTASGGAFR DWPVEKLKEV KVADALKHPN WNMGKKITVD SATLFNKGLE 310 320 330 340 350 360 VIEAHYLFGA EYDDIEIVIH PQSIIHSMIE TQDSSVLAQL GWPDMRLPIL YTMSWPDRVP 370 380 390 400 410 420 CSEVTWPRLD LCKLGSLTFK KPDNVKYPSM DLAYAAGRAG GTMTGVLSAA NEKAVEMFID 430 440 450 460 470 EKISYLDIFK VVELTCDKHR NELVTSPSLE EIVHYDLWAR EYAANVQLSS GARPVHA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the first committed enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway." Carretero-Paulet L., Ahumada I., Cunillera N., Rodriguez-Concepcion M., Ferrer A., Boronat A., Campos N. Plant Physiol. 129:1581-1591(2002) [PubMed: 12177470] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning and heterologous expression of a cDNA encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana." Schwender J., Mueller C., Zeidler J., Lichtenthaler H.K. FEBS Lett. 455:140-144(1999) [PubMed: 10428488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 72-477. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF148852 mRNA. Translation: AAF73140.1. AB009053 Genomic DNA. Translation: BAB10848.1. AY045634 mRNA. Translation: AAK73992.1. AY050802 mRNA. Translation: AAK92737.1. AY054682 mRNA. Translation: AAK96873.1. AY081453 mRNA. Translation: AAM10015.1. AY091405 mRNA. Translation: AAM14344.1. AY098952 mRNA. Translation: AAM19962.1. AY084775 mRNA. Translation: AAM61343.1. AJ242588 mRNA. Translation: CAB43344.1.
IPI	IPI00519417.
PIR	T52570.
RefSeq	NP_201085.1.
UniGene	At.23021
3D structure databases
HSSP	HSSP built from PDB template 1K5H based on UniProtKB P45568.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9XFS9.
Proteomic databases
PRIDE	Q9XFS9.
Genome annotation databases
GeneID	836400.
GenomeReviews	Gene locus AT5G62790 in contig BA000015_GR.
KEGG	ath:AT5G62790.
NMPDR	fig|3702.1.peg.28321.
Organism-specific databases
TAIR	At5g62790.
Phylogenomic databases
OMA	IHSMVEY.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G62790-MON.
BRENDA	1.1.1.267. 302.
Gene expression databases
ArrayExpress	Q9XFS9.
Genevestigator	Q9XFS9.
GermOnline	AT5G62790. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_ARATH

Accession

Primary (citable) accession number: Q9XFS9
Secondary accession number(s): Q9M6U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)