Q9XFM4 (13S3_FAGES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
13S globulin seed storage protein 3

Alternative name(s):
Legumin-like protein 3
Allergen=Fag e 1

Cleaved into the following 2 chains:

1. 13S globulin seed storage protein 3 acidic chain
2. 13S globulin seed storage protein 3 basic chain

Gene names

Name:

FAGAG1

Organism

Fagopyrum esculentum (Common buckwheat) (Polygonum fagopyrum)

Taxonomic identifier

3617 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Polygonaceae › Polygonoideae › Fagopyreae › Fagopyrum

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seed storage protein.
Subunit structure	Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond By similarity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the 11S seed storage protein (globulins) family.

Ontologies

Keywords
Disease	Allergen
Domain	Signal
Molecular function	Seed storage protein Storage protein
PTM	Disulfide bond
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 347	327	13S globulin seed storage protein 3 acidic chain By similarity		PRO_0000032007
	Chain	348 – 538	191	13S globulin seed storage protein 3 basic chain By similarity		PRO_0000032008
Amino acid modifications
	Disulfide bond	44 ↔ 77		By similarity
	Disulfide bond	120 ↔ 354		Interchain (between acidic and basic chains) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9XFM4 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 41D6BA55220CFDAC	FASTA	538	61,164
	10 20 30 40 50 60 MSTKLILSFS LCLMVLSCSA QLLPWQKGQR SRPHHGHQQF QHQCDIQRLT ASEPSRRVRS 70 80 90 100 110 120 EAGVTEIWDH DTPEFRCAGF VAVRVVIQPG GLLLPSYSNA PYITFVEQGR GVQGVVVPGC 130 140 150 160 170 180 PETFQSGSEF EYPRSQRDQR SRQSESGESS RGDQRSRQSE SEESSRGDQR SRQSESEEFS 190 200 210 220 230 240 RGDQHQKIFR IRDGDVIPSP AGVVQWTHNN GDNDLISITL YDANSFQNQL DENVRNFFLA 250 260 270 280 290 300 GQSKQSREDR RSQRQTREEG SDRQSRESQD DEALLEANIL SGFEDEILQE IFRNVDQETI 310 320 330 340 350 360 SKLRGENDQR GFIVQARDLK LRVPEEYEEE LQRERGDRKR GGSGRSNGLE QAFCNLKFRQ 370 380 390 400 410 420 NVNRPSRADV FNPRAGRINT VDSNNLPILE FIQLSAQHVV LYKNAILGPR WNLNAHSALY 430 440 450 460 470 480 VTRGEGRVQV VGDEGRSVFD DNVQRGQILV VPQGFAVVLK AGREGLEWVE LKNDDNAITS 490 500 510 520 530 PIAGKTSVLR AIPVEVLANS YDISTKEAFR LKNGRQEVEV FRPFQSRDEK ERERFSIV

References

[1]	"A molecular-genetic approach for hypoallergenic buckwheat." Nair A., Ohmoto T., Woo S.H., Adachi T. Fagopyrum 16:29-36(1999) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Miyazaki zairai.
[2]	"Screening and selection of hypoallergenic buckwheat species." Nair A., Adachi T. ScientificWorldJournal 2:818-826(2002) [PubMed: 12806007] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 348-538.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF152003 mRNA. Translation: AAD32713.1. AF216801 Genomic DNA. Translation: AAF34635.1.
3D structure databases
ProteinModelPortal	Q9XFM4.
ModBase	Search...
Protein family/group databases
Allergome	698. Fag e 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006044. 11S_seedstore_pln. IPR006045. Cupin_1. IPR011051. Cupin_RmlC_type. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 3 hits.
Pfam	PF00190. Cupin_1. 2 hits. [Graphical view]
PRINTS	PR00439. 11SGLOBULIN.
SMART	SM00835. Cupin_1. 2 hits. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

13S3_FAGES

Accession

Primary (citable) accession number: Q9XFM4
Secondary accession number(s): Q9M641

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program