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Q9XFM0 (IAA28_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Auxin-responsive protein IAA28
Alternative name(s):
Indoleacetic acid-induced protein 28
Gene names
Name:IAA28
Ordered Locus Names:At5g25890
ORF Names:F18A17.6, T1N24.24
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression. Ref.5

Subunit structure

Homodimers and heterodimers By similarity. Interacts with TPL. Ref.7

Subcellular location

Nucleus By similarity.

Tissue specificity

In roots and inflorescence stems.

Induction

Not induced by auxin.

Domain

The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV. Ref.6

Sequence similarities

Belongs to the Aux/IAA family.

Contains 1 Aux/IAA-ARF domain.

Sequence caution

The sequence AAD40120.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 175175Auxin-responsive protein IAA28
PRO_0000112854

Regions

Domain81 – 16282Aux/IAA-ARF
Motif7 – 115EAR-like (transcriptional repression)

Experimental info

Mutagenesis531P → L in iaa28-1; gain of function. Affects auxin-related developmental processes.

Sequences

Sequence LengthMass (Da)Tools
Q9XFM0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 7A58B615DBC8FB47

FASTA17520,206
        10         20         30         40         50         60 
MEEEKRLELR LAPPCHQFTS NNNINGSKQK SSTKETSFLS NNRVEVAPVV GWPPVRSSRR 

        70         80         90        100        110        120 
NLTAQLKEEM KKKESDEEKE LYVKINMEGV PIGRKVNLSA YNNYQQLSHA VDQLFSKKDS 

       130        140        150        160        170 
WDLNRQYTLV YEDTEGDKVL VGDVPWEMFV STVKRLHVLK TSHAFSLSPR KHGKE 

« Hide

References

« Hide 'large scale' references
[1]"A gain-of-function mutation in IAA28 suppresses lateral root development."
Rogg L.E., Lasswell J.E., Bartel B.
Plant Cell 13:465-480(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTANT IAA28-1.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Genetics of Aux/IAA and ARF action in plant growth and development."
Liscum E., Reed J.W.
Plant Mol. Biol. 49:387-400(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[6]"Aux/IAA proteins contain a potent transcriptional repression domain."
Tiwari S.B., Hagen G., Guilfoyle T.J.
Plant Cell 16:533-543(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TRANSCRIPTIONAL REPRESSION DOMAIN.
[7]"TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis."
Szemenyei H., Hannon M., Long J.A.
Science 319:1384-1386(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TPL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF149816 mRNA. Translation: AAD34019.1.
AC005405 Genomic DNA. No translation available.
AF149413 Genomic DNA. Translation: AAD40120.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93498.1.
BT000427 mRNA. Translation: AAN17404.1.
BT002175 mRNA. Translation: AAN72186.1.
PIRT52143.
RefSeqNP_568478.1. NM_122490.3.
UniGeneAt.11439.

3D structure databases

ProteinModelPortalQ9XFM0.
SMRQ9XFM0. Positions 81-167.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid17933. 14 interactions.
IntActQ9XFM0. 40 interactions.
STRING3702.AT5G25890.1-P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G25890.1; AT5G25890.1; AT5G25890.
GeneID832658.
KEGGath:AT5G25890.

Organism-specific databases

GeneFarm3188. 342.
TAIRAT5G25890.

Phylogenomic databases

eggNOGNOG283222.
HOGENOMHOG000238261.
InParanoidQ9XFM0.
KOK14484.
OMAWEMFVST.
PhylomeDBQ9XFM0.

Gene expression databases

GenevestigatorQ9XFM0.

Family and domain databases

InterProIPR003311. AUX_IAA.
IPR011525. AuxIAA_ARF_dimer.
[Graphical view]
PfamPF02309. AUX_IAA. 1 hit.
[Graphical view]
PROSITEPS50962. IAA_ARF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIAA28_ARATH
AccessionPrimary (citable) accession number: Q9XFM0
Secondary accession number(s): Q9S9V6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names