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Q9XFM0

- IAA28_ARATH

UniProt

Q9XFM0 - IAA28_ARATH

Protein

Auxin-responsive protein IAA28

Gene

IAA28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. auxin-activated signaling pathway Source: UniProtKB-KW
    2. lateral root morphogenesis Source: TAIR
    3. response to auxin Source: TAIR
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Auxin signaling pathway, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Auxin-responsive protein IAA28
    Alternative name(s):
    Indoleacetic acid-induced protein 28
    Gene namesi
    Name:IAA28
    Ordered Locus Names:At5g25890
    ORF Names:F18A17.6, T1N24.24
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G25890.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi53 – 531P → L in iaa28-1; gain of function. Affects auxin-related developmental processes.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 175175Auxin-responsive protein IAA28PRO_0000112854Add
    BLAST

    Expressioni

    Tissue specificityi

    In roots and inflorescence stems.

    Inductioni

    Not induced by auxin.

    Gene expression databases

    GenevestigatoriQ9XFM0.

    Interactioni

    Subunit structurei

    Homodimers and heterodimers By similarity. Interacts with TPL.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARF6Q9ZTX83EBI-3133404,EBI-3946770
    ARF9Q9XED83EBI-3133404,EBI-3946762
    IAA11Q388293EBI-3133404,EBI-2367923

    Protein-protein interaction databases

    BioGridi17933. 14 interactions.
    IntActiQ9XFM0. 40 interactions.
    STRINGi3702.AT5G25890.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9XFM0.
    SMRiQ9XFM0. Positions 81-167.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 16282Aux/IAA-ARFPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi7 – 115EAR-like (transcriptional repression)

    Domaini

    The N-terminal half of the protein contains two conserved domains I and II. Domain I includes a slightly degenerated ERF-associated amphiphilic repression (EAR) motif which seems to be involved in the activity of transcriptional repression. Domain II is required for the correct degradation of the protein through the SCF-mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA proteins and auxin response factors (ARFs) occur through their C-terminal dimerization domains III and IV.

    Sequence similaritiesi

    Belongs to the Aux/IAA family.Curated
    Contains 1 Aux/IAA-ARF domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG283222.
    HOGENOMiHOG000238261.
    InParanoidiQ9XFM0.
    KOiK14484.
    OMAiWEMFVST.
    PhylomeDBiQ9XFM0.

    Family and domain databases

    InterProiIPR003311. AUX_IAA.
    IPR011525. AuxIAA_ARF_dimer.
    [Graphical view]
    PfamiPF02309. AUX_IAA. 1 hit.
    [Graphical view]
    PROSITEiPS50962. IAA_ARF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9XFM0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEEKRLELR LAPPCHQFTS NNNINGSKQK SSTKETSFLS NNRVEVAPVV    50
    GWPPVRSSRR NLTAQLKEEM KKKESDEEKE LYVKINMEGV PIGRKVNLSA 100
    YNNYQQLSHA VDQLFSKKDS WDLNRQYTLV YEDTEGDKVL VGDVPWEMFV 150
    STVKRLHVLK TSHAFSLSPR KHGKE 175
    Length:175
    Mass (Da):20,206
    Last modified:November 1, 1999 - v1
    Checksum:i7A58B615DBC8FB47
    GO

    Sequence cautioni

    The sequence AAD40120.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF149816 mRNA. Translation: AAD34019.1.
    AC005405 Genomic DNA. No translation available.
    AF149413 Genomic DNA. Translation: AAD40120.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED93498.1.
    BT000427 mRNA. Translation: AAN17404.1.
    BT002175 mRNA. Translation: AAN72186.1.
    PIRiT52143.
    RefSeqiNP_568478.1. NM_122490.3.
    UniGeneiAt.11439.

    Genome annotation databases

    EnsemblPlantsiAT5G25890.1; AT5G25890.1; AT5G25890.
    GeneIDi832658.
    KEGGiath:AT5G25890.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF149816 mRNA. Translation: AAD34019.1 .
    AC005405 Genomic DNA. No translation available.
    AF149413 Genomic DNA. Translation: AAD40120.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED93498.1 .
    BT000427 mRNA. Translation: AAN17404.1 .
    BT002175 mRNA. Translation: AAN72186.1 .
    PIRi T52143.
    RefSeqi NP_568478.1. NM_122490.3.
    UniGenei At.11439.

    3D structure databases

    ProteinModelPortali Q9XFM0.
    SMRi Q9XFM0. Positions 81-167.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 17933. 14 interactions.
    IntActi Q9XFM0. 40 interactions.
    STRINGi 3702.AT5G25890.1-P.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G25890.1 ; AT5G25890.1 ; AT5G25890 .
    GeneIDi 832658.
    KEGGi ath:AT5G25890.

    Organism-specific databases

    GeneFarmi 3188. 342.
    TAIRi AT5G25890.

    Phylogenomic databases

    eggNOGi NOG283222.
    HOGENOMi HOG000238261.
    InParanoidi Q9XFM0.
    KOi K14484.
    OMAi WEMFVST.
    PhylomeDBi Q9XFM0.

    Gene expression databases

    Genevestigatori Q9XFM0.

    Family and domain databases

    InterProi IPR003311. AUX_IAA.
    IPR011525. AuxIAA_ARF_dimer.
    [Graphical view ]
    Pfami PF02309. AUX_IAA. 1 hit.
    [Graphical view ]
    PROSITEi PS50962. IAA_ARF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A gain-of-function mutation in IAA28 suppresses lateral root development."
      Rogg L.E., Lasswell J.E., Bartel B.
      Plant Cell 13:465-480(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTANT IAA28-1.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Genetics of Aux/IAA and ARF action in plant growth and development."
      Liscum E., Reed J.W.
      Plant Mol. Biol. 49:387-400(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
    6. "Aux/IAA proteins contain a potent transcriptional repression domain."
      Tiwari S.B., Hagen G., Guilfoyle T.J.
      Plant Cell 16:533-543(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TRANSCRIPTIONAL REPRESSION DOMAIN.
    7. "TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis."
      Szemenyei H., Hannon M., Long J.A.
      Science 319:1384-1386(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TPL.

    Entry informationi

    Entry nameiIAA28_ARATH
    AccessioniPrimary (citable) accession number: Q9XFM0
    Secondary accession number(s): Q9S9V6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3