Q9XF98 (CALR_PRUAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Calreticulin
Organism	Prunus armeniaca (Apricot)
Taxonomic identifier	36596 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Amygdaloideae › Amygdaleae › Prunus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Domain	Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity. The interaction with glycans occurs through a binding site in the globular lectin domain By similarity. The zinc binding sites are localized to the N-domain By similarity.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Lectin Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 421	399	Calreticulin		PRO_0000004194
Regions
	Repeat	196 – 207	12	1-1
	Repeat	215 – 226	12	1-2
	Repeat	232 – 243	12	1-3
	Repeat	250 – 261	12	1-4
	Repeat	265 – 275	11	2-1
	Repeat	279 – 289	11	2-2
	Repeat	293 – 303	11	2-3
	Region	196 – 261	66	4 X approximate repeats
	Region	265 – 303	39	3 X approximate repeats
	Motif	418 – 421	4	Prevents secretion from ER Potential
	Compositional bias	357 – 417	61	Asp/Glu/Lys-rich
Sites
	Binding site	114	1	Carbohydrate By similarity
	Binding site	116	1	Carbohydrate By similarity
	Binding site	133	1	Carbohydrate By similarity
	Binding site	140	1	Carbohydrate By similarity
	Binding site	323	1	Carbohydrate By similarity
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	156	1	N-linked (GlcNAc...) Potential
	Disulfide bond	110 ↔ 142		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XF98 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 4F5F94CBAA6C6690	FASTA	421	48,416
	10 20 30 40 50 60 MAFRVPNSSL LSLILLSLLA IASAKVFFEE RFEDGWDKRW VTSEWKKDEN LAGEWNYTSG 70 80 90 100 110 120 KWNGDPNDKG IQTSEDYRFY AISAEFPEFS NKDKTLVFQF SVKHEQKLDC GGGYIKLLSG 130 140 150 160 170 180 DVDQKKFGGD TPYSIMFGPD ICGYSTKKVH AILNYNNTNN LIKKDVPCET DQLTHVYTFI 190 200 210 220 230 240 IRPDATYSIL IDNLEKQTGS LYSDWDLLPA KKIKDPEAKK PEDWEDQEYI PDPEDKKPEG 250 260 270 280 290 300 YDDIPKEITD PDAKKPEDWD DEEDGEWTAP TIPNPEYKGE WKPKKIKNPN FKGKWKAPLI 310 320 330 340 350 360 DNPEFKDDPE LYVYPNLKYV GIELWQVKSG TLFDNILITD EPEYAKQLAE ETWGKQKDAE 370 380 390 400 410 420 KAAFEELEKK LQEEESKEDP VDSDAEDDDN EAEDGEESDS ESKPDSTEES AETEAEKHDE L

References

[1]	"Molecular cloning and nucleotide sequence of a calreticulin from apricot (Prunus armeniaca cv. Bergeron)." Mbeguie-A-Mbeguie D., Fils-Lycaon B.R. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bergeron. Tissue: Endocarp and Mesocarp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF134733 mRNA. Translation: AAD32207.1.
3D structure databases
ProteinModelPortal	Q9XF98.
SMR	Q9XF98. Positions 210-311.
ModBase	Search...
Proteomic databases
PRIDE	Q9XF98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.10.250.10. 1 hit. 2.60.120.200. 1 hit.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR009033. Calreticulin/calnexin_P_dom. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF63887. Calret_calnex_P. 1 hit. SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00805. CALRETICULIN_REPEAT. 2 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALR_PRUAR

Accession

Primary (citable) accession number: Q9XF98

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	March 6, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program