Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9XF98 (CALR_PRUAR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calreticulin
OrganismPrunus armeniaca (Apricot)
Taxonomic identifier36596 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeAmygdaleaePrunus

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.

Subcellular location

Endoplasmic reticulum lumen By similarity.

Domain

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.

The interaction with glycans occurs through a binding site in the globular lectin domain By similarity.

The zinc binding sites are localized to the N-domain By similarity.

Sequence similarities

Belongs to the calreticulin family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
   DomainRepeat
Signal
   LigandCalcium
Lectin
Metal-binding
Zinc
   Molecular functionChaperone
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

   Cellular_componentendoplasmic reticulum lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 421399Calreticulin
PRO_0000004194

Regions

Repeat196 – 207121-1
Repeat215 – 226121-2
Repeat232 – 243121-3
Repeat250 – 261121-4
Repeat265 – 275112-1
Repeat279 – 289112-2
Repeat293 – 303112-3
Region196 – 261664 X approximate repeats
Region265 – 303393 X approximate repeats
Motif418 – 4214Prevents secretion from ER Potential
Compositional bias357 – 41761Asp/Glu/Lys-rich

Sites

Binding site1141Carbohydrate By similarity
Binding site1161Carbohydrate By similarity
Binding site1331Carbohydrate By similarity
Binding site1401Carbohydrate By similarity
Binding site3231Carbohydrate By similarity

Amino acid modifications

Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation1561N-linked (GlcNAc...) Potential
Disulfide bond110 ↔ 142 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9XF98 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 4F5F94CBAA6C6690

FASTA42148,416
        10         20         30         40         50         60 
MAFRVPNSSL LSLILLSLLA IASAKVFFEE RFEDGWDKRW VTSEWKKDEN LAGEWNYTSG 

        70         80         90        100        110        120 
KWNGDPNDKG IQTSEDYRFY AISAEFPEFS NKDKTLVFQF SVKHEQKLDC GGGYIKLLSG 

       130        140        150        160        170        180 
DVDQKKFGGD TPYSIMFGPD ICGYSTKKVH AILNYNNTNN LIKKDVPCET DQLTHVYTFI 

       190        200        210        220        230        240 
IRPDATYSIL IDNLEKQTGS LYSDWDLLPA KKIKDPEAKK PEDWEDQEYI PDPEDKKPEG 

       250        260        270        280        290        300 
YDDIPKEITD PDAKKPEDWD DEEDGEWTAP TIPNPEYKGE WKPKKIKNPN FKGKWKAPLI 

       310        320        330        340        350        360 
DNPEFKDDPE LYVYPNLKYV GIELWQVKSG TLFDNILITD EPEYAKQLAE ETWGKQKDAE 

       370        380        390        400        410        420 
KAAFEELEKK LQEEESKEDP VDSDAEDDDN EAEDGEESDS ESKPDSTEES AETEAEKHDE 


L 

« Hide

References

[1]"Molecular cloning and nucleotide sequence of a calreticulin from apricot (Prunus armeniaca cv. Bergeron)."
Mbeguie-A-Mbeguie D., Fils-Lycaon B.R.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Bergeron.
Tissue: Endocarp and Mesocarp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF134733 mRNA. Translation: AAD32207.1.

3D structure databases

ProteinModelPortalQ9XF98.
SMRQ9XF98. Positions 210-311.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9XF98.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PANTHERPTHR11073. PTHR11073. 1 hit.
PfamPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFPIRSF002356. Calreticulin. 1 hit.
PRINTSPR00626. CALRETICULIN.
SUPFAMSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALR_PRUAR
AccessionPrimary (citable) accession number: Q9XF98
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families