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Protein

3-ketoacyl-CoA synthase 6

Gene

CUT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to cuticular wax and suberin biosynthesis. Involved in both decarbonylation and acyl-reduction wax synthesis pathways. Required for elongation of C24 fatty acids, an essential step of the cuticular wax production (PubMed:10330468, PubMed:11041893). Major condensing enzyme for stem wax and pollen coat lipid biosynthesis (PubMed:12467640).3 Publications

Caution

Catalytic activityi

A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO2.1 Publication

Enzyme regulationi

Strongly inhibited by metazachlor and mefluidide.1 Publication

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei225By similarity1
Active sitei304By similarity1
Active sitei388By similarity1
Active sitei392By similarity1
Active sitei421By similarity1
Active sitei425By similarity1

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-UniPathway
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
  • unidimensional cell growth Source: TAIR
  • wax biosynthetic process Source: TAIR

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G68530-MONOMER
MetaCyc:AT1G68530-MONOMER
BRENDAi2.3.1.199 399
UniPathwayiUPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA synthase 61 Publication (EC:2.3.1.1991 Publication)
Short name:
KCS-61 Publication
Alternative name(s):
Cuticular protein 11 Publication
Eceriferum 61 Publication
Very long-chain fatty acid condensing enzyme 61 Publication
Short name:
VLCFA condensing enzyme 61 Publication
Gene namesi
Name:CUT11 Publication
Synonyms:CER61 Publication, EL6, KCS61 Publication
Ordered Locus Names:At1g68530Imported
ORF Names:T26J14.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G68530
TAIRilocus:2201262 AT1G68530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants have no wax crystals and are male sterile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002490981 – 4973-ketoacyl-CoA synthase 6Add BLAST497

Proteomic databases

PaxDbiQ9XF43
PRIDEiQ9XF43

Expressioni

Tissue specificityi

In epidermal cells of aerial tissues and in the tapetum of anthers near maturity (PubMed:10330468, PubMed:12177469). Expressed in siliques, flowers and leaves (PubMed:18465198).3 Publications

Inductioni

Repressed by herbicides such as flufenacet and benfuresate (PubMed:12916765). Up-regulated by osmotic stress and abscisic acid and down-regulated by darkness (PubMed:12177469, PubMed:18465198). Down-regulated by low temperature and up-regulated by salt and drought (PubMed:18465198).3 Publications

Gene expression databases

ExpressionAtlasiQ9XF43 baseline and differential
GenevisibleiQ9XF43 AT

Interactioni

Protein-protein interaction databases

BioGridi284031 interactor.
STRINGi3702.AT1G68530.1

Structurei

3D structure databases

ProteinModelPortaliQ9XF43
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 370FAESequence analysisAdd BLAST290

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHMW Eukaryota
ENOG410Y5VH LUCA
HOGENOMiHOG000238893
InParanoidiQ9XF43
KOiK15397
OMAiFYCVYEQ
OrthoDBiEOG093606UZ
PhylomeDBiQ9XF43

Family and domain databases

Gene3Di3.40.47.101 hit
InterProiView protein in InterPro
IPR012392 3-ktacl-CoA_syn
IPR013747 ACP_syn_III_C
IPR013601 FAE1_typ3_polyketide_synth
IPR016039 Thiolase-like
PANTHERiPTHR31561 PTHR31561, 1 hit
PfamiView protein in Pfam
PF08541 ACP_syn_III_C, 1 hit
PF08392 FAE1_CUT1_RppA, 1 hit
PIRSFiPIRSF036417 3-ktacl-CoA_syn, 1 hit
SUPFAMiSSF53901 SSF53901, 3 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9XF43-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQAPMPEFS SSVKLKYVKL GYQYLVNHFL SFLLIPIMAI VAVELLRMGP
60 70 80 90 100
EEILNVWNSL QFDLVQVLCS SFFVIFISTV YFMSKPRTIY LVDYSCYKPP
110 120 130 140 150
VTCRVPFATF MEHSRLILKD KPKSVEFQMR ILERSGLGEE TCLPPAIHYI
160 170 180 190 200
PPTPTMDAAR SEAQMVIFEA MDDLFKKTGL KPKDVDILIV NCSLFSPTPS
210 220 230 240 250
LSAMVINKYK LRSNIKSFNL SGMGCSAGLI SVDLARDLLQ VHPNSNAIIV
260 270 280 290 300
STEIITPNYY QGNERAMLLP NCLFRMGAAA IHMSNRRSDR WRAKYKLSHL
310 320 330 340 350
VRTHRGADDK SFYCVYEQED KEGHVGINLS KDLMAIAGEA LKANITTIGP
360 370 380 390 400
LVLPASEQLL FLTSLIGRKI FNPKWKPYIP DFKLAFEHFC IHAGGRAVID
410 420 430 440 450
ELQKNLQLSG EHVEASRMTL HRFGNTSSSS LWYELSYIES KGRMRRGDRV
460 470 480 490
WQIAFGSGFK CNSAVWKCNR TIKTPKDGPW SDCIDRYPVF IPEVVKL
Length:497
Mass (Da):56,396
Last modified:November 1, 1999 - v1
Checksum:iB371C7948B33D6EB
GO
Isoform 2 (identifier: Q9XF43-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     350-377: PLVLPASEQLLFLTSLIGRKIFNPKWKP → NKHTSFYFTYIYTLTMIYTVKTISRGGH
     378-497: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:377
Mass (Da):42,723
Checksum:i4066831ED82FFB41
GO

Sequence cautioni

The sequence BAD94789 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73F → L in AAL50069 (PubMed:14593172).Curated1
Sequence conflicti73F → L in AAM16230 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022340350 – 377PLVLP…PKWKP → NKHTSFYFTYIYTLTMIYTV KTISRGGH in isoform 2. CuratedAdd BLAST28
Alternative sequenceiVSP_022341378 – 497Missing in isoform 2. CuratedAdd BLAST120

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129511 mRNA Translation: AAD37122.1
AC011915 Genomic DNA Translation: AAG52390.1
CP002684 Genomic DNA Translation: AEE34804.1
CP002684 Genomic DNA Translation: AEE34805.1
AY070727 mRNA Translation: AAL50069.1
AY093969 mRNA Translation: AAM16230.1
AK221042 mRNA Translation: BAD94789.1 Different initiation.
PIRiT52308
RefSeqiNP_177020.1, NM_105524.3 [Q9XF43-1]
NP_849861.1, NM_179530.1 [Q9XF43-2]
UniGeneiAt.23200
At.66994

Genome annotation databases

EnsemblPlantsiAT1G68530.1; AT1G68530.1; AT1G68530 [Q9XF43-1]
AT1G68530.2; AT1G68530.2; AT1G68530 [Q9XF43-2]
GeneIDi843182
GrameneiAT1G68530.1; AT1G68530.1; AT1G68530 [Q9XF43-1]
AT1G68530.2; AT1G68530.2; AT1G68530 [Q9XF43-2]
KEGGiath:AT1G68530

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKCS6_ARATH
AccessioniPrimary (citable) accession number: Q9XF43
Secondary accession number(s): Q3ECG1, Q56ZC4, Q8VYJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1999
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome