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Protein

3-ketoacyl-CoA synthase 6

Gene

CUT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to cuticular wax and suberin biosynthesis. Involved in both decarbonylation and acyl-reduction wax synthesis pathways. Required for elongation of C24 fatty acids, an essential step of the cuticular wax production (PubMed:10330468, PubMed:11041893). Major condensing enzyme for stem wax and pollen coat lipid biosynthesis (PubMed:12467640).3 Publications

Catalytic activityi

A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO2.1 Publication

Enzyme regulationi

Strongly inhibited by metazachlor and mefluidide.1 Publication

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei225 – 2251By similarity
Active sitei304 – 3041By similarity
Active sitei388 – 3881By similarity
Active sitei392 – 3921By similarity
Active sitei421 – 4211By similarity
Active sitei425 – 4251By similarity

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-UniPathway
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
  • unidimensional cell growth Source: TAIR
  • wax biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G68530-MONOMER.
ARA:GQT-325-MONOMER.
MetaCyc:AT1G68530-MONOMER.
BRENDAi2.3.1.199. 399.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA synthase 61 Publication (EC:2.3.1.1991 Publication)
Short name:
KCS-61 Publication
Alternative name(s):
Cuticular protein 11 Publication
Eceriferum 61 Publication
Very long-chain fatty acid condensing enzyme 61 Publication
Short name:
VLCFA condensing enzyme 61 Publication
Gene namesi
Name:CUT11 Publication
Synonyms:CER61 Publication, EL6, KCS61 Publication
Ordered Locus Names:At1g68530Imported
ORF Names:T26J14.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G68530.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei25 – 4521HelicalSequence analysisAdd
BLAST
Transmembranei64 – 8421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants have no wax crystals and are male sterile.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4974973-ketoacyl-CoA synthase 6PRO_0000249098Add
BLAST

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9XF43.
PRIDEiQ9XF43.

Expressioni

Tissue specificityi

In epidermal cells of aerial tissues and in the tapetum of anthers near maturity (PubMed:10330468, PubMed:12177469). Expressed in siliques, flowers and leaves (PubMed:18465198).3 Publications

Inductioni

Repressed by herbicides such as flufenacet and benfuresate (PubMed:12916765). Up-regulated by osmotic stress and abscisic acid and down-regulated by darkness (PubMed:12177469, PubMed:18465198). Down-regulated by low temperature and up-regulated by salt and drought (PubMed:18465198).3 Publications

Gene expression databases

GenevisibleiQ9XF43. AT.

Interactioni

Protein-protein interaction databases

BioGridi28403. 1 interaction.
STRINGi3702.AT1G68530.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XF43.
SMRiQ9XF43. Positions 159-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 370290FAESequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated
Contains 1 FAE (fatty acid elongase) domain.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHMW. Eukaryota.
ENOG410Y5VH. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ9XF43.
KOiK15397.
OMAiPTDGPWD.
OrthoDBiEOG093606UZ.
PhylomeDBiQ9XF43.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9XF43-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQAPMPEFS SSVKLKYVKL GYQYLVNHFL SFLLIPIMAI VAVELLRMGP
60 70 80 90 100
EEILNVWNSL QFDLVQVLCS SFFVIFISTV YFMSKPRTIY LVDYSCYKPP
110 120 130 140 150
VTCRVPFATF MEHSRLILKD KPKSVEFQMR ILERSGLGEE TCLPPAIHYI
160 170 180 190 200
PPTPTMDAAR SEAQMVIFEA MDDLFKKTGL KPKDVDILIV NCSLFSPTPS
210 220 230 240 250
LSAMVINKYK LRSNIKSFNL SGMGCSAGLI SVDLARDLLQ VHPNSNAIIV
260 270 280 290 300
STEIITPNYY QGNERAMLLP NCLFRMGAAA IHMSNRRSDR WRAKYKLSHL
310 320 330 340 350
VRTHRGADDK SFYCVYEQED KEGHVGINLS KDLMAIAGEA LKANITTIGP
360 370 380 390 400
LVLPASEQLL FLTSLIGRKI FNPKWKPYIP DFKLAFEHFC IHAGGRAVID
410 420 430 440 450
ELQKNLQLSG EHVEASRMTL HRFGNTSSSS LWYELSYIES KGRMRRGDRV
460 470 480 490
WQIAFGSGFK CNSAVWKCNR TIKTPKDGPW SDCIDRYPVF IPEVVKL
Length:497
Mass (Da):56,396
Last modified:November 1, 1999 - v1
Checksum:iB371C7948B33D6EB
GO
Isoform 2 (identifier: Q9XF43-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     350-377: PLVLPASEQLLFLTSLIGRKIFNPKWKP → NKHTSFYFTYIYTLTMIYTVKTISRGGH
     378-497: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:377
Mass (Da):42,723
Checksum:i4066831ED82FFB41
GO

Sequence cautioni

The sequence BAD94789 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731F → L in AAL50069 (PubMed:14593172).Curated
Sequence conflicti73 – 731F → L in AAM16230 (PubMed:14593172).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei350 – 37728PLVLP…PKWKP → NKHTSFYFTYIYTLTMIYTV KTISRGGH in isoform 2. CuratedVSP_022340Add
BLAST
Alternative sequencei378 – 497120Missing in isoform 2. CuratedVSP_022341Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129511 mRNA. Translation: AAD37122.1.
AC011915 Genomic DNA. Translation: AAG52390.1.
CP002684 Genomic DNA. Translation: AEE34804.1.
CP002684 Genomic DNA. Translation: AEE34805.1.
AY070727 mRNA. Translation: AAL50069.1.
AY093969 mRNA. Translation: AAM16230.1.
AK221042 mRNA. Translation: BAD94789.1. Different initiation.
PIRiT52308.
RefSeqiNP_177020.1. NM_105524.2. [Q9XF43-1]
NP_849861.1. NM_179530.1. [Q9XF43-2]
UniGeneiAt.23200.
At.66994.

Genome annotation databases

EnsemblPlantsiAT1G68530.1; AT1G68530.1; AT1G68530. [Q9XF43-1]
GeneIDi843182.
KEGGiath:AT1G68530.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129511 mRNA. Translation: AAD37122.1.
AC011915 Genomic DNA. Translation: AAG52390.1.
CP002684 Genomic DNA. Translation: AEE34804.1.
CP002684 Genomic DNA. Translation: AEE34805.1.
AY070727 mRNA. Translation: AAL50069.1.
AY093969 mRNA. Translation: AAM16230.1.
AK221042 mRNA. Translation: BAD94789.1. Different initiation.
PIRiT52308.
RefSeqiNP_177020.1. NM_105524.2. [Q9XF43-1]
NP_849861.1. NM_179530.1. [Q9XF43-2]
UniGeneiAt.23200.
At.66994.

3D structure databases

ProteinModelPortaliQ9XF43.
SMRiQ9XF43. Positions 159-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28403. 1 interaction.
STRINGi3702.AT1G68530.1.

Proteomic databases

PaxDbiQ9XF43.
PRIDEiQ9XF43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G68530.1; AT1G68530.1; AT1G68530. [Q9XF43-1]
GeneIDi843182.
KEGGiath:AT1G68530.

Organism-specific databases

TAIRiAT1G68530.

Phylogenomic databases

eggNOGiENOG410IHMW. Eukaryota.
ENOG410Y5VH. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ9XF43.
KOiK15397.
OMAiPTDGPWD.
OrthoDBiEOG093606UZ.
PhylomeDBiQ9XF43.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciARA:AT1G68530-MONOMER.
ARA:GQT-325-MONOMER.
MetaCyc:AT1G68530-MONOMER.
BRENDAi2.3.1.199. 399.

Miscellaneous databases

PROiQ9XF43.

Gene expression databases

GenevisibleiQ9XF43. AT.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiKCS6_ARATH
AccessioniPrimary (citable) accession number: Q9XF43
Secondary accession number(s): Q3ECG1, Q56ZC4, Q8VYJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.