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Protein

Peroxiredoxin-2B

Gene

PRXIIB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin or glutaredoxin system. May be involved in intracellular redox signaling.1 Publication
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule. C(P) is reactivated by glutaredoxin (Grx).1 Publication

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei51Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciARA:AT1G65980-MONOMER.

Protein family/group databases

PeroxiBasei4344. AtPrxII02.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-2B (EC:1.11.1.15)
Alternative name(s):
Peroxiredoxin IIB
Peroxiredoxin TPx1
Thioredoxin peroxidase 2B
Thioredoxin-dependent peroxidase 1
Gene namesi
Name:PRXIIB
Synonyms:TPX1
Ordered Locus Names:At1g65980
ORF Names:F12P19.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G65980.
TAIRilocus:2009719. AT1G65980.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002822791 – 162Peroxiredoxin-2BAdd BLAST162

Proteomic databases

PaxDbiQ9XEX2.
PRIDEiQ9XEX2.

Expressioni

Tissue specificityi

Expressed in all tissues but mostly in reproductive tissues such as buds, flowers, siliques and seeds.2 Publications

Inductioni

Slightly induced by salt stress.1 Publication

Gene expression databases

ExpressionAtlasiQ9XEX2. baseline and differential.
GenevisibleiQ9XEX2. AT.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

IntActiQ9XEX2. 2 interactors.
STRINGi3702.AT1G65980.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XEX2.
SMRiQ9XEX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 162ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Belongs to the peroxiredoxin family. Prx5 subfamily.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0541. Eukaryota.
COG0678. LUCA.
HOGENOMiHOG000255884.
InParanoidiQ9XEX2.
OMAiQRYAMVV.
OrthoDBiEOG09360OQV.
PhylomeDBiQ9XEX2.

Family and domain databases

InterProiView protein in InterPro
IPR013740. Redoxin.
IPR036249. Thioredoxin-like_sf.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF08534. Redoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9XEX2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPIAVGDVV PDGTISFFDE NDQLQTASVH SLAAGKKVIL FGVPGAFTPT
60 70 80 90 100
CSMKHVPGFI EKAEELKSKG VDEIICFSVN DPFVMKAWGK TYPENKHVKF
110 120 130 140 150
VADGSGEYTH LLGLELDLKD KGLGVRSRRF ALLLDDLKVT VANVESGGEF
160
TVSSADDILK AL
Length:162
Mass (Da):17,428
Last modified:November 1, 1999 - v1
Checksum:i6EB4690D17598814
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF121355 mRNA. Translation: AAD28242.1.
AC009513 Genomic DNA. Translation: AAF06058.1.
CP002684 Genomic DNA. Translation: AEE34447.1.
AF332464 mRNA. Translation: AAG48827.1.
AY065056 mRNA. Translation: AAL57690.1.
PIRiB96684.
PA0022.
RefSeqiNP_176773.1. NM_105270.3. [Q9XEX2-1]
UniGeneiAt.20543.

Genome annotation databases

EnsemblPlantsiAT1G65980.1; AT1G65980.1; AT1G65980. [Q9XEX2-1]
GeneIDi842910.
GrameneiAT1G65980.1; AT1G65980.1; AT1G65980.
KEGGiath:AT1G65980.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPRX2B_ARATH
AccessioniPrimary (citable) accession number: Q9XEX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: November 1, 1999
Last modified: October 25, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families