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Q9XES5

- DFRA_MALDO

UniProt

Q9XES5 - DFRA_MALDO

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Protein
Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase
Gene
DFR
Organism
Malus domestica (Apple) (Pyrus malus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Bifunctional enzyme involved in the flavonoid metabolism. May use dihydroquercetin, dihydrokaempferol, eriodictyol, garbanzol (5-deoxydihydrokaempferol), dihydrofisetin (5-deoxydihydroquercetin), naringenin to a low extent (10%), but not 5-deoxynaringenin or butin (5-deoxyeriodictyol) as substrate.

Catalytic activityi

(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH.
Cis-3,4-leucopelargonidin + NADP+ = (+)-dihydrokaempferol + NADPH.

Kineticsi

  1. KM=3.9 µM for eriodictyol1 Publication
  2. KM=34.0 µM for dihydrokaempferol
  3. KM=26.0 µM for dihydroquercetin

Vmax=4.6 nmol/sec/g enzyme toward eriodictyol

Vmax=186.0 nmol/sec/g enzyme toward dihydrokaempferol

Vmax=91.0 nmol/sec/g enzyme toward dihydroquercetin

pH dependencei

Optimum pH is 5.75 with dihydroquercetin as substrate.

GO - Molecular functioni

  1. coenzyme binding Source: InterPro
  2. dihydrokaempferol 4-reductase activity Source: UniProtKB-EC
  3. flavanone 4-reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular metabolic process Source: InterPro
  2. flavonoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Flavonoid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.234. 3165.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase
Alternative name(s):
Dihydroflavonol 4-reductase (EC:1.1.1.219)
Short name:
DFR
Dihydrokaempferol 4-reductase
Flavanone 4-reductase (EC:1.1.1.234)
Short name:
FNR
Gene namesi
Name:DFR
OrganismiMalus domestica (Apple) (Pyrus malus)
Taxonomic identifieri3750 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeMaleaeMalus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase
PRO_0000367056Add
BLAST

Expressioni

Developmental stagei

Expressed during fruit ripening.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9XES5.
SMRiQ9XES5. Positions 5-326.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XES5-1 [UniParc]FASTAAdd to Basket

« Hide

MGSESESVCV TGASGFIGSW LVMRLLEHGY TVRATVRDPT NQKKVKHLLD    50
LPKAETHLTL WKADLADEGS FDEAIQGCSG VFHVATPMDF ESKDPENEVI 100
KPTINGLLDI LKACQKAKTV RKLVFTSSAG TVNVEEHQKP VYDESNWSDV 150
EFCRSVKMTG WMYFVSKTLA EQAAWKYAKE NNIDFITIIP TLVIGPFLMP 200
SMPPSLITGL SPILRNESHY GIIKQGQYVH LDDLCLSHIY LYEHPKAEGR 250
YICSSHDATI HELVKMLREK YPEYNIPTKF KGIDDNLEPV HFSSKKLREI 300
GFEFKYSLED MFVGAVDACR AKGLIPIPIP AEKTEAAEES NLVDVKVG 348
Length:348
Mass (Da):39,030
Last modified:November 1, 1999 - v1
Checksum:i02056428FB2F9372
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti347 – 3471V → A in strain: cv. M9.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF117268 mRNA. Translation: AAD26204.1.
AY227729 mRNA. Translation: AAO39817.1.
AY227728 mRNA. Translation: AAO39816.1.
AB074488 mRNA. Translation: BAB92999.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF117268 mRNA. Translation: AAD26204.1 .
AY227729 mRNA. Translation: AAO39817.1 .
AY227728 mRNA. Translation: AAO39816.1 .
AB074488 mRNA. Translation: BAB92999.1 .

3D structure databases

ProteinModelPortali Q9XES5.
SMRi Q9XES5. Positions 5-326.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 1.1.1.234. 3165.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01370. Epimerase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of anthocyanin biosynthesis genes from 'Fuji apple'."
    Lee J.-R., Hong S.-T., Yoo Y.G., Kim S.-R.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Fuji.
    Tissue: Peelings.
  2. "Molecular cloning, substrate specificity of the functionally expressed dihydroflavonol 4-reductases from Malus domestica and Pyrus communis cultivars and the consequences for flavonoid metabolism."
    Fischer T.C., Halbwirth H., Meisel B., Stich K., Forkmann G.
    Arch. Biochem. Biophys. 412:223-230(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. M9 and cv. Weirouge.
  3. "Anthocyanin biosynthetic genes are coordinately expressed during red coloration in apple skin."
    Honda C., Kotoda N., Wada M., Kondo S., Kobayashi S., Soejima J., Zhang Z., Tsuda T., Moriguchi T.
    Plant Physiol. Biochem. 40:955-962(2002)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-317, DEVELOPMENTAL STAGE.
    Strain: cv. Jonathan.
    Tissue: Peelings.

Entry informationi

Entry nameiDFRA_MALDO
AccessioniPrimary (citable) accession number: Q9XES5
Secondary accession number(s): Q84KP1, Q8L5N3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 1, 1999
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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