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Protein

Calcium-transporting ATPase 4, endoplasmic reticulum-type

Gene

ECA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi336Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi337Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi339Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi341Calcium 2By similarity1
Active sitei3834-aspartylphosphate intermediateBy similarity1
Metal bindingi731MagnesiumBy similarity1
Metal bindingi735MagnesiumBy similarity1
Metal bindingi797Calcium 1By similarity1
Metal bindingi800Calcium 1By similarity1
Metal bindingi825Calcium 2By similarity1
Metal bindingi828Calcium 1By similarity1
Metal bindingi829Calcium 1By similarity1
Metal bindingi829Calcium 2By similarity1
Metal bindingi961Calcium 1By similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • cation-transporting ATPase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G07670-MONOMER.
BRENDAi3.6.3.8. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 4, endoplasmic reticulum-type (EC:3.6.3.8)
Gene namesi
Name:ECA4
Ordered Locus Names:At1g07670
ORF Names:F24B9.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G07670.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 70CytoplasmicSequence analysisAdd BLAST70
Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
Topological domaini92 – 115LumenalSequence analysisAdd BLAST24
Transmembranei116 – 135HelicalSequence analysisAdd BLAST20
Topological domaini136 – 278CytoplasmicSequence analysisAdd BLAST143
Transmembranei279 – 298HelicalSequence analysisAdd BLAST20
Topological domaini299 – 327LumenalSequence analysisAdd BLAST29
Transmembranei328 – 345HelicalSequence analysisAdd BLAST18
Topological domaini346 – 786CytoplasmicSequence analysisAdd BLAST441
Transmembranei787 – 806HelicalSequence analysisAdd BLAST20
Topological domaini807 – 816LumenalSequence analysis10
Transmembranei817 – 837HelicalSequence analysisAdd BLAST21
Topological domaini838 – 857CytoplasmicSequence analysisAdd BLAST20
Transmembranei858 – 880HelicalSequence analysisAdd BLAST23
Topological domaini881 – 950LumenalSequence analysisAdd BLAST70
Transmembranei951 – 970HelicalSequence analysisAdd BLAST20
Topological domaini971 – 983CytoplasmicSequence analysisAdd BLAST13
Transmembranei984 – 1002HelicalSequence analysisAdd BLAST19
Topological domaini1003 – 1017LumenalSequence analysisAdd BLAST15
Transmembranei1018 – 1038HelicalSequence analysisAdd BLAST21
Topological domaini1039 – 1061CytoplasmicSequence analysisAdd BLAST23

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464081 – 1061Calcium-transporting ATPase 4, endoplasmic reticulum-typeAdd BLAST1061

Proteomic databases

PaxDbiQ9XES1.

PTM databases

SwissPalmiQ9XES1.

Expressioni

Gene expression databases

GenevisibleiQ9XES1. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G07670.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XES1.
SMRiQ9XES1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiQ9XES1.
KOiK01537.
OMAiITENSMQ.
OrthoDBiEOG093600UE.
PhylomeDBiQ9XES1.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XES1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKGGEDCGN KQTNSSELVK SDTFPAWGKD VSECEEKFGV SREKGLSTDE
60 70 80 90 100
VLKRHQIYGL NELEKPEGTS IFKLILEQFN DTLVRILLAA AVISFVLAFF
110 120 130 140 150
DGDEGGEMGI TAFVEPLVIF LILIVNAIVG IWQETNAEKA LEALKEIQSQ
160 170 180 190 200
QATVMRDGTK VSSLPAKELV PGDIVELRVG DKVPADMRVV ALISSTLRVE
210 220 230 240 250
QGSLTGESEA VSKTTKHVDE NADIQGKKCM VFAGTTVVNG NCICLVTDTG
260 270 280 290 300
MNTEIGRVHS QIQEAAQHEE DTPLKKKLNE FGEVLTMIIG LICALVWLIN
310 320 330 340 350
VKYFLSWEYV DGWPRNFKFS FEKCTYYFEI AVALAVAAIP EGLPAVITTC
360 370 380 390 400
LALGTRKMAQ KNALVRKLPS VETLGCTTVI CSDKTGTLTT NQMAVSKLVA
410 420 430 440 450
MGSRIGTLRS FNVEGTSFDP RDGKIEDWPT GRMDANLQMI AKIAAICNDA
460 470 480 490 500
NVEKSDQQFV SRGMPTEAAL KVLVEKMGFP EGLNEASSDG NVLRCCRLWS
510 520 530 540 550
ELEQRIATLE FDRDRKSMGV MVDSSSGKKL LLVKGAVENV LERSTHIQLL
560 570 580 590 600
DGSTRELDQY SRDLILQSLH DMSLSALRCL GFAYSDVPSD FATYDGSEDH
610 620 630 640 650
PAHQQLLNPS NYSSIESNLV FVGFVGLRDP PRKEVRQAIA DCRTAGIRVM
660 670 680 690 700
VITGDNKSTA EAICREIGVF EADEDISSRS LTGKEFMDVK DQKNHLRQTG
710 720 730 740 750
GLLFSRAEPK HKQEIVRLLK EDGEVVAMTG DGVNDAPALK LADIGVAMGI
760 770 780 790 800
SGTEVAKEAS DLVLADDNFS TIVAAVGEGR SIYNNMKAFI RYMISSNIGE
810 820 830 840 850
VASIFLTAAL GIPEGMIPVQ LLWVNLVTDG PPATALGFNP PDKDIMKKPP
860 870 880 890 900
RRSDDSLITA WILFRYMVIG LYVGVATVGV FIIWYTHNSF MGIDLSQDGH
910 920 930 940 950
SLVSYSQLAH WGQCSSWEGF KVSPFTAGSQ TFSFDSNPCD YFQQGKIKAS
960 970 980 990 1000
TLSLSVLVAI EMFNSLNALS EDGSLVTMPP WVNPWLLLAM AVSFGLHFVI
1010 1020 1030 1040 1050
LYVPFLAQVF GIVPLSLNEW LLVLAVSLPV ILIDEVLKFV GRCTSGYRYS
1060
PRTPSAKQKE E
Length:1,061
Mass (Da):116,180
Last modified:January 11, 2001 - v2
Checksum:i55B6126E5D539822
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti314P → H in AAD29957 (Ref. 3) Curated1
Sequence conflicti430T → M in AAD29957 (Ref. 3) Curated1
Sequence conflicti439M → S in AAD29957 (Ref. 3) Curated1
Sequence conflicti659T → I in AAD29957 (Ref. 3) Curated1
Sequence conflicti859T → P in AAD29957 (Ref. 3) Curated1
Sequence conflicti1046G → V in AAD29957 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007583 Genomic DNA. Translation: AAF75088.1.
CP002684 Genomic DNA. Translation: AEE28160.1.
AF117125 mRNA. Translation: AAD29957.1.
PIRiF86211.
T52332.
RefSeqiNP_172246.3. NM_100640.4.
UniGeneiAt.26214.

Genome annotation databases

EnsemblPlantsiAT1G07670.1; AT1G07670.1; AT1G07670.
GeneIDi837280.
GrameneiAT1G07670.1; AT1G07670.1; AT1G07670.
KEGGiath:AT1G07670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007583 Genomic DNA. Translation: AAF75088.1.
CP002684 Genomic DNA. Translation: AEE28160.1.
AF117125 mRNA. Translation: AAD29957.1.
PIRiF86211.
T52332.
RefSeqiNP_172246.3. NM_100640.4.
UniGeneiAt.26214.

3D structure databases

ProteinModelPortaliQ9XES1.
SMRiQ9XES1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G07670.1.

PTM databases

SwissPalmiQ9XES1.

Proteomic databases

PaxDbiQ9XES1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G07670.1; AT1G07670.1; AT1G07670.
GeneIDi837280.
GrameneiAT1G07670.1; AT1G07670.1; AT1G07670.
KEGGiath:AT1G07670.

Organism-specific databases

TAIRiAT1G07670.

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiQ9XES1.
KOiK01537.
OMAiITENSMQ.
OrthoDBiEOG093600UE.
PhylomeDBiQ9XES1.

Enzyme and pathway databases

BioCyciARA:AT1G07670-MONOMER.
BRENDAi3.6.3.8. 399.

Miscellaneous databases

PROiQ9XES1.

Gene expression databases

GenevisibleiQ9XES1. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECA4_ARATH
AccessioniPrimary (citable) accession number: Q9XES1
Secondary accession number(s): Q9LQP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: November 30, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.